##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546726_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 746778 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.470321835940535 31.0 31.0 33.0 30.0 34.0 2 31.858679554030783 31.0 31.0 34.0 30.0 34.0 3 31.975411702005147 31.0 31.0 34.0 30.0 34.0 4 35.67656251255393 37.0 35.0 37.0 33.0 37.0 5 35.62898076804619 37.0 35.0 37.0 33.0 37.0 6 35.658411201186965 37.0 35.0 37.0 33.0 37.0 7 35.99247433641591 37.0 35.0 37.0 35.0 37.0 8 18.52095267937727 30.0 0.0 37.0 0.0 37.0 9 27.925017876798726 30.0 17.0 39.0 17.0 39.0 10 33.54958367814799 32.0 32.0 39.0 27.0 39.0 11 35.71373152396027 37.0 35.0 39.0 32.0 39.0 12 36.487231814541936 37.0 35.0 39.0 32.0 39.0 13 36.73924914767173 39.0 35.0 39.0 33.0 39.0 14 37.85598933016238 40.0 37.0 41.0 33.0 41.0 15 38.05573276127578 40.0 37.0 41.0 33.0 41.0 16 38.11957234948003 40.0 37.0 41.0 33.0 41.0 17 38.08392052256494 40.0 37.0 41.0 33.0 41.0 18 38.05056121096229 40.0 37.0 41.0 33.0 41.0 19 38.046625637070186 40.0 37.0 41.0 33.0 41.0 20 37.96108214221629 40.0 36.0 41.0 33.0 41.0 21 37.81682641963207 40.0 36.0 41.0 33.0 41.0 22 37.815741759934006 40.0 36.0 41.0 33.0 41.0 23 37.66858022062782 39.0 36.0 41.0 33.0 41.0 24 37.5219623502567 39.0 35.0 41.0 33.0 41.0 25 37.368389266957514 39.0 35.0 41.0 32.0 41.0 26 37.33868030391897 39.0 35.0 41.0 32.0 41.0 27 37.2062406766134 39.0 35.0 41.0 32.0 41.0 28 37.17522744376508 39.0 35.0 41.0 32.0 41.0 29 37.06734665456133 39.0 35.0 41.0 32.0 41.0 30 36.95480718500009 39.0 35.0 41.0 31.0 41.0 31 36.96781372777452 39.0 35.0 41.0 31.0 41.0 32 36.776601613866504 39.0 35.0 41.0 31.0 41.0 33 36.65130601062163 39.0 35.0 41.0 30.0 41.0 34 36.47670659821259 39.0 35.0 41.0 30.0 41.0 35 36.302160749245424 39.0 35.0 41.0 30.0 41.0 36 36.16746877920882 39.0 35.0 41.0 30.0 41.0 37 36.104573246667684 39.0 35.0 41.0 29.0 41.0 38 35.998370332280814 39.0 35.0 41.0 29.0 41.0 39 35.88629954283602 39.0 35.0 40.0 29.0 41.0 40 35.810567531448434 39.0 35.0 40.0 28.0 41.0 41 35.75861367099727 38.0 35.0 40.0 28.0 41.0 42 35.58639113632164 38.0 35.0 40.0 27.0 41.0 43 35.578305734769906 38.0 35.0 40.0 27.0 41.0 44 35.323295544325084 38.0 34.0 40.0 27.0 41.0 45 35.214431865962844 38.0 34.0 40.0 26.0 41.0 46 35.20841133509557 38.0 34.0 40.0 26.0 41.0 47 35.06819295694303 38.0 34.0 40.0 26.0 41.0 48 35.02427763003195 37.0 34.0 40.0 26.0 41.0 49 34.99238729582285 37.0 34.0 40.0 26.0 41.0 50 34.36801030560622 37.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 2.0 12 4.0 13 4.0 14 6.0 15 16.0 16 34.0 17 107.0 18 222.0 19 469.0 20 886.0 21 1437.0 22 2228.0 23 3427.0 24 4988.0 25 7323.0 26 9887.0 27 11733.0 28 12934.0 29 14567.0 30 17585.0 31 22100.0 32 28946.0 33 37770.0 34 55723.0 35 77884.0 36 84384.0 37 121470.0 38 124927.0 39 105712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.22058228817667 24.778448213525305 27.40506549469856 14.595904003599463 2 32.916877572719066 25.707372204323104 28.069921717029693 13.305828505928133 3 29.554566417328843 24.803087396789945 31.611134768297944 14.03121141758327 4 25.424021596779767 27.741042183888652 31.030105332508455 15.804830886823126 5 23.183328914349378 31.235253314907506 30.2918671948022 15.289550575940908 6 21.55821408772085 39.74273478865205 27.72711568899994 10.97193543462716 7 84.51762103329236 4.20191810685371 8.84720760386621 2.433253255987723 8 43.409955836942174 50.38940622246504 4.3813556371505324 1.8192823034422545 9 78.46307738042631 5.472844674053065 11.457621943870869 4.606456001649754 10 42.66180846248818 24.861337639834062 