##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546725_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1483424
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.452683791013225	31.0	31.0	33.0	30.0	34.0
2	31.82699821494057	31.0	31.0	34.0	30.0	34.0
3	31.963139331708263	31.0	31.0	34.0	30.0	34.0
4	35.655269161076	37.0	35.0	37.0	33.0	37.0
5	35.59506317816079	37.0	35.0	37.0	33.0	37.0
6	35.6322076493302	37.0	35.0	37.0	33.0	37.0
7	35.973608354725286	37.0	35.0	37.0	35.0	37.0
8	18.66747942597666	32.0	0.0	37.0	0.0	37.0
9	27.986362631317817	31.0	17.0	39.0	17.0	39.0
10	33.566627612874	32.0	32.0	39.0	27.0	39.0
11	35.71005727290377	37.0	35.0	39.0	32.0	39.0
12	36.41216334642017	37.0	35.0	39.0	32.0	39.0
13	36.60814305282913	39.0	35.0	39.0	33.0	39.0
14	37.76642349051923	39.0	36.0	41.0	33.0	41.0
15	37.9726160558276	40.0	36.0	41.0	33.0	41.0
16	38.03882908730073	40.0	36.0	41.0	33.0	41.0
17	37.9940374431046	40.0	36.0	41.0	33.0	41.0
18	37.986683510580924	40.0	36.0	41.0	33.0	41.0
19	37.99532972366633	40.0	36.0	41.0	33.0	41.0
20	37.92385251957633	40.0	36.0	41.0	33.0	41.0
21	37.784898316327634	40.0	36.0	41.0	33.0	41.0
22	37.750199538365294	40.0	36.0	41.0	33.0	41.0
23	37.613170610695256	39.0	36.0	41.0	33.0	41.0
24	37.49419451215566	39.0	35.0	41.0	32.0	41.0
25	37.32755368660612	39.0	35.0	41.0	32.0	41.0
26	37.294060228228744	39.0	35.0	41.0	32.0	41.0
27	37.19223836205967	39.0	35.0	41.0	32.0	41.0
28	37.18675038289794	39.0	35.0	41.0	32.0	41.0
29	37.09903641844813	39.0	35.0	41.0	32.0	41.0
30	37.00203650473499	39.0	35.0	40.0	31.0	41.0
31	37.070370979571585	39.0	35.0	41.0	32.0	41.0
32	36.92198184740169	39.0	35.0	41.0	31.0	41.0
33	36.8493856105874	39.0	35.0	41.0	31.0	41.0
34	36.75115341264534	39.0	35.0	41.0	31.0	41.0
35	36.60917445046056	39.0	35.0	41.0	31.0	41.0
36	36.42240249584744	39.0	35.0	41.0	30.0	41.0
37	36.35682650408784	39.0	35.0	41.0	30.0	41.0
38	36.25302273658779	39.0	35.0	40.0	30.0	41.0
39	36.118990254977675	38.0	35.0	40.0	30.0	41.0
40	36.022541768233495	38.0	35.0	40.0	30.0	41.0
41	35.98037310977846	38.0	35.0	40.0	29.0	41.0
42	35.831704219427486	38.0	35.0	40.0	29.0	41.0
43	35.83224418642276	38.0	35.0	40.0	29.0	41.0
44	35.57942975170956	38.0	35.0	40.0	28.0	41.0
45	35.48288756282762	38.0	34.0	40.0	28.0	41.0
46	35.46692314537179	38.0	34.0	40.0	28.0	41.0
47	35.330498899842524	38.0	34.0	40.0	28.0	41.0
48	35.28041207368898	37.0	34.0	40.0	28.0	41.0
49	35.22087953275665	37.0	34.0	40.0	28.0	41.0
50	34.6025303621891	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	7.0
13	4.0
14	8.0
15	24.0
16	56.0
17	192.0
18	378.0
19	841.0
20	1555.0
21	2538.0
22	4104.0
23	5934.0
24	8372.0
25	11822.0
26	15769.0
27	19563.0
28	23096.0
29	27682.0
30	35058.0
31	45386.0
32	58471.0
33	77948.0
34	116192.0
35	168790.0
36	166441.0
37	238677.0
38	249583.0
39	204927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.35290517074013	24.759677610716828	28.667663459671676	13.219753758871367
2	30.441532562504044	26.40121772332118	29.9515849817719	13.205664732402871
3	29.85869178333369	25.857475677891152	30.154156869512693	14.129675669262461
4	26.899928813339947	28.161334857734538	29.592011454580753	15.346724874344758
5	23.552740147119096	32.811792178096084	28.755163729318117	14.880303945466705
6	22.56933958194016	40.40341803826822	25.932707034536318	11.094535345255302
7	86.1317465539185	4.758113661367215	6.652177664646117	2.4579621200681667
8	44.53972700994456	50.24982742627866	3.20016394503527	2.010281618741506
9	79.26378432599176	6.444212848113554	9.646196906616044	4.645805919278642
10	44.89377278512415	25.899675345686735	14.317484414435791	14.889067454753327
11	36.73326034903035	23.301564488642494	22.840738723385897	17.12443643894126
