FastQCFastQC Report
Thu 26 May 2016
SRR1546713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546713_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1099694
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.3457325401429852No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.3310921038034217No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12390.11266770574359776No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACT207.856332E-444.00000429
CATCGTA207.856332E-444.00000427
GTTTCGA254.442713E-544.02
TCGTAAG254.442713E-544.01
GTATACG302.5276167E-644.01
CGTTTTT51250.042.0253681
GAGCGAT10750.040.520937
AACCGAT501.3478711E-939.6000028
CTATCTC6100.039.3114746
TATCGCG452.3508619E-839.111111
GTTTTTA28500.038.9052662
GCGATAT1250.038.729
GTTACGA404.124322E-738.5000042
TATTACG404.124322E-738.5000041
AGCGATA2650.037.358498
TCGATAG651.0913936E-1137.230771
ATGTCTC9550.037.0890058
CGTAAGA900.036.6666682
AACGTTG301.3010588E-436.6666641
AACGTCG301.3010588E-436.66666427
Produced by FastQC (version 0.11.5)