##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546710_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1537669
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.468717259696334	31.0	31.0	33.0	30.0	34.0
2	31.848840680276446	31.0	31.0	34.0	30.0	34.0
3	31.972379621361945	31.0	31.0	34.0	30.0	34.0
4	35.672417796027624	37.0	35.0	37.0	33.0	37.0
5	35.60260173028136	37.0	35.0	37.0	33.0	37.0
6	35.637204756030066	37.0	35.0	37.0	33.0	37.0
7	35.98823413881661	37.0	35.0	37.0	35.0	37.0
8	18.67938743643788	32.0	0.0	37.0	0.0	37.0
9	28.02370926382726	31.0	17.0	39.0	17.0	39.0
10	33.59995876875973	32.0	32.0	39.0	27.0	39.0
11	35.72909384269306	37.0	35.0	39.0	32.0	39.0
12	36.417657506264355	37.0	35.0	39.0	32.0	39.0
13	36.6125336467081	39.0	35.0	39.0	33.0	39.0
14	37.736770397270156	39.0	36.0	41.0	33.0	41.0
15	37.9506623337012	40.0	36.0	41.0	33.0	41.0
16	38.02953561527221	40.0	36.0	41.0	33.0	41.0
17	37.98024412275984	40.0	36.0	41.0	33.0	41.0
18	37.95453117673569	40.0	36.0	41.0	33.0	41.0
19	37.96822463091862	40.0	36.0	41.0	33.0	41.0
20	37.8943790893879	40.0	36.0	41.0	33.0	41.0
21	37.750929491327454	40.0	36.0	41.0	33.0	41.0
22	37.73723733781458	40.0	36.0	41.0	33.0	41.0
23	37.60317727677413	39.0	36.0	41.0	33.0	41.0
24	37.49148288740945	39.0	35.0	41.0	33.0	41.0
25	37.33183864667884	39.0	35.0	41.0	32.0	41.0
26	37.29756794212538	39.0	35.0	41.0	32.0	41.0
27	37.15684194712907	39.0	35.0	41.0	32.0	41.0
28	37.17095941974508	39.0	35.0	41.0	32.0	41.0
29	37.06890104437301	39.0	35.0	41.0	32.0	41.0
30	36.958428634511066	39.0	35.0	40.0	31.0	41.0
31	36.964716073485256	39.0	35.0	41.0	31.0	41.0
32	36.81379867838917	39.0	35.0	41.0	31.0	41.0
33	36.696753332479226	39.0	35.0	41.0	31.0	41.0
34	36.61459846039687	39.0	35.0	41.0	31.0	41.0
35	36.42275027980664	39.0	35.0	41.0	30.0	41.0
36	36.31262905085555	39.0	35.0	41.0	30.0	41.0
37	36.221445577689344	39.0	35.0	40.0	30.0	41.0
38	36.14563472372793	39.0	35.0	40.0	30.0	41.0
39	36.0483309476877	38.0	35.0	40.0	30.0	41.0
40	35.95770416129869	38.0	35.0	40.0	29.0	41.0
41	35.9254202302316	38.0	35.0	40.0	29.0	41.0
42	35.759099650184794	38.0	35.0	40.0	29.0	41.0
43	35.73307064134089	38.0	35.0	40.0	29.0	41.0
44	35.461590238211215	38.0	35.0	40.0	28.0	41.0
45	35.3403658394622	38.0	34.0	40.0	27.0	41.0
46	35.332070165945986	38.0	34.0	40.0	28.0	41.0
47	35.17992623900202	37.0	34.0	40.0	27.0	41.0
48	35.12846002618249	37.0	34.0	40.0	27.0	41.0
49	35.09251991163248	37.0	34.0	40.0	27.0	41.0
50	34.43609775575888	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	8.0
13	7.0
14	16.0
15	30.0
16	68.0
17	183.0
18	425.0
19	955.0
20	1722.0
21	2898.0
22	4521.0
23	6664.0
24	9713.0
25	13264.0
26	17364.0
27	20728.0
28	24004.0
29	29320.0
30	36067.0
31	46659.0
32	60494.0
33	80136.0
34	120492.0
35	177535.0
36	172301.0
37	248893.0
38	256104.0
39	207094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.57978862811177	24.18394335842109	28.425883593933417	13.810384419533722
2	31.23058343505657	25.813292717743547	29.158616061063857	13.797507786136029
3	29.490091820801485	25.841062023101202	30.556055952223787	14.112790203873526
4	25.73017990217661	28.701105374433638	30.19609551860641	15.372619204783344
5	23.87958656902103	31.441422048568317	29.576521344970864	15.102470037439787
6	21.482386651483512	40.34047639641562	26.56579536948459	11.611341582616284
7	86.02989329953326	4.348725245810379	7.184901301905677	2.4364801527506894
8	44.532015667871306	49.97727079104801	3.3819372049511305	2.1087763361295573
9	79.77542631086403	5.410527233104133	10.210650016355926	4.603396439675899
10	45.90851477138448	23.94006772588899	15.216798933970836	14.934618568755695
11	36.53126908326825	23.917240966683988	23.701980075035657	15.849509875012114
12	31.43947104350806	21.680998966617654	26.762846880570525	20.116683109303757
