FastQCFastQC Report
Thu 26 May 2016
SRR1546725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546725_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1483424
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.24780507798175033No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32730.22063819919321787No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21500.14493496127877126No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA18350.12370030416118385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAT15250.040.681977
CTAAACG452.3517714E-839.111111
CTATTAG452.3517714E-839.111111
TATAGCG850.038.823531
AGACCGA4850.038.55678
CTATCTC8050.038.5341646
CGTTTTT41950.038.2312281
TCGTAGA1500.038.133332
TCTAGCG651.0913936E-1137.230771
ACGAGTA651.0913936E-1137.230775
TATACTA8550.036.79532244
CGTATGG900.036.6666682
TTAACGA301.301278E-436.6666642
ACGGTAC301.301278E-436.66666413
AGGGGAA63400.036.504738
TAAGTAG1700.036.235291
CGTTAGA1100.036.02
GTTACGA553.426976E-936.02
TACGAGT553.426976E-936.04
AGTTACG553.426976E-936.01
Produced by FastQC (version 0.11.5)