FastQCFastQC Report
Thu 26 May 2016
SRR1546723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546723_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3069586
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183410.5975072859988285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACA207.858541E-444.030
CGTTTTT303800.043.1237641
GAGCGAT30850.042.2884947
GCGCGAC2650.040.6792459
GTTTTTA170850.039.995322
CGAGATT9100.039.1648376
AGACCGA9050.038.408848
GAGACCG10200.037.74517
TATGCGA2100.037.7142872
ACCCGCT2650.037.3584934
CGAGAAT5250.037.2952356
CGTAAGA2600.037.230772
GTTACGA1600.037.1252
AGCGATC3400.036.882358
TGCGCGA1850.036.8648642
AGCGATG15500.036.6193548
GCGTTCG3150.036.31746344
CGAGATC5300.036.1132056
AGCGATA6250.035.9048
AGCCGAT4800.035.7500048
Produced by FastQC (version 0.11.5)