##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546712_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1755099
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46854735829717	31.0	31.0	33.0	30.0	34.0
2	31.852237395155488	31.0	31.0	34.0	30.0	34.0
3	31.98165915426993	31.0	31.0	34.0	30.0	34.0
4	35.6805456558291	37.0	35.0	37.0	33.0	37.0
5	35.61837651323373	37.0	35.0	37.0	33.0	37.0
6	35.65241675825694	37.0	35.0	37.0	33.0	37.0
7	35.992169102711586	37.0	35.0	37.0	35.0	37.0
8	18.645925956313576	32.0	0.0	37.0	0.0	37.0
9	28.01013162220479	31.0	17.0	39.0	17.0	39.0
10	33.59385481958567	32.0	32.0	39.0	27.0	39.0
11	35.73737492870772	37.0	35.0	39.0	32.0	39.0
12	36.40828010271785	37.0	35.0	39.0	32.0	39.0
13	36.61003225459077	39.0	35.0	39.0	33.0	39.0
14	37.68095702863485	39.0	36.0	41.0	33.0	41.0
15	37.919269511292526	40.0	36.0	41.0	33.0	41.0
16	37.99959774348911	40.0	36.0	41.0	33.0	41.0
17	37.958524846746535	40.0	36.0	41.0	33.0	41.0
18	37.95301119765894	40.0	36.0	41.0	33.0	41.0
19	37.952052847161326	40.0	36.0	41.0	33.0	41.0
20	37.86613575644451	40.0	36.0	41.0	33.0	41.0
21	37.712320501578546	40.0	36.0	41.0	33.0	41.0
22	37.720022631202	40.0	36.0	41.0	33.0	41.0
23	37.57437557653443	39.0	36.0	41.0	33.0	41.0
24	37.43159388729639	39.0	35.0	41.0	32.0	41.0
25	37.263825573372216	39.0	35.0	41.0	32.0	41.0
26	37.242841002131506	39.0	35.0	41.0	32.0	41.0
27	37.0994593467377	39.0	35.0	41.0	32.0	41.0
28	37.109129456514985	39.0	35.0	41.0	32.0	41.0
29	37.00155660734808	39.0	35.0	41.0	32.0	41.0
30	36.87288295418093	39.0	35.0	40.0	31.0	41.0
31	36.879491698189106	39.0	35.0	41.0	31.0	41.0
32	36.701847588084775	39.0	35.0	41.0	31.0	41.0
33	36.63139002415248	39.0	35.0	41.0	31.0	41.0
34	36.49267933033977	39.0	35.0	41.0	30.0	41.0
35	36.31909539006062	39.0	35.0	41.0	30.0	41.0
36	36.178115308595125	39.0	35.0	41.0	30.0	41.0
37	36.11275774187097	39.0	35.0	40.0	30.0	41.0
38	36.002294457463655	38.0	35.0	40.0	30.0	41.0
39	35.93010080912815	38.0	35.0	40.0	29.0	41.0
40	35.83180777836464	38.0	35.0	40.0	29.0	41.0
41	35.79492324934377	38.0	35.0	40.0	29.0	41.0
42	35.62746488944498	38.0	35.0	40.0	28.0	41.0
43	35.610318278342135	38.0	35.0	40.0	28.0	41.0
44	35.33942244853424	38.0	34.0	40.0	27.0	41.0
45	35.21640830517253	37.0	34.0	40.0	27.0	41.0
46	35.193264881354274	37.0	34.0	40.0	27.0	41.0
47	35.042379375750315	37.0	34.0	40.0	27.0	41.0
48	35.00326078471927	37.0	34.0	40.0	27.0	41.0
49	34.959774348911374	37.0	34.0	40.0	27.0	41.0
50	34.355454592589936	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	3.0
12	4.0
13	9.0
14	19.0
15	29.0
16	80.0
17	225.0
18	472.0
19	1099.0
20	1965.0
21	3431.0
22	5112.0
23	7699.0
24	11022.0
25	15744.0
26	21099.0
27	25353.0
28	29044.0
29	34470.0
30	42315.0
31	53895.0
32	70158.0
33	92881.0
34	138923.0
35	199871.0
36	200470.0
37	284838.0
38	286100.0
39	228766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.191175540525066	24.89403731641349	28.65513569320021	14.259651449861233
2	30.934038478741083	25.681742169530036	29.273334438684085	14.11088491304479
3	28.57850183949737	25.624366488727983	31.519532516399362	14.277599155375281
4	25.712851525754388	28.228721000923596	30.442328324499073	15.616099148822943
5	23.438962702388867	30.86378603144324	29.88731689779323	15.809934368374662
6	21.65553054272152	39.722944403706	27.133227242451852	11.488297811120626
7	85.79288119929417	4.410406478494945	7.637688814135271	2.1590235080756126
8	44.46535494578938	49.936556285428914	3.7301599510910783	1.867928817690626
9	80.1698935501644	5.1972566789679675	10.220620033399825	4.412229737467801
10	46.15044507460833	23.771365603877616	15.54163041515037	14.536558906363686
