FastQCFastQC Report
Thu 26 May 2016
SRR1546709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546709_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3154412
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111220.3525855214854623No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33310.1055981273213518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA1600.041.252
CGTTTTT126450.040.5725561
CGTAAGA1850.040.432432
GAGCGAT29950.040.327217
CTATCTC15850.037.8927466
TGCGTAG2550.037.098041
GTTTTTA74900.036.862482
AGCGATA6950.036.7194258
GTAACGA900.036.6666682
TAATGCG1450.036.4137951
TGATTCG5150.036.3106815
CCGCAAT1700.036.23529433
TAAGCGA1600.035.752
ATTCGGC5350.035.36448717
CGCATTA250.002353365535.230
CGTTAGT250.002353365535.241
CGTAATT250.002353365535.213
CTAAGCG950.034.7368431
AGACCGA10050.034.5870678
GACCGAT21350.034.519919
Produced by FastQC (version 0.11.5)