##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546709_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3154412
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.491116886443496	31.0	31.0	33.0	30.0	34.0
2	31.871035869759563	31.0	31.0	34.0	30.0	34.0
3	31.982488336970565	33.0	31.0	34.0	30.0	34.0
4	35.678242093930656	37.0	35.0	37.0	33.0	37.0
5	35.61396545536854	37.0	35.0	37.0	33.0	37.0
6	35.64772325238428	37.0	35.0	37.0	33.0	37.0
7	36.003389221192414	37.0	35.0	37.0	35.0	37.0
8	18.734155208641102	32.0	0.0	37.0	0.0	37.0
9	28.057891613397363	32.0	17.0	39.0	17.0	39.0
10	33.6067910596333	32.0	32.0	39.0	27.0	39.0
11	35.74600717978501	37.0	35.0	39.0	32.0	39.0
12	36.44401270347691	37.0	35.0	39.0	32.0	39.0
13	36.66042514421071	39.0	35.0	39.0	33.0	39.0
14	37.780490944112564	39.0	36.0	41.0	33.0	41.0
15	37.979921773059445	40.0	36.0	41.0	33.0	41.0
16	38.0399941415389	40.0	36.0	41.0	33.0	41.0
17	37.98367809911958	40.0	36.0	41.0	33.0	41.0
18	37.95763552763558	40.0	36.0	41.0	33.0	41.0
19	37.976194612498304	40.0	36.0	41.0	33.0	41.0
20	37.90670495800802	40.0	36.0	41.0	33.0	41.0
21	37.770084567266416	40.0	36.0	41.0	33.0	41.0
22	37.75769810665189	40.0	36.0	41.0	33.0	41.0
23	37.62001000503422	39.0	36.0	41.0	33.0	41.0
24	37.493368653175295	39.0	35.0	41.0	33.0	41.0
25	37.3425193031221	39.0	35.0	41.0	32.0	41.0
26	37.31535544500845	39.0	35.0	41.0	32.0	41.0
27	37.17192808041562	39.0	35.0	41.0	32.0	41.0
28	37.16651312510858	39.0	35.0	41.0	32.0	41.0
29	37.0640049555987	39.0	35.0	41.0	32.0	41.0
30	36.944523416725524	39.0	35.0	40.0	31.0	41.0
31	36.94195843789588	39.0	35.0	41.0	31.0	41.0
32	36.76251390116447	39.0	35.0	41.0	31.0	41.0
33	36.63776513657696	39.0	35.0	41.0	31.0	41.0
34	36.49162284444771	39.0	35.0	41.0	30.0	41.0
35	36.301913320137004	39.0	35.0	41.0	30.0	41.0
36	36.16269022562684	39.0	35.0	41.0	30.0	41.0
37	36.11154091475685	39.0	35.0	41.0	30.0	41.0
38	35.97182517692679	39.0	35.0	40.0	29.0	41.0
39	35.892188781934635	39.0	35.0	40.0	29.0	41.0
40	35.78869215562203	38.0	35.0	40.0	28.0	41.0
41	35.75381338899294	38.0	35.0	40.0	28.0	41.0
42	35.58123954638773	38.0	35.0	40.0	28.0	41.0
43	35.56813028862432	38.0	35.0	40.0	28.0	41.0
44	35.30493258331505	38.0	34.0	40.0	27.0	41.0
45	35.17621984699526	38.0	34.0	40.0	27.0	41.0
46	35.14293503828923	37.0	34.0	40.0	27.0	41.0
47	34.963541858197345	37.0	34.0	40.0	26.0	41.0
48	34.88920343949998	37.0	34.0	40.0	26.0	41.0
49	34.81136896511933	37.0	34.0	40.0	26.0	41.0
50	34.16484308327511	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	7.0
12	5.0
13	20.0
14	26.0
15	63.0
16	146.0
17	371.0
18	881.0
19	1933.0
20	3699.0
21	6238.0
22	9625.0
23	14479.0
24	21096.0
25	29902.0
26	39935.0
27	47186.0
28	53631.0
29	61915.0
30	75123.0
31	96092.0
32	123326.0
33	163118.0
34	241880.0
35	351373.0
36	350214.0
37	511997.0
38	520737.0
39	429393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.03538409060072	25.194489495982136	28.583266865583823	14.186859547833318
2	32.375891291308804	26.29374349324058	27.89321749980662	13.437147715643993
3	29.333073802661165	25.28734356831004	31.00397158012333	14.375611048905471
4	25.77630949920302	27.92574337150632	30.648279298962848	15.649667830327807
5	23.70593949046605	30.816329636077977	30.09251803505693	15.385212838399042
6	21.89523752762797	39.51665159782552	27.1500362032607	11.438074671285806
7	84.9659778113956	4.3755539859726635	8.22682642597099	2.4316417766607534
8	44.33396778860846	49.71741167609051	4.014092008272857	1.9345285270281751
9	79.17745050424611	5.3978047255716755	10.945209439984376	4.47953533019783
10	44.587517420045316	24.68276179522523	16.486876159487093	14.242844625242359
11	35.23534655587158	24.34526624930415	24.51442614344607	15.904961051378198
12	30.59869795067987	21.80317599603349	28.353239843114974	19.244886210171657
