##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546715_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1615791
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57098411861435	31.0	31.0	34.0	30.0	34.0
2	31.947916531284058	31.0	31.0	34.0	30.0	34.0
3	32.033294528809726	33.0	31.0	34.0	30.0	34.0
4	35.73448731921393	37.0	35.0	37.0	35.0	37.0
5	35.67873753474304	37.0	35.0	37.0	33.0	37.0
6	35.71615388376343	37.0	35.0	37.0	33.0	37.0
7	36.07432273109579	37.0	35.0	37.0	35.0	37.0
8	18.48266143331656	30.0	0.0	37.0	0.0	37.0
9	27.935307227234215	30.0	17.0	39.0	17.0	39.0
10	33.65507110758755	32.0	32.0	39.0	27.0	39.0
11	35.843545359517414	37.0	35.0	39.0	32.0	39.0
12	36.68095316782926	38.0	35.0	39.0	33.0	39.0
13	36.957107076348365	39.0	37.0	39.0	33.0	39.0
14	38.15451193873465	40.0	37.0	41.0	33.0	41.0
15	38.31005618919774	40.0	37.0	41.0	33.0	41.0
16	38.346093647012516	40.0	37.0	41.0	34.0	41.0
17	38.288781779326655	40.0	37.0	41.0	33.0	41.0
18	38.24562025658021	40.0	37.0	41.0	33.0	41.0
19	38.23850361835163	40.0	37.0	41.0	34.0	41.0
20	38.15554053711154	40.0	37.0	41.0	34.0	41.0
21	38.028300689878826	40.0	36.0	41.0	33.0	41.0
22	37.999907785103396	40.0	36.0	41.0	33.0	41.0
23	37.82283228462097	39.0	36.0	41.0	33.0	41.0
24	37.645534601937996	39.0	35.0	41.0	33.0	41.0
25	37.50440620104952	39.0	35.0	41.0	33.0	41.0
26	37.472322224842195	39.0	35.0	41.0	33.0	41.0
27	37.35621129217826	39.0	35.0	41.0	33.0	41.0
28	37.32449927001698	39.0	35.0	41.0	33.0	41.0
29	37.23918254279174	39.0	35.0	41.0	32.0	41.0
30	37.11732396083404	39.0	35.0	41.0	32.0	41.0
31	37.121555324915164	39.0	35.0	41.0	32.0	41.0
32	36.87271992479225	39.0	35.0	41.0	31.0	41.0
33	36.670573112487936	39.0	35.0	41.0	30.0	41.0
34	36.44313404394504	39.0	35.0	41.0	30.0	41.0
35	36.215186246241004	39.0	35.0	41.0	29.0	41.0
36	36.06362518419771	39.0	35.0	41.0	28.0	41.0
37	36.012146372891046	39.0	35.0	41.0	28.0	41.0
38	35.92088766430807	39.0	35.0	41.0	27.0	41.0
39	35.84781633268164	39.0	35.0	41.0	27.0	41.0
40	35.76908337773883	39.0	35.0	41.0	27.0	41.0
41	35.741215293314546	39.0	35.0	41.0	27.0	41.0
42	35.58415847099037	39.0	35.0	40.0	26.0	41.0
43	35.58383788497399	39.0	35.0	40.0	26.0	41.0
44	35.34685055183498	38.0	35.0	40.0	25.0	41.0
45	35.25562216895626	38.0	35.0	40.0	24.0	41.0
46	35.23539121086824	38.0	35.0	40.0	24.0	41.0
47	35.13414544331538	38.0	34.0	40.0	24.0	41.0
48	35.09063548441599	38.0	34.0	40.0	24.0	41.0
49	35.042625562340675	38.0	34.0	40.0	24.0	41.0
50	34.446956320464714	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	7.0
14	13.0
15	17.0
16	59.0
17	185.0
18	416.0
19	874.0
20	1873.0
21	3071.0
22	4885.0
23	7578.0
24	11644.0
25	18060.0
26	25160.0
27	28936.0
28	28913.0
29	30569.0
30	35631.0
31	44177.0
32	56845.0
33	75374.0
34	108713.0
35	154203.0
36	172836.0
37	258322.0
38	283898.0
39	263524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.155947767997223	24.19310418240973	27.485299769586536	17.16564828000651
2	33.96429364936431	24.828582409482415	28.071452310354495	13.135671630798784
3	27.8535404640823	25.385708919037175	33.88092890726585	12.879821709614673
4	24.843807150801062	27.49872972432697	33.35010530446078	14.307357820411179
5	22.66109911492266	30.912908909630023	32.22279366576494	14.203198309682378
6	20.806837022857536	39.26058506329098	29.203529416861464	10.72904849699002
7	84.41673459005527	3.45279804133084	10.150322659304328	1.9801447093095579
8	43.45927165085088	50.203832055012064	4.867151754156324	1.4697445399807278
9	78.3318510871765	5.657167294532523	12.205043845398322	3.8059377728926576
10	34.30103274495278	35.6969434784573	16.986540957339162	13.015482819250757
11	28.155745390338232	25.08882646332354	31.397377507363267	15.358050638974966
12	26.00169205051891	23.238587168761306	32.29935059670465	18.460370184015133