13	23.397233084623544	28.792347377751646	29.141122049023554	18.669297488601252
14	19.815317860996092	30.65991445493146	29.911834081326994	19.612933602745457
15	19.051304279399535	22.545489308817437	39.14054325085568	19.262663160927353
16	21.705191429364838	20.62992750715531	36.8514940471584	20.813387016321457
17	21.665520993139616	22.5300113353394	28.74910009891596	27.05536757260503
18	24.041650056026363	22.645510834906602	31.413002408190575	21.899836700876456
19	28.443117471965685	25.32092407403674	25.262328888727026	20.97362956527055
20	29.61261493858561	24.075987745086884	24.321033980655134	21.990363335672374
21	25.877870985238044	27.523738854070672	26.148930621609722	20.44945953908156
22	24.989968582315182	24.442971796921185	25.600958333685597	24.96610128707804
23	23.578676555227425	28.100390916380576	24.467814594688452	23.853117933703547
24	23.178265283360723	23.917175933181976	32.13097227036508	20.77358651309222
25	22.97835229818641	24.357257641273904	29.018078663223363	23.646311397316328
26	20.932203224491097	28.1286804897543	27.226340649385534	23.712775636369077
27	22.829750746096853	26.0013696055523	27.90821691794528	23.260662730405567
28	21.698883179670005	26.451466472953545	30.97513183916695	20.874518508209505
29	22.835213560265572	24.1406960795854	29.884910211495452	23.13918014865358
30	25.524804102833578	25.121401289874477	27.958487814997895	21.39530679229405
31	28.364817135547376	23.87652999442663	24.937291445688246	22.821361424337745
32	31.098890593489237	23.738203735654423	24.493567861483843	20.669337809372497
33	27.673966243710446	24.442126361395072	26.041950510805638	21.84195688408884
34	25.64290494248112	25.160486424581624	27.896315787077715	21.300292845859545
35	24.52940132109056	24.854698898137375	29.556035791838163	21.0598639889339
36	29.981810129488206	25.198986257770688	24.866404928498916	19.952798684242186
37	25.09200614696661	28.739540174120698	26.39748866628644	19.770965012626256
38	26.31931839687215	28.42003057875264	24.275835696759184	20.984815327616023
39	24.61433507471374	28.481552271652745	24.59293905255292	22.311173601080597
40	26.71719336216052	24.233368819947597	26.015091674476103	23.03434614341578
41	21.553663369684894	24.422616310792506	26.958337587608256	27.065382731914344
42	25.579952512536835	25.400329979989188	24.248586659417597	24.771130848056377
43	23.75238103909229	24.21372870234101	27.42332712696946	24.610563131597242
44	23.76408706945383	26.0515104356009	27.236615942702887	22.947786552242388
45	21.931963250868687	27.278952752510456	26.82137703237823	23.96770696424263
46	24.938592115728415	26.103667304211765	26.281208764695137	22.676531815364683
47	24.008157802491954	24.46885513072059	27.625256150706036	23.897730916081418
48	24.63423532632836	23.11336184835618	28.586776477902593	23.665626347412868
49	24.404016729218057	22.77655334145385	28.154498790051697	24.664931139276398
50	22.302524145313456	26.085197789641335	28.361760560952977	23.250517504092233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	683.0
1	1175.5
2	1668.0
3	7432.5
4	13197.0
5	9056.5
6	4916.0
7	5219.0
8	5522.0
9	5686.5
10	5851.0
11	6014.5
12	6178.0
13	6232.0
14	6286.0
15	6194.5
16	6103.0
17	5882.5
18	5662.0
19	5569.5
20	5477.0
21	5992.0
22	6507.0
23	7060.0
24	7613.0
25	8207.5
26	8802.0
27	10229.0
28	11656.0
29	14015.5
30	16375.0
31	19603.5
32	22832.0
33	25576.0
34	28320.0
35	31955.0
36	35590.0
37	40675.0
38	45760.0
39	57109.0
40	68458.0
41	79806.5
42	91155.0
43	98997.0
44	106839.0
45	116932.0
46	127025.0
47	132715.5
48	138406.0
49	135061.5
50	131717.0
51	119800.0
52	107883.0
53	98940.5
54	89998.0
55	84655.0
56	79312.0
57	77327.0
58	75342.0
59	71739.5
60	68137.0
61	63618.5
62	59100.0
63	52325.0
64	45550.0
65	39265.0
66	32980.0
67	28608.0
68	24236.0
69	20987.5