13	24.150332930511297	27.105432010783627	30.087318967845672	18.656916090859404
14	20.306383566889803	29.06164445227827	30.90826436115511	19.72370761967682
15	19.996468438491867	22.65683747081865	38.955184040638954	18.391510050050535
16	22.680233273269312	21.113887469360375	36.21835067835146	19.98752857901885
17	22.296326541998955	22.504828158148015	29.757336708077446	25.441508591775587
18	24.66561121375394	22.51234144430087	31.93527034515466	20.886776996790527
19	27.999132643421344	25.673279203857962	26.292316919920417	20.03527123280028
20	29.055272424781542	24.230855069027125	26.06530155223858	20.64857095395275
21	26.10508709705644	27.452976973204514	27.20893783056874	19.232998099170302
22	24.774823326819707	23.729779115727432	27.40862639376213	24.086771163690727
23	23.081449094157644	26.952883770414264	26.306455846604692	23.6592112888234
24	23.644977257251114	24.125066731929753	31.50584007415645	20.72411593666268
25	22.80910039652398	24.127571160647374	29.6005087477476	23.46281969508105
26	20.78444413729088	27.390176045488033	28.463434706690183	23.3619451105309
27	22.18980906742683	25.862189213076796	28.971231405409313	22.976770314087062
28	21.419903297349872	26.37061994438266	31.012943141225684	21.196533617041784
29	23.110139068707575	24.814767379784254	29.842265373071115	22.23282817843706
30	25.40987036569732	24.973719349279673	28.48553074233803	21.130879542684976
31	28.090560142429084	24.53836087359546	25.39636547159978	21.974713512375683
32	30.482099357978605	24.30139119430182	25.149314674176992	20.06719477354258
33	28.506073398148374	25.090825168050333	24.6438638960288	21.759237537772492
34	24.725780906235457	25.447912320901644	27.462931284816317	22.363375488046582
35	25.23151699904768	25.907681051175306	27.83082869327152	21.029973256505492
36	27.736389539476768	26.29789640668372	25.079317476601027	20.886396577238482
37	26.406728100197434	27.801219371470815	25.463382716018074	20.328669812313674
38	24.753044307465228	28.272907914375168	25.303701609047895	21.670346169111706
39	24.631690470363417	27.15805671548295	25.329443332069495	22.880809482084143
40	25.351000440018616	24.1175534457769	27.339453438548926	23.191992675655555
41	21.201700982623702	24.369835012040276	28.00918205992115	26.41928194541487
42	23.953180497664857	25.24711420068146	25.808581757868026	24.99112354378566
43	23.118349790705846	24.517469499862415	27.611485119889224	24.75269558954252
44	23.54841409429079	25.735129082694336	27.6830356973027	23.033421125712177
45	22.58046824574596	26.11279059298532	27.098806370252206	24.20793479101652
46	24.177184210559684	26.275736967777195	26.558991025902767	22.98808779576035
47	23.1264654078161	25.347703470567573	28.020245928559746	23.505585193056582
48	23.870819664647485	23.203849085027574	29.90696839854781	23.01836285177713
49	24.139269061872703	22.731209493243114	28.72725566603221	24.40226577885197
50	22.398722804757274	25.33394496343534	28.501920484705234	23.765411747102156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1319.0
1	2418.5
2	3518.0
3	19114.5
4	34711.0
5	23876.0
6	13041.0
7	13074.0
8	13107.0
9	13737.5
10	14368.0
11	14654.0
12	14940.0
13	14814.0
14	14688.0
15	14366.0
16	14044.0
17	14129.5
18	14215.0
19	14120.5
20	14026.0
21	13975.5
22	13925.0
23	15182.5
24	16440.0
25	18958.0
26	21476.0
27	25912.5
28	30349.0
29	35747.5
30	41146.0
31	46242.5
32	51339.0
33	56662.5
34	61986.0
35	71128.5
36	80271.0
37	90373.5
38	100476.0
39	119169.5
40	137863.0
41	155219.0
42	172575.0
43	183189.0
44	193803.0
45	204679.0
46	215555.0
47	231631.0
48	247707.0
49	265236.5
50	282766.0
51	270608.5
52	258451.0
53	230308.5
54	202166.0
55	185099.5
56	168033.0
57	161783.5
58	155534.0
59	148807.0
60	142080.0
61	131246.0
62	120412.0
63	106022.0
64	91632.0
65	78771.0
66	65910.0
67	55691.0
