FastQCFastQC Report
Thu 26 May 2016
SRR1546712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546712_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1755099
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60860.3467610658999863No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56900.32419823611089743No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21940.12500719332641633No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG254.443802E-544.01
CGTAAGA1150.044.02
TGATTCG1600.042.62500415
ATTGCGA1500.042.5333332
AGCGATA3850.040.5714268
CGTTAGG1200.040.3333322
CGTTTTT62500.040.2688031
TATAGCG557.8216544E-1140.01
CGCATCG452.3521352E-839.1111121
GAGCGAT19250.039.0857127
TATGCGA1300.038.9230772
CTATCTC8600.038.8837246
GTTTTTA34750.038.048922
CCGTTTA357.2924013E-637.71428713
TCGTAAG651.0913936E-1137.2307661
TATCACG601.9826984E-1036.6666641
ATTACCG301.3013753E-436.6666641
ATTCGGC1900.035.89473717
GCGTTAG1900.035.8947371
AACCGTT4050.035.85185244
Produced by FastQC (version 0.11.5)