FastQCFastQC Report
Thu 26 May 2016
SRR1546724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546724_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1330513
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59680.44854879283404214No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53100.3990941839726482No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.8569294E-444.01
TCCCGTA207.8569294E-444.042
GTATACG408.314601E-944.01
TAAATCG207.8569294E-444.01
CGTTTTT63800.041.6896551
ACGTTAG501.3478711E-939.6000021
GAGCGAT14100.039.4751787
AGCGATA3250.039.261548
GTTTTTA35250.039.06952
CTATCTC5550.038.450456
GCGATAT2100.037.7142839
TGATTCG2700.037.4814815
TTAGTCG301.3012058E-436.6666641
TATACGA601.9826984E-1036.6666642
GACGATT850.036.2352947
ATATGCT5600.036.14285734
TATGCGA553.425157E-936.0000042
TATCTCT6000.035.5666667
AGACCGA3900.035.5384648
TATTTTT41350.035.221287
Produced by FastQC (version 0.11.5)