13	22.376470719294762	25.665262400892196	34.17038465989723	17.78788221991582
14	19.77811486757879	28.23811990535905	33.22409890883165	18.759666318230515
15	19.16200795771235	24.849500956497465	38.06637120766238	17.922119878127802
16	21.584536613955642	23.87270383360224	36.55553224395977	17.98722730848235
17	21.034898696675498	24.098475607303172	32.91032070360585	21.95630499241548
18	22.755294465682752	23.786987302194404	33.638447051629825	19.819271180493022
19	24.69323074580809	26.277284624063384	30.412844235424014	18.616640394704515
20	27.243436805874026	24.800979829693322	29.19368903527746	18.761894329155194
21	24.924324990051314	26.600965100065544	30.0168152935621	18.457894616321045
22	23.255482918273465	24.49301920854863	30.70378532867184	21.547712544506066
23	21.58001870291393	28.06241648827107	29.97745376722608	20.38011104158892
24	21.060087597962855	25.21761787260852	33.76742412849186	19.954870400936755
25	20.9697293771286	26.399206332997277	31.740800635725787	20.890263654148338
26	20.248348951071023	28.301308770750673	31.21975552531237	20.230586752865936
27	21.291119953013727	26.234890527302106	31.450540323593827	21.023449196090336
28	20.1255607934442	27.219733245203127	33.32516395994284	19.329542001409834
29	20.950915062653525	25.405265903820485	32.50723639381579	21.136582639710213
30	21.750152092690207	25.24218788197236	32.30553951593987	20.702120509397563
31	23.710677928024108	25.199484339249324	29.54181574225874	21.548021990467827
32	23.89993507823722	26.21861366971347	30.3750299388968	19.506421313152504
33	22.682079551130066	26.27753218083279	29.762326934609735	21.278061333427406
34	21.32683001700096	27.54626062405348	30.587804982203764	20.539104376741793
35	21.03130912351907	27.031528211259996	30.580873392660312	21.356289272560623
36	23.01869486833384	29.06656863418598	27.825999773485556	20.08873672399463
37	21.81853965023942	28.478868863609218	28.3354716049291	21.367119881222262
38	21.988734929208047	29.492923280300488	27.537719915508873	20.980621874982592
39	22.07847425811878	27.791341825768306	27.54489906182173	22.585284854291178
40	22.823063131308444	26.813616364987798	28.88350040320809	21.47982010049567
41	19.926463261647083	26.862632605330766	29.153646727825567	24.057257405196587
42	22.46206347231789	27.671957573720857	27.444638570211122	22.421340383750128
43	21.94299881605975	26.601026989257893	28.92744172977817	22.528532464904185
44	21.875972820742287	28.12071610746687	28.759474461734218	21.243836610056622
45	21.138315537096073	27.524042403999033	28.640213988071476	22.69742807083342
46	22.15713542159846	27.655990162093985	28.335719161698513	21.851155254609044
47	22.04969578367499	26.50627463576663	29.238868145694585	22.20516143486379
48	22.444672609266917	25.25227582032577	29.6600240996515	22.643027470755808
49	22.018936855075935	25.464060636555097	29.500226204998047	23.01677630337092
50	20.618260653760295	26.596694745793236	30.476837660316214	22.30820694013025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	484.0
1	953.5
2	1423.0
3	14301.5
4	27180.0
5	19350.5
6	11521.0
7	11354.5
8	11188.0
9	11436.0
10	11684.0
11	11675.5
12	11667.0
13	11406.0
14	11145.0
15	10760.0
16	10375.0
17	10217.0
18	10059.0
19	9927.0
20	9795.0
21	10394.0
22	10993.0
23	12197.5
24	13402.0
25	16016.0
26	18630.0
27	21418.5
28	24207.0
29	28414.0
30	32621.0
31	36593.0
32	40565.0
33	44389.5
34	48214.0
35	53325.5
36	58437.0
37	62961.5
38	67486.0
39	75164.5
40	82843.0
41	91563.5
42	100284.0
43	107459.0
44	114634.0
45	121733.0
46	128832.0
47	132546.0
48	136260.0
49	130234.5
50	124209.0
51	112037.5
52	99866.0
53	90619.0
54	81372.0
55	74915.5
56	68459.0
57	65344.0
58	62229.0
59	58375.5
60	54522.0
61	48604.5
62	42687.0
63	36218.5
64	29750.0
65	24688.5
