FastQCFastQC Report
Thu 26 May 2016
SRR1546717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546717_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1491946
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58500.3921053442953029No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53300.35725153591349823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA351.4464786E-744.02
TATAGCG700.040.8571431
GTAACGA650.040.6153872
CGTTTTT63100.040.1996841
GAGCGAT16600.039.8915677
GTTTTTA35750.038.769232
TACGAAT1150.038.2608712
AGCGATA4250.037.7882358
CTATGCG357.291668E-637.7142871
CGTTCGA601.9826984E-1036.66666814
ATATCGG301.301281E-436.6666681
TAGTACG301.301281E-436.6666681
TCGATAG1400.036.1428571
CGGATCG250.002352969335.217
GCCGATT1650.034.6666689
TGATTCG3050.034.6229515
AGACCGA5600.034.571438
GACCGAT11350.034.5022059
TAGCATA6400.034.37530
TAGAGGC6550.034.259545
Produced by FastQC (version 0.11.5)