11	36.66750422625732	23.408138230379027	23.38272655844485	16.541630984918797
12	30.8919895686796	21.019270138037797	28.118527786751628	19.97021250653097
13	23.628638612408757	27.543517488187273	29.94395187963756	18.883892019766407
14	20.130602319299367	28.854326735984692	31.69849677995372	19.316574164762216
15	19.174644849093983	21.52841520620774	39.447290437747384	19.84964950695089
16	22.856089599504074	19.853979747011422	36.06121364093992	21.228717012544593
17	23.070721366714924	21.27224732052152	28.237552411573365	27.419478901190192
18	25.572346631158698	21.20729371961354	30.66294266021461	22.557416989013156
19	28.42249924363241	24.307574672425886	25.668010750390717	21.601915333550988
20	30.010899670047102	23.31065085217415	24.79649296136571	21.881956516413034
21	26.083030074086988	26.02257764376824	26.935232713368308	20.95915956877646
22	25.91922165074449	22.161655838217673	26.26592573980157	25.653196771236264
23	23.63353862089831	26.839283709921773	25.51235001558317	24.014827653596747
24	23.39583123231225	23.574225727437597	31.425862586668902	21.60408045358125
25	23.786521444089477	23.759001628967937	29.071237576911617	23.38323935003097
26	21.990668332669554	26.356461943172437	27.24814953458466	24.40472018957335
27	22.908337364444968	24.92548853369525	28.082746329409336	24.083427772450445
28	21.484030245587284	24.9172838683174	30.84823135333107	22.75045453276425
29	22.619236863561543	23.709773636700834	29.78424578898398	23.88674371075364
30	24.718605617119035	23.977963636239323	28.55747738446663	22.745953362175012
31	28.727610237371227	23.291734540330776	25.30250430317606	22.678150919121943
32	28.499190074178156	23.013003824855463	25.608697856930007	22.879108244036374
33	27.463920838653543	22.945543242859802	25.365919529325698	24.224616389160953
34	22.82486628959392	24.062118433205193	28.67308339871426	24.439931878486625
35	23.501010484308864	24.815523226894893	29.51417555362974	22.169290735166506
36	27.00480143855133	24.538159955649224	25.521124449390037	22.93591415640941
37	23.409733581980277	26.18433490076628	27.50671044767275	22.89922106958069
38	23.50391630329685	27.74014457304118	25.5133755987554	23.242563524906572
39	23.284669411810956	25.967651967210966	25.61975136445295	25.12792725652513
40	25.69889219924346	23.268601942112667	26.267065276659608	24.76544058198426
41	21.303926445174888	23.448420858310556	26.51952966755721	28.72812302895734
42	24.18296631699978	24.071006820697864	25.567560576354953	26.178466285947405
43	23.616673475399395	22.749314995906214	27.417712619060236	26.216298909634155
44	23.232820484770375	24.674163679655678	27.980074058500403	24.112941777073544
45	22.273729288205395	25.951698451198478	26.52420176867516	25.25037049192097
46	23.876146017973916	25.068671339907322	27.045881742283484	24.009300899835278
47	23.173849452367072	24.2916211564134	28.16536275161686	24.369166639602664
48	24.495484300315823	23.351104410634385	28.466599320038355	23.686811969011433
49	24.27663624673024	21.994428804301066	29.20359478297236	24.525340165996333
50	22.301192126484032	25.402954477211825	28.079042834620726	24.216810561683417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	653.0
1	1271.5
2	1890.0
3	10042.0
4	18194.0
5	12193.0
6	6192.0
7	6307.0
8	6422.0
9	6763.0
10	7104.0
11	7227.5
12	7351.0
13	7328.0
14	7305.0
15	6899.5
16	6494.0
17	6470.5
18	6447.0
19	6318.0
20	6189.0
21	6580.5
22	6972.0
23	7466.5
24	7961.0
25	8920.0
26	9879.0
27	11496.0
28	13113.0
29	15935.5
30	18758.0
31	21497.0
32	24236.0
33	26785.0
34	29334.0
35	33755.0
36	38176.0
37	44335.5
38	50495.0
39	61476.5
40	72458.0
41	84186.5
42	95915.0
43	104820.0
44	113725.0
45	124334.5
