##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546720_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2722486
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.448751251613416	31.0	31.0	33.0	30.0	34.0
2	31.831619703462202	31.0	31.0	34.0	30.0	34.0
3	31.938100324482843	31.0	31.0	34.0	30.0	34.0
4	35.65373118539453	37.0	35.0	37.0	33.0	37.0
5	35.599434487450075	37.0	35.0	37.0	33.0	37.0
6	35.61677011378571	37.0	35.0	37.0	33.0	37.0
7	35.9785368960575	37.0	35.0	37.0	35.0	37.0
8	18.69898467797447	32.0	0.0	37.0	0.0	37.0
9	28.013846168538606	31.0	17.0	39.0	17.0	39.0
10	33.56552356926721	32.0	32.0	39.0	27.0	39.0
11	35.72276184340342	37.0	35.0	39.0	32.0	39.0
12	36.48363444293194	37.0	35.0	39.0	32.0	39.0
13	36.74538491657992	39.0	35.0	39.0	33.0	39.0
14	37.858055835732486	40.0	37.0	41.0	33.0	41.0
15	38.04916425649204	40.0	37.0	41.0	33.0	41.0
16	38.09910170336964	40.0	37.0	41.0	33.0	41.0
17	38.02852650114638	40.0	37.0	41.0	33.0	41.0
18	37.9824289270909	40.0	37.0	41.0	33.0	41.0
19	37.96979782448835	40.0	37.0	41.0	33.0	41.0
20	37.88311785625344	40.0	36.0	41.0	33.0	41.0
21	37.73758359087981	40.0	36.0	41.0	33.0	41.0
22	37.72138883358812	40.0	36.0	41.0	33.0	41.0
23	37.546605198337105	39.0	36.0	41.0	32.0	41.0
24	37.36921806025816	39.0	35.0	41.0	32.0	41.0
25	37.21375940959843	39.0	35.0	41.0	32.0	41.0
26	37.1877342987255	39.0	35.0	41.0	32.0	41.0
27	37.06535827916103	39.0	35.0	41.0	32.0	41.0
28	37.0193360039317	39.0	35.0	41.0	32.0	41.0
29	36.91548276097655	39.0	35.0	40.0	31.0	41.0
30	36.77269745372428	39.0	35.0	40.0	31.0	41.0
31	36.76952865873323	39.0	35.0	41.0	31.0	41.0
32	36.52469654573063	39.0	35.0	41.0	30.0	41.0
33	36.36551335801176	39.0	35.0	41.0	30.0	41.0
34	36.14690066358468	39.0	35.0	41.0	30.0	41.0
35	35.93281985655757	39.0	35.0	41.0	28.0	41.0
36	35.781217975041926	39.0	35.0	41.0	27.0	41.0
37	35.713024052281625	39.0	35.0	40.0	27.0	41.0
38	35.59084638084457	38.0	35.0	40.0	27.0	41.0
39	35.452528314195185	38.0	35.0	40.0	26.0	41.0
40	35.35892415975693	38.0	35.0	40.0	26.0	41.0
41	35.30541167153844	38.0	35.0	40.0	26.0	41.0
42	35.129621970507834	38.0	34.0	40.0	25.0	41.0
43	35.11256439886192	38.0	34.0	40.0	25.0	41.0
44	34.842937667999024	38.0	34.0	40.0	24.0	41.0
45	34.735317279868475	38.0	34.0	40.0	23.0	41.0
46	34.723583518886784	37.0	34.0	40.0	24.0	41.0
47	34.585686023729785	37.0	34.0	40.0	23.0	41.0
48	34.541831620070774	37.0	34.0	40.0	23.0	41.0
49	34.49285689623381	37.0	34.0	40.0	24.0	41.0
50	33.88363576525279	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	7.0
13	14.0
14	22.0
15	58.0
16	156.0
17	358.0
18	969.0
19	1953.0
20	3597.0
21	6028.0
22	9557.0
23	14210.0
24	21305.0
25	31010.0
26	42747.0
27	50409.0
28	53735.0
29	58293.0
30	68007.0
31	84939.0
32	107181.0
33	140410.0
34	204509.0
35	281734.0
36	305451.0
37	431816.0
38	435777.0
39	368229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.5400755045205	23.123351231190902	27.13927638195385	17.197296882334747
2	34.08351778484811	24.669805464564373	27.26724765526802	13.979429095319498
3	28.093037025718402	24.96497686305825	33.18683732441599	13.755148786807354
4	25.02359975404832	27.440067644057674	32.08100978297041	15.455322818923586
5	23.28195627084951	30.039162735823066	31.59145721961472	15.087423773712702
6	21.528779211353154	38.07604520280361	29.26556830778928	11.129607278053955
7	82.24641008254954	4.1627394961810635	10.9277329617122	2.663117459557184
8	42.50633428417998	49.87970553383929	5.487080557990014	2.12687962399072
9	75.58679824248867	5.411561345035383	14.094507740352016	4.907132672123934
10	40.789961821658586	25.205051559493786	19.416041074224072	14.588945544623556
11	31.889052872999162	23.705466254004612	27.433015266194204	16.972465606802018
12	27.739316198503865	20.816525778277647	31.68460737722802	19.75955064599047
13	23.82623822491649	23.974705471396362	32.932437485445284	19.266618818241856
