FastQCFastQC Report
Thu 26 May 2016
SRR1546715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546715_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1615791
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81840.5065011502106398No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.4701103051075294No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG207.857431E-444.01
CGTTTTT110500.042.2280541
TAAGCGA1100.042.02
GAGCGAT18000.041.188897
CGTTAGA850.038.8235322
ACGAGAC2100.038.7619065
TATCGCG404.1259227E-738.51
TCGTAGA750.038.133332
GTTTTTA62100.038.0128822
AGCGATA4300.037.8604668
AGCCGAT2600.037.2307668
AGAGCGA10050.036.776126
CTATACG601.9826984E-1036.6666641
GTTAGCG601.9826984E-1036.6666641
AGCGATG8300.036.313258
AGACCGA5700.036.28078
GTTACGA850.036.2352942
GACCGAT10850.035.88949
ATTGCGA1350.035.8518522
TAGAGAT15650.035.8466455
Produced by FastQC (version 0.11.5)