FastQCFastQC Report
Thu 26 May 2016
SRR1546710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546710_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1537669
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.27366097645201926No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38610.25109435125504903No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22300.14502470947908816No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGT207.857311E-444.00000416
GTATCGA254.443544E-544.02
TATCGTT254.443544E-544.012
AGCGATA3050.042.5573778
GAGCGAT14850.040.740747
TACGTTG650.040.6153831
CGTTTTT47100.040.076431
GCGATAT1500.038.133339
TACGTAG750.038.133331
CGCAATC2500.037.8420
TAATCGT2100.037.71428721
CGTAAGA1350.037.481482
CGAGTAC651.0913936E-1137.2307666
TTAATCG2150.036.83720820
CTATCTC9400.036.744686
ACGATAA301.3013E-436.6666648
ACGTAAG601.9826984E-1036.6666641
GTTTTTA30500.036.570492
CACAACG3050.036.06557512
TTTACGG1050.035.619052
Produced by FastQC (version 0.11.5)