17.64071785724807 14.836136040429688 11 33.05721379044375 24.594323882064014 25.600379229168507 16.748083098323733 12 28.799857521244597 21.89499422853914 29.86898382116238 19.436164429053882 13 24.366947071284905 25.165042355291668 31.62305263411616 18.844957939307264 14 20.667855775076394 27.562675922429424 32.111283406849154 19.658184895645025 15 20.32759401053593 22.507224369223515 38.15511437133927 19.01006724890128 16 22.741028793028182 21.26548987784857 35.58326570948796 20.410215619635284 17 22.719201690462228 22.349212215678556 30.24218710245883 24.689398991400388 18 24.89508260821824 22.391661243368176 31.845207009312006 20.86804913910158 19 27.615837638494973 24.72314395978457 27.316150181178344 20.344868220542114 20 28.890781463835303 23.65830273521716 26.747440337021178 20.70347546392636 21 26.43248729876884 25.697730784784767 28.258732849655456 19.611049066790933 22 24.802283945161747 23.23782971646192 28.38246440039744 23.57742193797889 23 23.338930713009756 26.183685111237875 27.30302713791783 23.174357037834536 24 23.275190217172977 23.61571979892284 31.510971131983002 21.598118851921186 25 23.337323809753364 24.033648554188794 29.48238967939602 23.14663795666182 26 21.398862848128893 26.280902758249443 28.758479762392568 23.561754631229093 27 22.25574400959857 24.789964353529427 29.696107812495814 23.258183824376186 28 21.73001882754982 25.34099826186631 31.25065280444791 21.67833010613596 29 22.967602152179094 24.30079622056354 29.92870705885819 22.802894568399175 30 24.065117076293088 24.645878694873176 28.749240068668332 22.539764160165404 31 26.512698552983615 24.176662944007457 26.668969894667494 22.641668608341433 32 27.67716777944717 24.49549933179606 25.74727696852344 22.08005592023332 33 26.587419554405727 25.08161729456411 25.58752400311739 22.743439147912767 34 23.19966576412267 26.812921644719047 27.581557035692 22.405855555466285 35 23.231402103436363 26.821625704024488 27.21451355021171 22.732458642327437 36 27.083417026211272 27.062929009692304 24.513175267616347 21.340478696480076 37 24.3674827057037 28.964699013629218 25.30176304068947 21.366055239977612 38 24.618159613700456 29.33549194004108 24.5806652043847 21.465683241873755 39 23.584251276818545 27.8405362771801 25.33483846605015 23.240373979951205 40 24.888387177983283 25.237353001829195 26.710347653519516 23.163912166668005 41 21.651682293800835 25.1142909941107 27.678105139680063 25.5559215724084 42 23.930137202756374 24.927756307764824 26.38910091084633 24.75300557863247 43 22.883507548428046 24.592315252993526 28.19351935916698 24.330657839411447 44 23.5452838728511 25.741251081311983 27.665785548047744 23.04767949778917 45 22.166560878868953 26.182479933795587 27.734078936444302 23.91688025089116 46 23.384593547212155 26.093296803065975 27.594680078952514 22.92742957076936 47 22.84052288631963 25.492582802385716 28.159774390782804 23.50711992051185 48 23.316434067420307 24.383819555476997 28.9977744389899 23.3019719381128 49 23.40976836489559 23.72204323105394 28.67291216398983 24.195276240060632 50 22.201109298881327 25.44651824236922 28.66889490584886 23.683477552900595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 244.0 1 550.0 2 856.0 3 5164.0 4 9472.0 5 6471.0 6 3470.0 7 3550.5 8 3631.0 9 3762.0 10 3893.0 11 3886.5 12 3880.0 13 3786.5 14 3693.0 15 3630.0 16 3567.0 17 3334.5 18 3102.0 19 3063.5 20 3025.0 21 3206.0 22 3387.0 23 3715.5 24 4044.0 25 4592.0 26 5140.0 27 6363.0 28 7586.0 29 8816.5 30 10047.0 31 11298.5 32 12550.0 33 14160.5 34 15771.0 35 18113.0 36 20455.0 37 22740.0 38 25025.0 39 28834.5 