FastQCFastQC Report
Thu 26 May 2016
SRR1546719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546719_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3077451
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218620.7103931143014137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT320300.043.1208231
GAGCGAT28200.042.127667
CGTACGA850.041.4117622
GTTTTTA180200.039.6659242
TATGCGA2200.039.0000042
AGCGATA7250.037.9310348
CTATCTC16650.036.2042056
CGAGACT4700.036.0425536
AGCGATC3850.036.08
AGACCGA9600.035.9791648
GACCGAT18700.035.8823559
GCGAGGT5100.035.803925
AGCGATG13400.035.7910428
TTATTTT204700.035.5417676
TATTTTT204500.035.522747
TTTGCGA3100.035.483872
GCGATAA4450.034.6067439
CGAGATT7450.034.5503356
TTTTATT212900.034.4208564
ATGTCTC23550.034.3779228
Produced by FastQC (version 0.11.5)