46	134944.0
47	141754.0
48	148564.0
49	145714.0
50	142864.0
51	131660.5
52	120457.0
53	114305.0
54	108153.0
55	103490.0
56	98827.0
57	95990.0
58	93153.0
59	88757.5
60	84362.0
61	79082.5
62	73803.0
63	65809.0
64	57815.0
65	50688.0
66	43561.0
67	37984.0
68	32407.0
69	27632.0
70	22857.0
71	19040.0
72	15223.0
73	12692.0
74	10161.0
75	8394.5
76	6628.0
77	4982.5
78	3337.0
79	2416.5
80	1496.0
81	1095.0
82	694.0
83	502.0
84	310.0
85	235.0
86	160.0
87	101.5
88	43.0
89	26.5
90	10.0
91	6.0
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1755099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.382093410742304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28100514762974	21.82531431891487
2	6.637465083559162	3.9004523819135155
3	2.7407954866960775	2.4159092702953524
4	1.7812991555886764	2.093531927279315
5	1.418009748585983	2.0832047445148283
6	1.1509377921787645	2.029017703184998
7	0.9531155997396715	1.9603172107950688
8	0.8391802401475936	1.9725497763572601
9	0.7889593656141195	2.0863149999978643
>10	9.239889214526533	54.43704371907041
>50	0.1440370033635489	2.713135483597922
>100	0.02291503999846108	1.30360718707918
>500	0.001793341778765753	0.3617022379046281
>1k	1.9926019764063922E-4	0.12845171737823796
>5k	3.9852039528127844E-4	0.6894473217165589
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6086	0.3467610658999863	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5690	0.32419823611089743	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2194	0.12500719332641633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05031055228223593	0.0
2	0.0	0.0	0.0	0.16608749705857048	0.0
3	0.0	0.0	0.0	0.26266324577701883	0.0
4	0.0	0.0	0.0	0.4231670122312189	0.0
5	0.0	0.0	0.0	0.70195470454943	0.0
6	0.0	0.0	0.0	1.206028833701119	0.0
7	0.0	0.0	0.0	1.4734211574389822	0.0
8	0.0	0.0	0.0	2.194178220145986	0.0
9	0.0	0.0	0.0	2.892942221492919	0.0
10	0.0	0.0	0.0	3.855509005474905	0.0
11	0.0	0.0	0.0	4.488293822741623	0.0
12	0.0	0.0	0.0	5.032308718767431	0.0
13	0.0	0.0	0.0	5.314913859560059	0.0
14	0.0	0.0	0.0	5.457868758400523	0.0
15	0.0	0.0	0.0	5.591479455005103	0.0
16	0.0	0.0	0.0	5.82593916354576	0.0
17	0.0	0.0	0.0	6.07390238385413	0.0
18	0.0	0.0	0.0	6.439579761597494	0.0
19	0.0	0.0	0.0	6.610282382931105	0.0
20	0.0	0.0	0.0	6.814601341576743	0.0
21	0.0	0.0	0.0	7.029176131944694	0.0
22	0.0	0.0	0.0	7.244320690741662	0.0
23	0.0	0.0	0.0	7.479976912983256	0.0
24	0.0	0.0	0.0	7.675350507293321	0.0
25	0.0	0.0	0.0	7.844913591768897	0.0
26	0.0	0.0	0.0	8.00649991823823	0.0
27	0.0	0.0	0.0	8.183526969133935	0.0
28	0.0	0.0	0.0	8.347506323005142	0.0
29	0.0	0.0	0.0	8.530857803462938	0.0
30	0.0	0.0	0.0	8.764975650946186	0.0
31	0.0	0.0	0.0	8.956816680996342	0.0
32	0.0	0.0	0.0	9.169739142920143	0.0
33	0.0	0.0	0.0	9.370582514148774	0.0
34	0.0	0.0	0.0	9.580884041299095	0.0
35	0.0	0.0	0.0	9.80280884440137	0.0
36	0.0	0.0	0.0	9.99311149969318	0.0
37	0.0	0.0	0.0	10.195721153051766	0.0
38	0.0	0.0	0.0	10.443741350203037	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	25	4.443802E-5	44.0	1
CGTAAGA	115	0.0	44.0	2
TGATTCG	160	0.0	42.625004	15
ATTGCGA	150	0.0	42.533333	2
AGCGATA	385	0.0	40.571426	8
CGTTAGG	120	0.0	40.333332	2
CGTTTTT	6250	0.0	40.268803	1
TATAGCG	55	7.8216544E-11	40.0	1
CGCATCG	45	2.3521352E-8	39.11111	21
GAGCGAT	1925	0.0	39.085712	7
TATGCGA	130	0.0	38.923077	2
CTATCTC	860	0.0	38.883724	6
GTTTTTA	3475	0.0	38.04892	2
CCGTTTA	35	7.2924013E-6	37.714287	13
TCGTAAG	65	1.0913936E-11	37.230766	1
TATCACG	60	1.9826984E-10	36.666664	1
ATTACCG	30	1.3013753E-4	36.666664	1
ATTCGGC	190	0.0	35.894737	17
GCGTTAG	190	0.0	35.894737	1
AACCGTT	405	0.0	35.851852	44
>>END_MODULE