40 32644.0 41 36564.0 42 40484.0 43 44251.0 44 48018.0 45 52243.0 46 56468.0 47 59261.0 48 62054.0 49 60668.5 50 59283.0 51 53969.0 52 48655.0 53 45633.0 54 42611.0 55 41242.0 56 39873.0 57 38432.5 58 36992.0 59 35166.0 60 33340.0 61 31645.0 62 29950.0 63 26858.5 64 23767.0 65 20444.5 66 17122.0 67 14601.5 68 12081.0 69 10113.5 70 8146.0 71 6751.5 72 5357.0 73 4354.0 74 3351.0 75 2622.5 76 1894.0 77 1414.5 78 935.0 79 729.0 80 523.0 81 379.0 82 235.0 83 160.0 84 85.0 85 67.5 86 50.0 87 29.5 88 9.0 89 7.0 90 5.0 91 4.0 92 3.0 93 4.0 94 5.0 95 2.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 746778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.036010336260986 #Duplication Level Percentage of deduplicated Percentage of total 1 66.14394977303347 26.481398567942954 2 8.955329344259042 7.170713163827526 3 5.52786177432582 6.639405934029916 4 4.138834273139252 6.6280964695789715 5 3.3111076686230207 6.628177042273213 6 2.6035391188542008 6.254119144398396 7 2.0960255140053277 5.8741549400648845 8 1.6949869721964779 5.4288412750948725 9 1.3150440404903934 4.738420511794062 >10 4.18106183657543 21.95942710948394 >50 0.019154205129373273 0.5404327676488627 >100 0.012097365570548982 0.8074861607298303 >500 3.3603793250827467E-4 0.06968974196145515 >1k 6.720758650165493E-4 0.7796371711711053 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3011 0.4031988087490526 No Hit CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2784 0.37280155548235216 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0457967428070993 0.0 2 0.0 0.0 0.0 0.13457814772261636 0.0 3 0.0 0.0 0.0 0.21907447728776155 0.0 4 0.0 0.0 0.0 0.3482962808224131 0.0 5 0.0 0.0 0.0 0.5687098441571659 0.0 6 0.0 0.0 0.0 1.016901944085123 0.0 7 0.0 0.0 0.0 1.2732030134792403 0.0 8 0.0 0.0 0.0 1.9739467418697392 0.0 9 0.0 0.0 0.0 2.6933037663134156 0.0 10 0.0 0.0 0.0 3.639635875722102 0.0 11 0.0 0.0 0.0 4.193883590571763 0.0 12 0.0 0.0 0.0 4.677695379349686 0.0 13 0.0 0.0 0.0 4.928640104555838 0.0 14 0.0 0.0 0.0 5.054380284368313 0.0 15 0.0 0.0 0.0 5.171014679061247 0.0 16 0.0 0.0 0.0 5.3769661130884945 0.0 17 0.0 0.0 0.0 5.59550495595746 0.0 18 0.0 0.0 0.0 5.924250580493801 0.0 19 0.0 0.0 0.0 6.0747638521756135 0.0 20 0.0 0.0 0.0 6.248978946889169 0.0 21 0.0 0.0 0.0 6.430291197651779 0.0 22 0.0 0.0 0.0 6.6187006044634416 0.0 23 0.0 0.0 0.0 6.831347468725645 0.0 24 0.0 0.0 0.0 6.996456778319661 0.0 25 0.0 0.0 0.0 7.140408528371216 0.0 26 0.0 0.0 0.0 7.28596718167916 0.0 27 0.0 0.0 0.0 7.441301163130141 0.0 28 0.0 0.0 0.0 7.59060925736966 0.0 29 0.0 0.0 0.0 7.7539777551025875 0.0 30 0.0 0.0 0.0 7.949484317963304 0.0 31 0.0 0.0 0.0 8.11298672430093 0.0 32 0.0 0.0 0.0 8.277962125290246 0.0 33 0.0 0.0 0.0 8.447356510234634 0.0 34 0.0 0.0 0.0 8.6200986102965 0.0 35 0.0 0.0 0.0 8.808641925712863 0.0 36 0.0 0.0 0.0 8.980178848332436 0.0 37 0.0 0.0 0.0 9.16550835723602 0.0 38 0.0 0.0 0.0 9.38752882382716 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATGA 30 2.5265217E-6 44.0 2 CGTTTTT 3475 0.0 41.08777 1 GAGCGAT 785 0.0 39.515923 7 TGATTCG 90 0.0 39.11111 15 AGCGATA 130 0.0 38.92308 8 TTTAGCG 80 0.0 38.5 1 GGTACGA 40 4.1219573E-7 38.5 2 TATACGG 40 4.1219573E-7 38.5 2 GTTTTTA 1930 0.0 38.186527 2 GCGTTAG 35 7.2867806E-6 37.714287 1 GCGATAA 70 0.0 37.714287 9 ACATCGC 35 7.2867806E-6 37.714287 40 TCGAAGA 35 7.2867806E-6 37.714287 2 TTCGGCA 100 0.0 37.4 18 GCGATAT 90 0.0 36.666668 9 ATTACGA 30 1.3006588E-4 36.666664 11 AGCGATG 440 0.0 36.500004 8 GCGATGT 255 0.0 36.235294 9 AGACCGA 215 0.0 35.813953 8 GCGAGTT 80 0.0 35.75 5 >>END_MODULE