14	20.549857740315286	25.520094501863372	33.749668501509284	20.18037925631206
15	19.900671665529227	21.597429702117843	38.56107983659053	19.940818795762404
16	23.32225032562151	20.975204280205663	35.58905353415959	20.113491860013237
17	22.788473476080316	22.712990994260394	31.611953192780422	22.886582336878867
18	24.987199199555114	21.153864519413506	33.15851761955801	20.700418661473375
19	26.489135297665445	25.380112147500483	28.132339339853356	19.99841321498072
20	28.373405776925942	23.435382220514633	27.440030912922968	20.751181089636457
21	25.57214251974115	26.443625421765255	28.488006917207287	19.49622514128631
22	24.651366434942183	22.68121856274008	29.33469630330514	23.332718699012595
23	23.1041775788746	26.17736142628465	28.41825449240143	22.300206502439316
24	22.983258683423898	22.90762927706515	32.838295587194935	21.27081645231601
25	24.000270341151435	23.455033377582108	29.394972095356962	23.149724185909495
26	21.55974355790994	24.539409936359636	30.18237743004004	23.71846907569038
27	22.316698781922113	23.11758444304213	30.34910005046858	24.216616724567178
28	21.70450095978455	24.996786025713263	30.965448490827868	22.333264523674316
29	22.81921743582887	23.79611869445793	29.749096965053262	23.63556690465993
30	24.050849113640986	23.026050455355875	30.32173535511294	22.601365075890197
31	26.15469831617132	22.827298285464096	27.373694483644726	23.644308914719854
32	26.119473157988693	23.035049583358738	27.539535556840328	23.30594170181224
33	25.018383932920134	23.852427523961556	26.858503588264547	24.270684954853763
34	21.549091528845327	24.085633498207155	31.1116751380907	23.25359983485682
35	21.732637008969007	25.706835590706433	29.701383221070742	22.859144179253814
36	24.394579072215613	26.53802443795854	27.580454040902325	21.48694244892352
37	22.903478658843422	27.309598653583528	27.61446707163967	22.17245561593338
38	24.38833477931567	26.595618857176863	27.542216929673835	21.473829433833636
39	23.56695314502995	25.122186119598044	27.495641850867187	23.815218884504823
40	25.479947371630196	23.012239548706585	28.77278340457949	22.73502967508373
41	21.44003678990452	23.30990866436044	29.0747500629939	26.17530448274114
42	23.703299117056982	24.40592899283963	27.348827505449062	24.541944384654318
43	22.678022954020697	23.225243398864126	29.414953832636787	24.681779814478386
44	23.42910119647998	24.378454104079875	29.013115218957967	23.179329480482178
45	22.28569770423062	24.862276610421503	28.430118648911325	24.42190703643655
46	23.358136644228843	25.03744739183232	27.98086014032763	23.623555823611213
47	22.476736335834232	24.546095002876047	29.08415323347852	23.8930154278112
48	22.617820624238288	23.585392174652135	29.824726371412012	23.972060829697565
49	23.7364306005614	22.773303517446923	29.162096701323716	24.328169180667963
50	21.88889125600646	24.217792120877757	30.107923419991877	23.78539320312391
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1078.0
1	2155.5
2	3233.0
3	27175.5
4	51118.0
5	34429.0
6	17740.0
7	17516.5
8	17293.0
9	17767.0
10	18241.0
11	17972.0
12	17703.0
13	17245.5
14	16788.0
15	16128.5
16	15469.0
17	14888.0
18	14307.0
19	14167.0
20	14027.0
21	14063.0
22	14099.0
23	14896.5
24	15694.0
25	17605.5
26	19517.0
27	22149.5
28	24782.0
29	28250.0
30	31718.0
31	35921.0
32	40124.0
33	46195.5
34	52267.0
35	60092.5
36	67918.0
37	74519.5
38	81121.0
39	93216.0
40	105311.0
41	118118.5
42	130926.0
43	144704.0
44	158482.0
45	177691.0
46	196900.0
47	213912.5
48	230925.0
49	226588.0
50	222251.0
51	203615.5
52	184980.0
53	172446.5
54	159913.0
55	152979.0
56	146045.0
57	142462.0
58	138879.0
59	133077.0
60	127275.0
61	120586.0
62	113897.0
63	100765.0
64	87633.0
65	74630.0
66	61627.0
67	52794.0
68	43961.0
69	37114.5
70	30268.0
71	25269.0
72	20270.0
73	16824.5
74	13379.0
75	10761.5
76	8144.0
77	6077.5
78	4011.0
79	2936.5
80	1862.0
81	1312.0
82	762.0
83	534.0
84	306.0
85	232.0
86	158.0
