##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546718_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3544840
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53365709030591	31.0	31.0	34.0	30.0	34.0
2	31.913453639656513	31.0	31.0	34.0	30.0	34.0
3	31.98139746786879	33.0	31.0	34.0	30.0	34.0
4	35.70146268943027	37.0	35.0	37.0	33.0	37.0
5	35.65162179393146	37.0	35.0	37.0	33.0	37.0
6	35.68829227835389	37.0	35.0	37.0	33.0	37.0
7	36.06357663533474	37.0	35.0	37.0	35.0	37.0
8	18.286990950226244	27.0	0.0	37.0	0.0	37.0
9	27.82711067354239	26.0	17.0	39.0	17.0	39.0
10	33.5258510962413	32.0	32.0	39.0	27.0	39.0
11	35.75995757213302	37.0	35.0	39.0	32.0	39.0
12	36.70021213933492	38.0	35.0	39.0	33.0	39.0
13	37.03300064318841	39.0	37.0	39.0	33.0	39.0
14	38.25612298439422	40.0	37.0	41.0	33.0	41.0
15	38.39816945193577	40.0	38.0	41.0	34.0	41.0
16	38.41157880186412	40.0	38.0	41.0	34.0	41.0
17	38.37090841899776	40.0	38.0	41.0	33.0	41.0
18	38.2951224878979	40.0	37.0	41.0	33.0	41.0
19	38.26615079947191	40.0	37.0	41.0	34.0	41.0
20	38.14724359914693	40.0	37.0	41.0	34.0	41.0
21	38.04605087958836	40.0	36.0	41.0	33.0	41.0
22	38.0298555082881	40.0	36.0	41.0	33.0	41.0
23	37.809235113573536	39.0	36.0	41.0	33.0	41.0
24	37.580227598424756	39.0	35.0	41.0	33.0	41.0
25	37.437194344455605	39.0	35.0	41.0	33.0	41.0
26	37.4142624208709	39.0	35.0	41.0	33.0	41.0
27	37.290311551438144	39.0	35.0	41.0	33.0	41.0
28	37.25010578756728	39.0	35.0	41.0	33.0	41.0
29	37.14007966509067	39.0	35.0	41.0	32.0	41.0
30	36.99286794326401	39.0	35.0	41.0	32.0	41.0
31	36.90898827591654	39.0	35.0	41.0	31.0	41.0
32	36.5240433982916	39.0	35.0	41.0	30.0	41.0
33	36.14269981155708	39.0	35.0	41.0	29.0	41.0
34	35.78710830390088	39.0	35.0	41.0	25.0	41.0
35	35.453592263684676	39.0	35.0	41.0	23.0	41.0
36	35.29320674557949	39.0	35.0	41.0	23.0	41.0
37	35.227866137822865	39.0	35.0	41.0	22.0	41.0
38	35.15275216935038	39.0	35.0	41.0	22.0	41.0
39	35.082292853838254	39.0	35.0	41.0	21.0	41.0
40	35.045078480269915	39.0	35.0	41.0	21.0	41.0
41	35.0292388372959	39.0	35.0	41.0	21.0	41.0
42	34.869487480394035	39.0	35.0	40.0	20.0	41.0
43	34.8895146184313	39.0	35.0	40.0	20.0	41.0
44	34.65363542501213	38.0	34.0	40.0	18.0	41.0
45	34.56736129134178	38.0	34.0	40.0	19.0	41.0
46	34.575846300538245	38.0	34.0	40.0	20.0	41.0
47	34.46248744654201	38.0	34.0	40.0	19.0	41.0
48	34.42951501337155	38.0	34.0	40.0	19.0	41.0
49	34.39253562925266	38.0	34.0	40.0	19.0	41.0
50	33.815963484952775	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	5.0
12	1.0
13	8.0
14	14.0
15	50.0
16	142.0
17	367.0
18	1099.0
19	2524.0
20	5133.0
21	8698.0
22	14256.0
23	21765.0
24	33892.0
25	54675.0
26	77278.0
27	84233.0
28	78665.0
29	74750.0
30	82014.0
31	97702.0
32	123238.0
33	159683.0
34	220498.0
35	304554.0
36	359823.0
37	546889.0
38	605773.0
39	587107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.918867988400038	23.070434772796517	26.521732997822184	20.488964240981257
2	35.94128366865642	23.957668047076876	27.439658771622977	12.661389512643732
3	26.31247108473161	23.99916498346893	36.86973177914941	12.81863215265005
4	22.833103891854076	26.622188871712122	36.650032159420455	13.89467507701335
5	21.297096624953454	30.072133015876595	35.42205007842385	13.208720280746098
6	19.012480111937354	38.335580731429346	32.336748626172124	10.315190530461177
7	81.12639780638901	2.835445323343226	14.021874047911895	2.016282822355875
8	41.242199930039156	50.51934643030434	6.741432617551145	1.4970210221053701
9	74.88022026382009	5.288927003757574	16.14298529693865	3.687867435483689
10	30.424250459823295	34.36741855767821	22.311726340257955	12.896604642240552
11	22.77609144559416	25.28760113291432	36.040244411595445	15.896063009896075
12	21.171477415059638	22.755413502442988	37.17239706164453	18.900712020852843
