FastQCFastQC Report
Thu 26 May 2016
SRR1546721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546721_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1997704
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124720.6243167155894968No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117280.5870739609071214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT143550.041.9003831
AGCGATA3900.040.6153838
GAGCGAT21450.040.4102557
GTTTTTA76000.040.1499982
CTTGCGA900.039.111112
ATCGACG404.1265776E-738.5000041
TATCTCT12850.038.0077827
CGTTAGA1100.038.02
CTATCTC12850.037.8365756
ATGTCTC19000.037.6315778
TCGATAG1000.037.41
CGGTCTA1300.037.2307731
TATAGCG900.036.6666681
TCGTTAG850.036.2352941
TATTTTT87850.035.9612967
ATCTCTT13650.035.941398
TTATTTT89650.035.2883456
TACGGTA250.002353158835.214
TCCGTTA250.002353158835.216
TCGTAAG1200.034.8333321
Produced by FastQC (version 0.11.5)