##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546711_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2255356
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43831262115604	31.0	31.0	33.0	30.0	34.0
2	31.81615274927772	31.0	31.0	34.0	30.0	34.0
3	31.91854722713399	31.0	31.0	34.0	30.0	34.0
4	35.63849254840478	37.0	35.0	37.0	33.0	37.0
5	35.587542720528376	37.0	35.0	37.0	33.0	37.0
6	35.61093104591914	37.0	35.0	37.0	33.0	37.0
7	35.97467849864944	37.0	35.0	37.0	35.0	37.0
8	18.410413699655397	30.0	0.0	37.0	0.0	37.0
9	27.856349064183217	28.0	17.0	39.0	17.0	39.0
10	33.46215675041989	32.0	32.0	39.0	27.0	39.0
11	35.6807178112901	37.0	34.0	39.0	32.0	39.0
12	36.46682829673009	37.0	35.0	39.0	32.0	39.0
13	36.72306278920046	39.0	35.0	39.0	33.0	39.0
14	37.8405577656033	40.0	37.0	41.0	33.0	41.0
15	38.03903951305249	40.0	37.0	41.0	33.0	41.0
16	38.09281239857477	40.0	37.0	41.0	33.0	41.0
17	38.03841034408759	40.0	37.0	41.0	33.0	41.0
18	37.99241228435777	40.0	37.0	41.0	33.0	41.0
19	37.981759864074675	40.0	37.0	41.0	33.0	41.0
20	37.87399638904014	40.0	36.0	41.0	33.0	41.0
21	37.730438564909484	40.0	36.0	41.0	33.0	41.0
22	37.72280251986826	40.0	36.0	41.0	33.0	41.0
23	37.5454970301806	39.0	36.0	41.0	33.0	41.0
24	37.34244793283189	39.0	35.0	41.0	32.0	41.0
25	37.180973203343505	39.0	35.0	41.0	32.0	41.0
26	37.146321467653	39.0	35.0	41.0	32.0	41.0
27	37.015383380716834	39.0	35.0	41.0	32.0	41.0
28	36.97520391459264	39.0	35.0	41.0	32.0	41.0
29	36.84611475970978	39.0	35.0	40.0	31.0	41.0
30	36.68762625501251	38.0	35.0	40.0	31.0	41.0
31	36.66274237858679	39.0	35.0	40.0	31.0	41.0
32	36.40705547150871	39.0	35.0	41.0	30.0	41.0
33	36.22301268624554	39.0	35.0	41.0	30.0	41.0
34	35.97893325931693	39.0	35.0	41.0	29.0	41.0
35	35.74562109041766	39.0	35.0	41.0	27.0	41.0
36	35.57576098850913	38.0	35.0	40.0	26.0	41.0
37	35.53474130026479	38.0	35.0	40.0	26.0	41.0
38	35.425090318335556	38.0	35.0	40.0	26.0	41.0
39	35.358429888673896	38.0	35.0	40.0	25.0	41.0
40	35.265944267778565	38.0	35.0	40.0	25.0	41.0
41	35.224463454993355	38.0	35.0	40.0	25.0	41.0
42	35.048393247008455	38.0	34.0	40.0	24.0	41.0
43	35.030649263353546	38.0	34.0	40.0	24.0	41.0
44	34.7585503131213	38.0	34.0	40.0	23.0	41.0
45	34.63026058857227	38.0	34.0	40.0	23.0	41.0
46	34.626072336252015	37.0	34.0	40.0	23.0	41.0
47	34.490235244458084	37.0	34.0	40.0	23.0	41.0
48	34.450652136514144	37.0	34.0	40.0	23.0	41.0
49	34.40455076715162	37.0	33.0	40.0	23.0	41.0
50	33.77273432664289	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	5.0
12	3.0
13	9.0
14	23.0
15	43.0
16	123.0
17	314.0
18	777.0
19	1645.0
20	3187.0
21	5318.0
22	8389.0
23	12665.0
24	18754.0
25	27784.0
26	37747.0
27	43581.0
28	45332.0
29	49126.0
30	57162.0
31	70081.0
32	89003.0
33	116853.0
34	169221.0
35	235485.0
36	252251.0
37	356648.0
38	356165.0
39	297657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.33866405126286	23.70100330058758	26.827427687690992	17.13290496045857
2	34.09217879572006	24.846897784651294	27.252061315375485	13.808862104253164
3	28.48725434033474	24.787794033403152	33.29549747356958	13.429454152692525
4	24.924801228719545	27.297774719379113	32.74791208128561	15.029511970615724
5	23.11147331064364	29.976154540569205	32.106860291679006	14.805511857108147
6	20.9229052974342	38.43366634801779	29.54438235028084	11.099046004267175
7	82.49429358380672	4.073325896222149	10.997731621970102	2.434648898001025
8	42.01154939619288	50.54798444236741	5.416572815998894	2.0238933454408086
9	75.85742561263056	5.2933106791123	14.271183795374212	4.578079912882933
10	40.07908285875932	24.494492222070484	20.466037290786907	14.960387628383279