13	20.85357872287607	24.068561627605195	38.38404554225296	16.693814107265773
14	19.04356755170896	26.714943410704013	36.246939213053345	17.99454982453369
15	18.480918743864322	24.80645106690288	39.927810564087515	16.784819625145282
16	19.982537998894166	24.362594644610194	38.32491734464743	17.329950011848208
17	20.000112840071765	24.84952776429966	35.72101984856862	19.42933954705995
18	21.026985703162907	23.9060437142438	36.65113235012017	18.415838232473117
19	22.055184437097303	26.706565035375363	33.61186964714909	17.62638088037824
20	23.71122533033931	24.689887272768303	33.409011408131256	18.189875988761127
21	22.508970785705422	26.413406528926554	33.68961645659606	17.388006228771964
22	21.241635729680326	24.313565633427743	34.23059997066158	20.214198666230352
23	19.041451800363348	27.15569673102312	34.06729217679783	19.73555929181571
24	18.90200968167816	25.071484185463945	36.852015887882104	19.174490244975793
25	19.341719231333432	26.974137055551168	34.7109883661886	18.973155346926802
26	19.27173017682039	27.088302998160707	34.6243271910721	19.015639633946808
27	19.589741709075728	25.65365432572415	34.568414935511896	20.18818902968822
28	19.14492614617303	26.467174823123184	35.92449870798118	18.463400322722606
29	19.408746233962603	25.289519414134347	34.71598153936426	20.58575281253879
30	19.827749630448764	26.09203800453617	34.456308324212095	19.62390404080297
31	20.46030286275262	25.961284571376986	32.50403403256564	21.074378533304746
32	20.39011633811399	27.697413705555118	32.171861071303645	19.74060888502725
33	19.836692206136245	27.737471931032147	31.48051816160955	20.94531770122206
34	18.52577267239142	28.717149434107043	31.97137247379289	20.78570541970865
35	18.97707653942068	28.484219315963482	30.833803500299027	21.70490064431681
36	19.647346565712414	29.01403166292414	30.060425858430843	21.278195912932603
37	19.525902438473953	28.71534399295878	29.79897541214836	21.959778156418906
38	19.194237257534898	28.44255311946378	30.507977793073877	21.855231829927444
39	19.704246171900568	27.574333397276042	29.275030748919555	23.446389681903838
40	20.81794947021586	26.52596450051342	30.547782128389432	22.108303900881282
41	18.945565949379944	26.724929757055328	30.63819523589217	23.691309057672562
42	20.663443201949878	27.740603243023664	29.454023312758824	22.141930242267634
43	20.35976235880886	26.712348089053386	31.074181063179157	21.8537084889586
44	20.877275137946988	28.21162591259408	30.102769095361143	20.80832985409779
45	19.911110233466108	27.53681407341375	30.49009264169892	22.061983051421223
46	21.163550400018057	27.411505173717295	29.82789068053847	21.597053745726182
47	20.542196544837	26.59522009455998	30.6836133647781	22.178969995824918
48	20.569926992473565	25.95378070660453	30.870194423443653	22.606097877478252
49	20.65114363412735	25.535623610656614	30.815890138906127	22.997342616309904
50	19.49357375791291	26.328014804617418	32.24732286929735	21.93108856817233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1003.0
1	1901.5
2	2800.0
3	42626.5
4	82453.0
5	59428.5
6	36404.0
7	36667.0
8	36930.0
9	37267.5
10	37605.0
11	37451.0
12	37297.0
13	36209.0
14	35121.0
15	33836.5
16	32552.0
17	31141.5
18	29731.0
19	29233.0
20	28735.0
21	29330.0
22	29925.0
23	31289.5
24	32654.0
25	37035.0
26	41416.0
27	48324.0
28	55232.0
29	63478.5
30	71725.0
31	80790.5
32	89856.0
33	100895.0
34	111934.0
35	124561.5
36	137189.0
37	149305.0
38	161421.0
39	177518.0
40	193615.0
41	209871.0
42	226127.0
43	243498.5
44	260870.0
45	282577.5
46	304285.0
47	314403.5
48	324522.0
49	307850.5
50	291179.0
51	255814.5
52	220450.0
53	194156.0
54	167862.0
55	149541.0
56	131220.0
57	117996.5
58	104773.0
59	92553.5
60	80334.0
61	68595.0
62	56856.0
63	46774.0