12	33.05676596846215	20.72596910930388	26.48339247578575	19.733872446448217
13	23.8389698427422	28.101338524926117	27.731990314299892	20.327701318031796
14	19.397960394330955	30.234983389779323	28.915064067994045	21.45199214789568
15	18.624412170761698	22.56623864788489	38.52856634380999	20.280782837543413
16	20.19705761805121	20.370035809047177	37.085014129473436	22.347892443428176
17	20.78556097245292	22.110738399810167	28.759006191082253	28.34469443665466
18	23.28579017192657	23.077016416075242	30.91112183704726	22.72607157495092
19	28.165109907888773	25.8934734775762	24.19679066807602	21.744625946459003
20	30.19763735789633	22.914554436223224	24.649594451754858	22.238213754125592
21	25.740112065060295	27.408212351964107	24.289953512953815	22.561722070021787
22	24.69469281877602	24.930026748926807	24.428012490023082	25.947267942274088
23	24.596204456716354	27.661612593567313	23.874697995987663	23.86748495372867
24	23.339045343745283	23.487687943568396	31.388463446728647	21.784803265957674
25	22.974550769031644	24.372802381517356	28.410825225963716	24.241821623487283
26	21.32835925534439	28.77073581120435	26.15132288543262	23.749582048018638
27	22.774203464417457	26.545883038160362	26.41355404793235	24.26635944948983
28	21.303147313242874	26.774880276980824	30.329427055245162	21.59254535453114
29	22.882264275082512	24.187353042690425	28.635036240481483	24.295346441745583
30	24.250180663114524	26.44921478956792	26.285674223957546	23.014930323360012
31	27.7736506892163	24.860525379122894	23.54734721832733	23.818476713333478
32	30.201614642880255	24.721050758245788	23.661879543542508	21.41545505533145
33	27.9109681385767	25.740246888280087	23.416366460297258	22.932418512845956
34	24.425450848846992	24.993124015790496	26.768071704381214	23.813353430981294
35	26.338187868067394	23.919728951398923	26.376882132148328	23.365201048385355
36	28.017141424164638	27.345249908320213	23.873484587009514	20.764124080505642
37	26.370343205988306	28.28388916452747	25.02278512414522	20.322982505339
38	25.645398753154865	28.30350594300753	24.228608947947453	21.822486355890156
39	25.531540544038656	27.229571585736785	25.0768492352827	22.162038634941865
40	26.446113855512653	24.23818139655284	26.7986765752745	22.51702817266001
41	21.799970878184524	24.056641931099943	27.57862890178398	26.56475828893155
42	24.415878400241603	25.284139935716286	24.651077507172594	25.648904156869513
43	23.660733546174257	24.463066527169573	27.032797096447137	24.84340283020903
44	23.537033242013074	26.18064693573786	26.886783549410016	23.395536272839053
45	22.040293267467696	27.541754751170267	25.646814396962704	24.771137584399337
46	23.929503634834006	27.665589878551245	25.151204240999203	23.25370224561555
47	24.73149955777984	24.359994175636903	26.843977177125357	24.064529089457903
48	24.077067648898765	22.80130293159609	28.35460394330953	24.767025476195613
49	23.41164764760446	23.60963554587225	28.15135793946977	24.82735886705352
50	21.76006320512544	25.413030933839547	28.290495502297386	24.536410358737623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	527.0
1	1098.0
2	1669.0
3	6476.5
4	11284.0
5	7932.0
6	4580.0
7	4636.0
8	4692.0
9	4885.5
10	5079.0
11	5169.5
12	5260.0
13	5263.0
14	5266.0
15	5235.5
16	5205.0
17	5228.5
18	5252.0
19	5137.0
20	5022.0
21	5123.5
22	5225.0
23	5892.0
24	6559.0
25	8676.0
26	10793.0
27	12390.5
28	13988.0
29	16576.0
30	19164.0
31	21037.5
32	22911.0
33	25069.0
34	27227.0
35	30372.0
36	33517.0
37	36168.0
38	38819.0
39	49063.0
40	59307.0
41	71194.0
42	83081.0
43	89963.5
44	96846.0
45	101326.5
46	105807.0
47	111200.0
48	116593.0
49	121809.5
50	127026.0
51	121227.0
52	115428.0
53	104228.5
54	93029.0
55	88750.5
56	84472.0
57	84857.5
58	85243.0
59	82369.0
