FastQCFastQC Report
Thu 26 May 2016
SRR1546716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546716_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1058079
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47800.451762108500405No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.4198174238407529No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA254.442599E-544.02
CGCATCG207.856198E-444.021
TAACGCG207.856198E-444.01
GCGTTAG800.044.01
TATACGA454.802132E-1044.02
GCCGTAA207.856198E-444.037
GAGCGAT11050.043.601817
CGTTTTT53400.042.3520621
GTATGCG501.3478711E-939.6000021
TAGTACG501.3478711E-939.6000021
GTTTTTA30100.038.5182722
AGCGATC1500.038.133338
TCGTCCC1850.038.05405438
CGTTAGA1050.037.7142872
GAGACCG3500.037.7142877
AGACCGA3150.037.7142878
AGCGATG5250.037.7142878
AGCGATA2750.037.68
GACGTAG301.3010256E-436.6666641
TCGGGTA301.3010256E-436.6666645