66	19627.0
67	16413.0
68	13199.0
69	11223.0
70	9247.0
71	7942.0
72	6637.0
73	5646.5
74	4656.0
75	3794.0
76	2932.0
77	2206.5
78	1481.0
79	1030.0
80	579.0
81	408.5
82	238.0
83	176.5
84	115.0
85	76.0
86	37.0
87	24.5
88	12.0
89	7.5
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1615791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.20388070071097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00313346018552	24.981812117677933
2	7.724839489368872	5.593383346105033
3	4.361612197384539	4.73721862970627
4	3.1288477135679553	4.531057174108262
5	2.5150033248067425	4.552644016659737
6	2.0638604986954983	4.483185556660899
7	1.7269026581690332	4.376440447266463
8	1.50613094443265	4.362222802551104
9	1.2888061273145361	4.19938049516767
>10	6.63385382639904	35.20045013851642
>50	0.030483619217817448	0.7237416821963718
>100	0.015448367662388472	1.0418337840201484
>500	7.185151969659646E-4	0.19041544161945853
>1k	0.0	0.0
>5k	3.592575984829823E-4	1.026214367744213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8184	0.5065011502106398	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7596	0.4701103051075294	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03688595864192832	0.0
2	0.0	0.0	0.0	0.10892497853992256	0.0
3	0.0	0.0	0.0	0.19470339914011156	0.0
4	0.0	0.0	0.0	0.3031951533335685	0.0
5	0.0	0.0	0.0	0.5069962637494577	0.0
6	0.0	0.0	0.0	0.9816244798986997	0.0
7	0.0	0.0	0.0	1.256350604750243	0.0
8	0.0	0.0	0.0	1.9746365711902096	0.0
9	0.0	0.0	0.0	2.7763491689209805	0.0
10	6.188919235222873E-5	0.0	0.0	3.7489378267362548	0.0
11	6.188919235222873E-5	0.0	0.0	4.25364419036868	0.0
12	6.188919235222873E-5	0.0	0.0	4.723568827899153	0.0
13	6.188919235222873E-5	0.0	0.0	5.006154880179429	0.0
14	6.188919235222873E-5	0.0	0.0	5.161991866522341	0.0
15	1.2377838470445745E-4	0.0	0.0	5.28385168626388	0.0
16	1.2377838470445745E-4	0.0	0.0	5.4575746491965855	0.0
17	1.2377838470445745E-4	0.0	0.0	5.644170564138555	0.0
18	1.2377838470445745E-4	0.0	0.0	5.927746843496467	0.0
19	1.2377838470445745E-4	0.0	0.0	6.0775805781812124	0.0
20	1.2377838470445745E-4	0.0	0.0	6.245424067840457	0.0
21	1.2377838470445745E-4	0.0	0.0	6.41933269835022	0.0
22	1.2377838470445745E-4	0.0	0.0	6.606052391676894	0.0
23	1.2377838470445745E-4	0.0	0.0	6.809544056131022	0.0
24	1.2377838470445745E-4	0.0	0.0	6.967485275013909	0.0
25	1.2377838470445745E-4	0.0	0.0	7.119732688200393	0.0
26	1.2377838470445745E-4	0.0	0.0	7.260901935955826	0.0
27	1.2377838470445745E-4	0.0	0.0	7.410116778717049	0.0
28	1.2377838470445745E-4	0.0	0.0	7.5650873163670305	0.0
29	1.2377838470445745E-4	0.0	0.0	7.727608335483983	0.0
30	1.2377838470445745E-4	0.0	0.0	7.934751462286893	0.0
31	1.2377838470445745E-4	0.0	0.0	8.117077022956558	0.0
32	1.2377838470445745E-4	0.0	0.0	8.327128941800023	0.0
33	1.2377838470445745E-4	0.0	0.0	8.507164602352656	0.0
34	1.2377838470445745E-4	0.0	0.0	8.677421770513638	0.0
35	1.2377838470445745E-4	0.0	0.0	8.858200101374496	0.0
36	1.2377838470445745E-4	0.0	0.0	9.042011002660617	0.0
37	1.2377838470445745E-4	0.0	0.0	9.234053166529582	0.0
38	1.2377838470445745E-4	0.0	0.0	9.450294004608269	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAACG	20	7.857431E-4	44.0	1
CGTTTTT	11050	0.0	42.228054	1
TAAGCGA	110	0.0	42.0	2
GAGCGAT	1800	0.0	41.18889	7
CGTTAGA	85	0.0	38.823532	2
ACGAGAC	210	0.0	38.761906	5
TATCGCG	40	4.1259227E-7	38.5	1
TCGTAGA	75	0.0	38.13333	2
GTTTTTA	6210	0.0	38.012882	2
AGCGATA	430	0.0	37.860466	8
AGCCGAT	260	0.0	37.230766	8
AGAGCGA	1005	0.0	36.77612	6
CTATACG	60	1.9826984E-10	36.666664	1
GTTAGCG	60	1.9826984E-10	36.666664	1
AGCGATG	830	0.0	36.31325	8
AGACCGA	570	0.0	36.2807	8
GTTACGA	85	0.0	36.235294	2
GACCGAT	1085	0.0	35.8894	9
ATTGCGA	135	0.0	35.851852	2
TAGAGAT	1565	0.0	35.846645	5
>>END_MODULE