60	79495.0
61	71553.0
62	63611.0
63	54391.0
64	45171.0
65	38389.5
66	31608.0
67	27184.5
68	22761.0
69	19536.5
70	16312.0
71	13728.0
72	11144.0
73	9130.0
74	7116.0
75	5553.0
76	3990.0
77	2925.0
78	1860.0
79	1335.5
80	811.0
81	613.0
82	415.0
83	294.0
84	173.0
85	110.0
86	47.0
87	40.0
88	33.0
89	19.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1483424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.913481510465658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65433853020407	20.280355352179637
2	6.730703174487679	3.7575471722698865
3	2.9256612810553033	2.4499617622386745
4	2.001832266211392	2.23512431799781
5	1.5540581382474143	2.168958655407894
6	1.284147287748438	2.1507012943968387
7	1.0808973007522344	2.1120124773481814
8	0.9764943153563315	2.1805884813419025
9	0.8649858030029126	2.1730288697043383
>10	9.71630604439262	54.365893048481375
>50	0.17186599200252753	3.072259345522826
>100	0.03556469068679654	1.9273900952650056
>500	0.0021774294361864258	0.3877740796407875
>1k	9.67746416082856E-4	0.7384050482048712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3676	0.24780507798175033	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3273	0.22063819919321787	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2150	0.14493496127877126	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	1835	0.12370030416118385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.048468947516017	0.0
2	0.0	0.0	0.0	0.16084410121448756	0.0
3	0.0	0.0	0.0	0.24611978773432275	0.0
4	0.0	0.0	0.0	0.4062223612399422	0.0
5	0.0	0.0	0.0	0.6632628297775957	0.0
6	0.0	0.0	0.0	1.187590331557262	0.0
7	0.0	0.0	0.0	1.4674159242401363	0.0
8	0.0	0.0	0.0	2.294960847336972	0.0
9	0.0	0.0	0.0	3.113337791487801	0.0
10	0.0	0.0	0.0	4.223404771663395	0.0
11	0.0	0.0	0.0	4.906284379921048	0.0
12	0.0	0.0	0.0	5.520808615743038	0.0
13	0.0	0.0	0.0	5.827531440774856	0.0
14	0.0	0.0	0.0	5.96309618827793	0.0
15	0.0	0.0	0.0	6.0981890545117245	0.0
16	0.0	0.0	0.0	6.329545699678581	0.0
17	0.0	0.0	0.0	6.574047608775373	0.0
18	0.0	0.0	0.0	6.939620769247363	0.0
19	0.0	0.0	0.0	7.098307698945143	0.0
20	0.0	0.0	0.0	7.302092995664085	0.0
21	0.0	0.0	0.0	7.517001208016049	0.0
22	0.0	0.0	0.0	7.746672562935479	0.0
23	0.0	0.0	0.0	7.987264598658239	0.0
24	0.0	0.0	0.0	8.178578747546217	0.0
25	0.0	0.0	0.0	8.342860840865457	0.0
26	6.741160989710292E-5	0.0	0.0	8.497840132018897	0.0
27	6.741160989710292E-5	0.0	0.0	8.658010117134413	0.0
28	6.741160989710292E-5	0.0	0.0	8.820606920206226	0.0
29	6.741160989710292E-5	0.0	0.0	8.97666479711802	0.0
30	6.741160989710292E-5	0.0	0.0	9.192988653277823	0.0
31	6.741160989710292E-5	0.0	0.0	9.377494229566192	0.0
32	6.741160989710292E-5	0.0	0.0	9.561460512975387	0.0
33	6.741160989710292E-5	0.0	0.0	9.738550812175076	0.0
34	6.741160989710292E-5	0.0	0.0	9.917596048061782	0.0
35	6.741160989710292E-5	0.0	0.0	10.113831244472248	0.0
36	6.741160989710292E-5	0.0	0.0	10.302785987013827	0.0
37	1.3482321979420585E-4	0.0	0.0	10.493830489462217	0.0
38	1.3482321979420585E-4	0.0	0.0	10.696334965593115	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGAT	1525	0.0	40.68197	7
CTAAACG	45	2.3517714E-8	39.11111	1
CTATTAG	45	2.3517714E-8	39.11111	1
TATAGCG	85	0.0	38.82353	1
AGACCGA	485	0.0	38.5567	8
CTATCTC	805	0.0	38.534164	6
CGTTTTT	4195	0.0	38.231228	1
TCGTAGA	150	0.0	38.13333	2
TCTAGCG	65	1.0913936E-11	37.23077	1
ACGAGTA	65	1.0913936E-11	37.23077	5
TATACTA	855	0.0	36.795322	44
CGTATGG	90	0.0	36.666668	2
TTAACGA	30	1.301278E-4	36.666664	2
ACGGTAC	30	1.301278E-4	36.666664	13
AGGGGAA	6340	0.0	36.50473	8
TAAGTAG	170	0.0	36.23529	1
CGTTAGA	110	0.0	36.0	2
GTTACGA	55	3.426976E-9	36.0	2
TACGAGT	55	3.426976E-9	36.0	4
AGTTACG	55	3.426976E-9	36.0	1
>>END_MODULE