68	45472.0
69	38982.5
70	32493.0
71	27449.0
72	22405.0
73	17749.5
74	13094.0
75	10556.5
76	8019.0
77	6418.5
78	4818.0
79	3492.0
80	2166.0
81	1650.5
82	1135.0
83	818.5
84	502.0
85	375.0
86	248.0
87	166.0
88	84.0
89	72.0
90	60.0
91	41.0
92	22.0
93	20.5
94	19.0
95	10.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3154412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.57761039987221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08384793117996	27.691080930869532
2	6.7368806960170415	4.658904720345944
3	2.4323976063114308	2.523194903058552
4	1.4144548583977024	1.956338760875287
5	0.9622866362836374	1.6636786201209575
6	0.753973062312047	1.5642352080374722
7	0.6412353715318799	1.5520670796012657
8	0.5098893746174704	1.4104604914045908
9	0.43426618469856276	1.3514298249911265
>10	5.5661126448790705	43.02419970312342
>50	0.42483325327413957	9.246189892250536
>100	0.03679882466076296	1.9050118986746405
>500	0.0018897223976897446	0.4523850339815377
>1k	0.0010078519454345303	0.5163331543363665
>5k	0.0	0.0
>10k+	1.2598149317931629E-4	0.4844897783286146
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11122	0.3525855214854623	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3331	0.1055981273213518	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04108531162067606	0.0
2	0.0	0.0	0.0	0.15666945218316441	0.0
3	0.0	0.0	0.0	0.2721901894869789	0.0
4	0.0	0.0	0.0	0.44629553780546105	0.0
5	0.0	0.0	0.0	0.7213705755621016	0.0
6	0.0	0.0	0.0	1.3125742610667217	0.0
7	0.0	0.0	0.0	1.6269910208305065	0.0
8	0.0	0.0	0.0	2.447651099475909	0.0
9	0.0	0.0	0.0	3.2764584968609047	0.0
10	3.17016293369414E-5	0.0	0.0	4.399076594940674	0.0
11	3.17016293369414E-5	0.0	0.0	5.158235512672409	0.0
12	3.17016293369414E-5	0.0	0.0	5.793789777619411	0.0
13	3.17016293369414E-5	0.0	0.0	6.134962712543574	0.0
14	3.17016293369414E-5	0.0	0.0	6.300350112794397	0.0
15	3.17016293369414E-5	0.0	0.0	6.451249868438238	0.0
16	3.17016293369414E-5	0.0	0.0	6.702485280933499	0.0
17	3.17016293369414E-5	0.0	0.0	6.960695051882887	0.0
18	3.17016293369414E-5	0.0	0.0	7.335693625309567	0.0
19	3.17016293369414E-5	0.0	0.0	7.518199905402338	0.0
20	3.17016293369414E-5	0.0	0.0	7.7226120113669365	0.0
21	3.17016293369414E-5	0.0	0.0	7.933491249716271	0.0
22	3.17016293369414E-5	0.0	0.0	8.150045079716918	0.0
23	3.17016293369414E-5	0.0	0.0	8.39617652988893	0.0
24	3.17016293369414E-5	0.0	0.0	8.590856235647088	0.0
25	3.17016293369414E-5	0.0	0.0	8.76030144445304	0.0
26	3.17016293369414E-5	0.0	0.0	8.921282318226028	0.0
27	3.17016293369414E-5	0.0	0.0	9.105944309113712	0.0
28	3.17016293369414E-5	0.0	0.0	9.274882291850272	0.0
29	6.34032586738828E-5	0.0	0.0	9.44622959841644	0.0
30	6.34032586738828E-5	0.0	0.0	9.672801143287561	0.0
31	9.51048880108242E-5	0.0	0.0	9.851154509937192	0.0
32	9.51048880108242E-5	0.0	0.0	10.044946570073915	0.0
33	9.51048880108242E-5	0.0	0.0	10.22732604364934	0.0
34	9.51048880108242E-5	0.0	0.0	10.417757731076346	0.0
35	9.51048880108242E-5	0.0	0.0	10.616970769829686	0.0
36	9.51048880108242E-5	0.0	0.0	10.809748377827626	0.0
37	9.51048880108242E-5	0.0	0.0	11.008327383994228	0.0
38	9.51048880108242E-5	0.0	0.0	11.235881679374794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	160	0.0	41.25	2
CGTTTTT	12645	0.0	40.572556	1
CGTAAGA	185	0.0	40.43243	2
GAGCGAT	2995	0.0	40.32721	7
CTATCTC	1585	0.0	37.892746	6
TGCGTAG	255	0.0	37.09804	1
GTTTTTA	7490	0.0	36.86248	2
AGCGATA	695	0.0	36.719425	8
GTAACGA	90	0.0	36.666668	2
TAATGCG	145	0.0	36.413795	1
TGATTCG	515	0.0	36.31068	15
CCGCAAT	170	0.0	36.235294	33
TAAGCGA	160	0.0	35.75	2
ATTCGGC	535	0.0	35.364487	17
CGCATTA	25	0.0023533655	35.2	30
CGTTAGT	25	0.0023533655	35.2	41
CGTAATT	25	0.0023533655	35.2	13
CTAAGCG	95	0.0	34.736843	1
AGACCGA	1005	0.0	34.587067	8
GACCGAT	2135	0.0	34.51991	9
>>END_MODULE