FastQCFastQC Report
Thu 26 May 2016
SRR1546726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546726_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences746778
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30110.4031988087490526No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27840.37280155548235216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGA302.5265217E-644.02
CGTTTTT34750.041.087771
GAGCGAT7850.039.5159237
TGATTCG900.039.1111115
AGCGATA1300.038.923088
TTTAGCG800.038.51
GGTACGA404.1219573E-738.52
TATACGG404.1219573E-738.52
GTTTTTA19300.038.1865272
GCGTTAG357.2867806E-637.7142871
GCGATAA700.037.7142879
ACATCGC357.2867806E-637.71428740
TCGAAGA357.2867806E-637.7142872
TTCGGCA1000.037.418
GCGATAT900.036.6666689
ATTACGA301.3006588E-436.66666411
AGCGATG4400.036.5000048
GCGATGT2550.036.2352949
AGACCGA2150.035.8139538
GCGAGTT800.035.755
Produced by FastQC (version 0.11.5)