FastQCFastQC Report
Thu 26 May 2016
SRR1546722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546722_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1186757
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79950.6736846717567286No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.6564949690627483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA207.856583E-444.00000410
GTATGCG302.527786E-644.01
TATGCGA454.802132E-1043.9999962
CGTTTTT105100.042.8696481
GAGCGAT11700.041.1794857
GTTTTTA55950.040.9329762
ATATGCG557.8216544E-1140.01
TACGAAT1050.039.80952512
CGCAGTA501.3478711E-939.637
CTATCTC6650.039.368426
AGACCGA4000.038.58
CGTTGAG1100.038.03
TGTGCGA1400.037.7142832
TCTAGCG357.2904095E-637.7142831
ACGGGTA651.0913936E-1137.230775
AGCGATA2900.037.1724178
GATGTCT10850.036.700467
GCGTTAG1200.036.6666641
ATGTCTC11900.036.605048
TATTTTT64550.036.365617
Produced by FastQC (version 0.11.5)