FastQCFastQC Report
Thu 26 May 2016
SRR1546720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546720_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2722486
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166640.6120876287334444No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154330.5668716019109006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT172150.042.2619821
GTTTTTA91450.040.4876982
AGCGATA5950.039.9327748
GAGCGAT26900.039.747217
CTATCTC14700.039.2108846
CGCATGA1750.037.7142872
GTTTACG850.036.235291
TATCTCT16150.035.8266227
TATTTTT107700.035.624887
TTATTTT108050.035.611296
GACCGAT19050.035.4540679
AGACCGA10750.035.28
AGCCGAT2950.035.050858
TATACTA14700.034.5714344
ATGTCTC24000.034.5583348
CTAATCC17100.034.4795311
AGCGATG12100.034.3636368
TTTATTT112600.034.309065
GTTACGA2000.034.1000022
GAGACCG12450.033.927717