FastQCFastQC Report
Thu 26 May 2016
SRR1546708_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546708_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences514382
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.36645916847790166No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.32874400737195314No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5760.11197903503621823No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.8524195E-444.0000041
CGTTAAG207.8524195E-444.00000432
GTATGCG207.8524195E-444.0000041
ATAACGC207.8524195E-444.00000411
GCGTGCA207.8524195E-444.00000444
ATTAGCG351.4440047E-744.01
TTGTCGC502.7284841E-1144.038
TAAGTCG254.4393993E-544.01
GCGATCG302.5249792E-644.09
TAGTACG302.5249792E-644.01
CGTTTTT20900.040.421051
GAGCGAT4600.040.173917
TGTCGCA557.8216544E-1140.00000439
TGTTATC501.3442332E-939.624
GCGATAT501.3442332E-939.69
GTTGATC2450.039.51020416
ATTGGGC900.039.1111074
TATGCGA404.1186286E-738.5000042
CGTTAGA404.1186286E-738.5000042
AGCGATA750.038.133338
Produced by FastQC (version 0.11.5)