11	32.15851510803616	24.122134155317386	27.38192994808802	16.337420788558436
12	27.901493156734457	21.119770005267462	31.102185198256947	19.876551639741134
13	23.525509941667746	25.051300105171865	32.9313421029762	18.491847850184186
14	20.12990410383106	26.537140921433245	33.78193952529002	19.551015449445675
15	19.55465123909485	21.84976562458432	39.51349587382214	19.082087262498693
16	22.273867185490897	20.857328067054603	36.665830139454705	20.202974607999803
17	21.709033961822435	22.657265637885992	32.33502826161369	23.298672138677883
18	23.836414295570187	21.71697062459319	33.51564010293719	20.93097497689943
19	26.264146325458153	25.94849770945252	28.072596964736384	19.714759000352938
20	28.104875682597335	24.061655898226267	27.5170305707835	20.316437848392894
21	25.06721776961154	26.66262000322787	28.73666064248837	19.53350158467222
22	23.736474419116096	23.175055290606007	29.008103377027837	24.08036691325006
23	22.791302127025624	26.195288016614672	28.60116096970944	22.412248886650268
24	22.542915619529687	23.566700778058987	33.14886873735233	20.741514865058996
25	22.99849779813032	24.739819345593332	29.9896779045082	22.272004951768146
26	21.041556188912082	25.84687295486832	30.07746892286628	23.034101933353316
27	21.851361824918104	24.284946589363276	30.254469804323573	23.609221781395043
28	21.4866743875468	25.617108784599857	31.28228980258549	21.613927025267852
29	22.791036093636656	24.10790136900782	30.144731031375976	22.956331505979545
30	24.117788943297644	24.09974301174626	29.821101413701427	21.961366631254666
31	25.78147308008137	24.514267370650135	27.174645599187002	22.529613950081494
32	27.1935783087016	23.543289839830166	26.403281787886257	22.85985006358198
33	26.235725091737176	25.392487926518033	25.33076818027841	23.041018801466375
34	23.340749753032338	26.009241999932602	28.225699180085094	22.424309066949963
35	23.864835529291163	26.31079084632315	27.796542984788207	22.02783063959747
36	25.78768052582386	27.69611538045435	25.490255196962252	21.025948896759537
37	23.660388869872428	27.560305335388293	25.94854204835068	22.830763746388598
38	23.395774325649697	27.45229577946896	26.981594036595553	22.170335858285785
39	22.805712268927834	25.788522964888916	25.720817467397612	25.684947298785648
40	24.360544410727176	23.518593073554687	28.508492672553693	23.612369843164448
41	20.776187883420622	23.655334235482115	28.274161595774682	27.294316285322584
42	23.38703956271205	25.18835163938642	26.88240792141019	24.54220087649134
43	22.434063624545306	23.932319332291666	28.810307552333203	24.82330949082983
44	23.60966517037665	25.551176843034977	27.809401265254795	23.029756721333573
45	21.65112736082463	26.09202272279853	27.871032333698093	24.385817582678744
46	23.394665853195683	25.969470008282507	27.411237959772205	23.22462617874961
47	22.51130198514115	24.949453656096864	28.543919452184046	23.99532490657794
48	23.156920681258303	23.810919429127818	29.11407334363178	23.9180865459821
49	23.654092746333617	23.10114234737221	28.550038220130215	24.69472668616396
50	21.85974187667047	24.80415508682443	29.543938961299236	23.792164075205864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	901.0
1	1642.5
2	2384.0
3	21540.0
4	40696.0
5	27907.0
6	15118.0
7	15223.0
8	15328.0
9	15727.0
10	16126.0
11	16096.0
12	16066.0
13	15759.0
14	15452.0
15	14828.5
16	14205.0
17	13515.0
18	12825.0
19	12351.5
20	11878.0
21	11944.5
22	12011.0
23	12676.0
24	13341.0
25	14589.0
26	15837.0
27	18320.0
28	20803.0
29	23628.5
30	26454.0
31	30205.5
32	33957.0
33	39186.0
34	44415.0
35	50721.5
36	57028.0
37	62879.0
38	68730.0