70	17739.0
71	14956.0
72	12173.0
73	9838.5
74	7504.0
75	5852.5
76	4201.0
77	3399.5
78	2598.0
79	2040.0
80	1482.0
81	984.5
82	487.0
83	332.5
84	178.0
85	144.5
86	111.0
87	75.5
88	40.0
89	26.0
90	12.0
91	9.0
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1537669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.67334067737733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01845570601031	20.2066460048841
2	6.4652512622039975	3.5783020148763
3	2.963298263094753	2.4601308708990484
4	1.8958166315727707	2.098543180294049
5	1.4196974241680076	1.964388523889817
6	1.1958681997370253	1.9856200857938773
7	1.0416679183423263	2.0178501823887163
8	0.9068586715298168	2.0076647170782755
9	0.8516469219464519	2.1211123867077544
>10	10.049865020244395	56.25704281623363
>50	0.16277382916449376	2.8388706189515154
>100	0.02502308497975008	1.229138115450149
>500	0.003068866942279028	0.5618821608696577
>1k	7.082000636028526E-4	0.6728083216831304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4208	0.27366097645201926	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3861	0.25109435125504903	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2230	0.14502470947908816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0532624381450104	0.0
2	0.0	0.0	0.0	0.15458463427434643	0.0
3	0.0	0.0	0.0	0.25460616036351125	0.0
4	0.0	0.0	0.0	0.4352041954412816	0.0
5	0.0	0.0	0.0	0.6645773570254717	0.0
6	0.0	0.0	0.0	1.2636659775283237	0.0
7	0.0	0.0	0.0	1.5814847018441551	0.0
8	0.0	0.0	0.0	2.572009970936528	0.0
9	0.0	0.0	0.0	3.556031889828045	0.0
10	0.0	0.0	0.0	4.735154314745241	0.0
11	0.0	0.0	0.0	5.402983346871141	0.0
12	0.0	0.0	0.0	5.971311120923944	0.0
13	6.503350200855971E-5	0.0	0.0	6.2926416543482375	0.0
14	1.3006700401711941E-4	0.0	0.0	6.449632528196901	0.0
15	1.3006700401711941E-4	0.0	0.0	6.573196182013164	0.0
16	1.3006700401711941E-4	0.0	0.0	6.772979100183459	0.0
17	1.3006700401711941E-4	0.0	0.0	6.9738675878879	0.0
18	1.3006700401711941E-4	0.0	0.0	7.271395859577061	0.0
19	1.3006700401711941E-4	0.0	0.0	7.416095401546107	0.0
20	1.3006700401711941E-4	0.0	0.0	7.586353109804516	0.0
21	1.9510050602567912E-4	0.0	0.0	7.762528866745704	0.0
22	1.9510050602567912E-4	0.0	0.0	7.94377723684356	0.0
23	1.9510050602567912E-4	0.0	0.0	8.149218069688601	0.0
24	1.9510050602567912E-4	0.0	0.0	8.311541690701965	0.0
25	1.9510050602567912E-4	0.0	0.0	8.448372178927976	0.0
26	1.9510050602567912E-4	0.0	0.0	8.585072600149967	0.0
27	1.9510050602567912E-4	0.0	0.0	8.742713809018715	0.0
28	1.9510050602567912E-4	0.0	0.0	8.878048526698528	0.0
29	1.9510050602567912E-4	0.0	0.0	9.025024241237874	0.0
30	1.9510050602567912E-4	0.0	0.0	9.225132326918212	0.0
31	1.9510050602567912E-4	0.0	0.0	9.381537899248798	0.0
32	1.9510050602567912E-4	0.0	0.0	9.552901177041353	0.0
33	1.9510050602567912E-4	0.0	0.0	9.709111648865914	0.0
34	1.9510050602567912E-4	0.0	0.0	9.874947078987741	0.0
35	1.9510050602567912E-4	0.0	0.0	10.034994527430806	0.0
36	1.9510050602567912E-4	0.0	0.0	10.191139965753358	0.0
37	1.9510050602567912E-4	0.0	0.0	10.358796333931425	0.0
38	1.9510050602567912E-4	0.0	0.0	10.550124896840607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGGT	20	7.857311E-4	44.000004	16
GTATCGA	25	4.443544E-5	44.0	2
TATCGTT	25	4.443544E-5	44.0	12
AGCGATA	305	0.0	42.557377	8
GAGCGAT	1485	0.0	40.74074	7
TACGTTG	65	0.0	40.615383	1
CGTTTTT	4710	0.0	40.07643	1
GCGATAT	150	0.0	38.13333	9
TACGTAG	75	0.0	38.13333	1
CGCAATC	250	0.0	37.84	20
TAATCGT	210	0.0	37.714287	21
CGTAAGA	135	0.0	37.48148	2
CGAGTAC	65	1.0913936E-11	37.230766	6
TTAATCG	215	0.0	36.837208	20
CTATCTC	940	0.0	36.74468	6
ACGATAA	30	1.3013E-4	36.666664	8
ACGTAAG	60	1.9826984E-10	36.666664	1
GTTTTTA	3050	0.0	36.57049	2
CACAACG	305	0.0	36.065575	12
TTTACGG	105	0.0	35.61905	2
>>END_MODULE