FastQCFastQC Report
Thu 26 May 2016
SRR1546714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546714_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1650542
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95630.5793854382378637No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89810.5441242937168518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA750.044.02
CGTTTTT160600.042.9726031
GAGCGAT17100.042.4561427
TGTGCGA1350.040.740742
AGCGATC2450.040.408168
GTTTTTA89700.039.9041252
AGACCGA4450.039.5505648
TACGCGA452.3519533E-839.111112
TTAGCGA1200.038.52
AGCGATA4950.038.222228
TATTGCG1450.037.9310381
TAGCGAG3950.037.8734173
CGCACGA357.2921375E-637.7142872
ATCGCGA357.2921375E-637.7142872
AGCGATG7450.037.2080548
TGTAGCG1250.036.961
TCGTTAG900.036.6666681
TCGTAGA1200.036.6666642
TTACGCG301.3013411E-436.6666641
GCGATCG1450.036.4137959
Produced by FastQC (version 0.11.5)