64	36692.0
65	28934.5
66	21177.0
67	17125.5
68	13074.0
69	10616.0
70	8158.0
71	6569.5
72	4981.0
73	4066.0
74	3151.0
75	2500.5
76	1850.0
77	1432.0
78	1014.0
79	707.5
80	401.0
81	278.5
82	156.0
83	110.5
84	65.0
85	42.5
86	20.0
87	17.0
88	14.0
89	10.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3544840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.405150907422176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61352675129828	29.743656572137667
2	7.694274677352496	6.217766589231694
3	3.7943190498658232	4.59930101402206
4	2.578149127953221	4.166820183071551
5	1.933857090757364	3.9068893792719863
6	1.6200964730937035	3.9276145487960115
7	1.2944282511967946	3.6611098179905945
8	1.0809928306787553	3.494214276273326
9	0.9318674454031293	3.388702028150469
>10	5.412029089693666	33.77782899299382
>50	0.035688205701127226	0.9595200160054083
>100	0.009162047814396992	0.6520771277719687
>500	8.849275553421531E-4	0.2585457202388533
>1k	6.435836764492722E-4	0.4630354267163612
>5k	0.0	0.0
>10k+	8.044795955615903E-5	0.7829183073282047
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24086	0.679466492140689	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07816995971609438	0.0
2	0.0	0.0	0.0	0.1978368558242403	0.0
3	0.0	0.0	0.0	0.3363762539352975	0.0
4	0.0	0.0	0.0	0.477398133625213	0.0
5	0.0	0.0	0.0	0.7517687681249365	0.0
6	0.0	0.0	0.0	1.366098328838537	0.0
7	0.0	0.0	0.0	1.7352546236219406	0.0
8	1.9747012559099989E-4	0.0	0.0	2.557379176493156	0.0
9	1.9747012559099989E-4	0.0	0.0	3.4717504880333103	0.0
10	1.9747012559099989E-4	0.0	0.0	4.608670631114522	0.0
11	2.256801435325713E-4	0.0	0.0	5.210531363897948	0.0
12	2.256801435325713E-4	0.0	0.0	5.824748084539782	0.0
13	2.256801435325713E-4	0.0	0.0	6.231000552916352	0.0
14	2.256801435325713E-4	0.0	0.0	6.479953961250719	0.0
15	2.256801435325713E-4	0.0	0.0	6.658297694677334	0.0
16	2.256801435325713E-4	0.0	0.0	6.907166472957877	0.0
17	2.256801435325713E-4	0.0	0.0	7.168250189007121	0.0
18	2.256801435325713E-4	0.0	0.0	7.582824612676454	0.0
19	2.256801435325713E-4	0.0	0.0	7.7903939246905365	0.0
20	2.256801435325713E-4	0.0	0.0	8.015340607756627	0.0
21	2.256801435325713E-4	0.0	0.0	8.232727006014375	0.0
22	2.256801435325713E-4	0.0	0.0	8.46997325690299	0.0
23	2.256801435325713E-4	0.0	0.0	8.736783606594374	0.0
24	2.256801435325713E-4	0.0	0.0	8.961250719355457	0.0
25	2.256801435325713E-4	0.0	0.0	9.169017501495132	0.0
26	2.256801435325713E-4	0.0	0.0	9.366487627086132	0.0
27	2.538901614741427E-4	0.0	0.0	9.58548199636655	0.0
28	2.538901614741427E-4	0.0	0.0	9.790455986730008	0.0
29	2.538901614741427E-4	0.0	0.0	10.002482481578859	0.0
30	2.538901614741427E-4	0.0	0.0	10.267797700319337	0.0
31	2.538901614741427E-4	0.0	0.0	10.493562473905733	0.0
32	2.538901614741427E-4	0.0	0.0	10.744377743424245	0.0
33	2.538901614741427E-4	0.0	0.0	10.97208900824861	0.0
34	3.103101973572855E-4	0.0	0.0	11.200025953216507	0.0
35	3.103101973572855E-4	0.0	0.0	11.437526094266596	0.0
36	3.103101973572855E-4	0.0	0.0	11.670117692194852	0.0
37	3.3852021529885695E-4	0.0	0.0	11.909564324482911	0.0
38	3.3852021529885695E-4	0.0	0.0	12.160520644091129	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	37750	0.0	43.07338	1
GAGCGAT	3845	0.0	42.283485	7
GTTTTTA	20305	0.0	40.597885	2
AGCGATA	770	0.0	39.42857	8
ATCGCGA	135	0.0	39.111115	2
CGAGATT	860	0.0	38.88372	6
TATGCGA	170	0.0	38.823532	2
AGCGATC	545	0.0	38.752293	8
GAGACCG	1315	0.0	38.64639	7
CTATCTC	2005	0.0	37.52618	6
AGCGATT	1205	0.0	37.244816	8
AGACCGA	1265	0.0	37.04348	8
AGTACGA	230	0.0	36.347824	2
AGCGATG	1910	0.0	36.282722	8
TATTTTT	23200	0.0	36.025	7
GACCGAT	2330	0.0	35.785408	9
TATCGCG	105	0.0	35.61905	1
AGAGCGA	2465	0.0	35.43205	6
TTATTTT	23630	0.0	35.388065	6
GCGTAAG	255	0.0	35.37255	1
>>END_MODULE