39	78506.0
40	88282.0
41	99527.5
42	110773.0
43	118041.5
44	125310.0
45	136607.5
46	147905.0
47	163727.0
48	179549.0
49	191582.0
50	203615.0
51	193080.0
52	182545.0
53	164177.0
54	145809.0
55	133340.5
56	120872.0
57	114952.5
58	109033.0
59	102715.0
60	96397.0
61	89847.5
62	83298.0
63	74185.0
64	65072.0
65	56095.5
66	47119.0
67	40265.5
68	33412.0
69	28410.0
70	23408.0
71	19844.0
72	16280.0
73	13343.0
74	10406.0
75	8313.0
76	6220.0
77	4858.5
78	3497.0
79	2580.5
80	1664.0
81	1196.5
82	729.0
83	556.5
84	384.0
85	271.0
86	158.0
87	99.5
88	41.0
89	30.0
90	19.0
91	10.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2255356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.26146298734361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.71337328488312	28.21191056301603
2	6.866634346473154	5.117216830974637
3	3.106768674712721	3.472882379491398
4	2.0524679306377065	3.0591183132066653
5	1.5931589010120861	2.968171571150944
6	1.2660757454225742	2.830550071634204
7	1.11943394339369	2.9198222513978065
8	0.9102345163041546	2.7133335791255897
9	0.8354429909890465	2.8016845278096607
>10	6.4490398978884516	40.6820944728548
>50	0.06370159366081109	1.5562020670458638
>100	0.020466347157992212	1.4465958841708564
>500	0.002366568125955267	0.5690837794276856
>1k	5.56839559048298E-4	0.29966247322520645
>5k	0.0	0.0
>10k+	2.78419779524149E-4	1.3516712354686566
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13702	0.6075315825971598	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12356	0.5478514256729314	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04832939899510321	0.0
2	0.0	0.0	0.0	0.1358100450660561	0.0
3	0.0	0.0	0.0	0.2327348764452264	0.0
4	0.0	0.0	0.0	0.38725593653507473	0.0
5	0.0	0.0	0.0	0.6453526627281901	0.0
6	0.0	0.0	0.0	1.274743322118548	0.0
7	0.0	0.0	0.0	1.6613341751812132	0.0
8	0.0	0.0	0.0	2.646721847903391	0.0
9	0.0	0.0	0.0	3.789911659179305	0.0
10	0.0	0.0	0.0	5.205785694143186	0.0
11	0.0	0.0	0.0	5.9489499662137595	0.0
12	0.0	0.0	0.0	6.587829149810496	0.0
13	0.0	0.0	0.0	6.963290939434839	0.0
14	0.0	0.0	0.0	7.163968792509919	0.0
15	0.0	0.0	0.0	7.314543690663469	0.0
16	0.0	0.0	0.0	7.544884266608021	0.0
17	0.0	0.0	0.0	7.772741864255576	0.0
18	0.0	0.0	0.0	8.132108633847606	0.0
19	0.0	0.0	0.0	8.30432091430355	0.0
20	0.0	0.0	0.0	8.508944929314929	0.0
21	0.0	0.0	0.0	8.710021832473455	0.0
22	0.0	0.0	0.0	8.919523126282503	0.0
23	0.0	0.0	0.0	9.158022059488612	0.0
24	0.0	0.0	0.0	9.358877268156336	0.0
25	0.0	0.0	0.0	9.529315992685856	0.0
26	0.0	0.0	0.0	9.693015204694957	0.0
27	0.0	0.0	0.0	9.872410386652927	0.0
28	0.0	0.0	0.0	10.042583077793484	0.0
29	0.0	0.0	0.0	10.229515872438764	0.0
30	0.0	0.0	0.0	10.480562713824336	0.0
31	0.0	0.0	0.0	10.67804816623185	0.0
32	0.0	0.0	0.0	10.902669024313678	0.0
33	0.0	0.0	0.0	11.111283540159514	0.0
34	0.0	0.0	0.0	11.315419827291123	0.0
35	0.0	0.0	0.0	11.54332176383684	0.0
36	0.0	0.0	0.0	11.738235560151036	0.0
37	0.0	0.0	0.0	11.946451025913426	0.0
38	0.0	0.0	0.0	12.195458277983608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAT	70	0.0	44.0	6
GTTAACG	35	1.4469151E-7	44.0	1
CGTTTTT	15395	0.0	42.227997	1
GTTTTTA	8370	0.0	40.556747	2
GAGCGAT	2350	0.0	40.53617	7
CTATCTC	1310	0.0	38.96183	6
ACGTAAG	110	0.0	38.0	1
ACGGGAT	250	0.0	37.84	5
ATGTCTC	2325	0.0	37.56559	8
AGCGATA	515	0.0	37.165047	8
TATACTA	1375	0.0	36.96	44
GTTGATC	1010	0.0	36.811882	16
TATTTTT	9630	0.0	36.483906	7
GATGTCT	2395	0.0	36.283924	7
TTATTTT	9715	0.0	36.255276	6
TATACGA	85	0.0	36.23529	2
CGTAAGA	195	0.0	36.102566	2
ATCGCAT	110	0.0	36.0	10
TACCGGT	55	3.428795E-9	36.0	40
GCCAGTA	1010	0.0	35.940594	25
>>END_MODULE