87	100.5
88	43.0
89	34.5
90	26.0
91	15.0
92	4.0
93	6.0
94	8.0
95	4.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2722486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.00180992088533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.67224672279876	28.370118994442233
2	6.59170228791565	4.878098302250397
3	2.924361807596042	3.246200392336961
4	1.9393051553254166	2.8703120294377626
5	1.477627964177396	2.7337454532138383
6	1.181965596481443	2.624091980041933
7	0.9785071645056789	2.534457527508452
8	0.8693243185674455	2.573325855618863
9	0.7729584720062468	2.57407762121218
>10	6.505655651573799	42.551976347950436
>50	0.06723943679398359	1.6465342490201824
>100	0.016252959847408308	1.0602667864895636
>500	0.002108341781829969	0.5458118949160515
>1k	4.960804192541099E-4	0.31806139596966126
>5k	0.0	0.0
>10k+	2.4804020962705496E-4	1.4729211695914322
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16664	0.6120876287334444	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15433	0.5668716019109006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.673113470555955E-5	0.0	0.0	0.04532622022666049	0.0
2	3.673113470555955E-5	0.0	0.0	0.140019085497593	0.0
3	3.673113470555955E-5	0.0	0.0	0.23798102175732033	0.0
4	3.673113470555955E-5	0.0	0.0	0.3927292922718427	0.0
5	3.673113470555955E-5	0.0	0.0	0.6466883576260815	0.0
6	3.673113470555955E-5	0.0	0.0	1.2815860210116783	0.0
7	3.673113470555955E-5	0.0	0.0	1.6709727800253151	0.0
8	3.673113470555955E-5	0.0	0.0	2.63791990114917	0.0
9	3.673113470555955E-5	0.0	0.0	3.716235822700282	0.0
10	7.34622694111191E-5	0.0	0.0	4.916315455800324	0.0
11	7.34622694111191E-5	0.0	0.0	5.602379589830765	0.0
12	7.34622694111191E-5	0.0	0.0	6.207414840700742	0.0
13	7.34622694111191E-5	0.0	0.0	6.545818784743062	0.0
14	7.34622694111191E-5	0.0	0.0	6.735645288901393	0.0
15	7.34622694111191E-5	0.0	0.0	6.87522360078252	0.0
16	1.1019340411667866E-4	0.0	0.0	7.075995983083109	0.0
17	1.1019340411667866E-4	0.0	0.0	7.28352689416952	0.0
18	1.1019340411667866E-4	0.0	0.0	7.6101768751060614	0.0
19	1.1019340411667866E-4	0.0	0.0	7.768157485474672	0.0
20	1.1019340411667866E-4	0.0	0.0	7.956661668783604	0.0
21	1.1019340411667866E-4	0.0	0.0	8.14174985656492	0.0
22	1.1019340411667866E-4	0.0	0.0	8.34002452170553	0.0
23	1.1019340411667866E-4	0.0	0.0	8.552183555764842	0.0
24	1.1019340411667866E-4	0.0	0.0	8.733341512132661	0.0
25	1.1019340411667866E-4	0.0	0.0	8.892644443350672	0.0
26	1.1019340411667866E-4	0.0	0.0	9.042360548410533	0.0
27	1.1019340411667866E-4	0.0	0.0	9.202545026861479	0.0
28	1.1019340411667866E-4	0.0	0.0	9.358909467303047	0.0
29	1.1019340411667866E-4	0.0	0.0	9.529745974818603	0.0
30	1.1019340411667866E-4	0.0	0.0	9.7490675801455	0.0
31	1.469245388222382E-4	0.0	0.0	9.923136427515146	0.0
32	1.469245388222382E-4	0.0	0.0	10.134854687957992	0.0
33	1.469245388222382E-4	3.673113470555955E-5	0.0	10.318804210563433	0.0
34	1.469245388222382E-4	3.673113470555955E-5	0.0	10.50668396458237	0.0
35	1.469245388222382E-4	3.673113470555955E-5	0.0	10.712892554819382	0.0
36	1.469245388222382E-4	3.673113470555955E-5	0.0	10.901360006993608	0.0
37	1.469245388222382E-4	3.673113470555955E-5	0.0	11.099891790077157	0.0
38	1.469245388222382E-4	3.673113470555955E-5	0.0	11.347459637992628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17215	0.0	42.261982	1
GTTTTTA	9145	0.0	40.487698	2
AGCGATA	595	0.0	39.932774	8
GAGCGAT	2690	0.0	39.74721	7
CTATCTC	1470	0.0	39.210884	6
CGCATGA	175	0.0	37.714287	2
GTTTACG	85	0.0	36.23529	1
TATCTCT	1615	0.0	35.826622	7
TATTTTT	10770	0.0	35.62488	7
TTATTTT	10805	0.0	35.61129	6
GACCGAT	1905	0.0	35.454067	9
AGACCGA	1075	0.0	35.2	8
AGCCGAT	295	0.0	35.05085	8
TATACTA	1470	0.0	34.57143	44
ATGTCTC	2400	0.0	34.558334	8
CTAATCC	1710	0.0	34.47953	11
AGCGATG	1210	0.0	34.363636	8
TTTATTT	11260	0.0	34.30906	5
GTTACGA	200	0.0	34.100002	2
GAGACCG	1245	0.0	33.92771	7
>>END_MODULE