FastQCFastQC Report
Thu 26 May 2016
SRR1546711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546711_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2255356
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137020.6075315825971598No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123560.5478514256729314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT700.044.06
GTTAACG351.4469151E-744.01
CGTTTTT153950.042.2279971
GTTTTTA83700.040.5567472
GAGCGAT23500.040.536177
CTATCTC13100.038.961836
ACGTAAG1100.038.01
ACGGGAT2500.037.845
ATGTCTC23250.037.565598
AGCGATA5150.037.1650478
TATACTA13750.036.9644
GTTGATC10100.036.81188216
TATTTTT96300.036.4839067
GATGTCT23950.036.2839247
TTATTTT97150.036.2552766
TATACGA850.036.235292
CGTAAGA1950.036.1025662
ATCGCAT1100.036.010
TACCGGT553.428795E-936.040
GCCAGTA10100.035.94059425
Produced by FastQC (version 0.11.5)