##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546708_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	514382
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.469182436399407	31.0	31.0	33.0	30.0	34.0
2	31.8665544284209	31.0	31.0	34.0	30.0	34.0
3	31.997768195621152	33.0	31.0	34.0	30.0	34.0
4	35.70053773265783	37.0	35.0	37.0	33.0	37.0
5	35.631340132430765	37.0	35.0	37.0	33.0	37.0
6	35.668592602385	37.0	35.0	37.0	33.0	37.0
7	35.98418684946207	37.0	35.0	37.0	35.0	37.0
8	18.548403715526593	31.0	0.0	37.0	0.0	37.0
9	27.951143313724042	30.0	17.0	39.0	17.0	39.0
10	33.59543102208087	32.0	32.0	39.0	27.0	39.0
11	35.73298443569176	37.0	35.0	39.0	32.0	39.0
12	36.42469993117955	37.0	35.0	39.0	32.0	39.0
13	36.62688041183401	39.0	35.0	39.0	33.0	39.0
14	37.75698022092531	39.0	36.0	41.0	33.0	41.0
15	37.98715546033881	40.0	36.0	41.0	33.0	41.0
16	38.0635325497393	40.0	37.0	41.0	33.0	41.0
17	38.01110653172156	40.0	36.0	41.0	33.0	41.0
18	37.96890832105323	40.0	36.0	41.0	33.0	41.0
19	37.978949496677565	40.0	36.0	41.0	33.0	41.0
20	37.89234071176674	40.0	36.0	41.0	33.0	41.0
21	37.76315073233511	40.0	36.0	41.0	33.0	41.0
22	37.74812687846775	40.0	36.0	41.0	33.0	41.0
23	37.60032816078323	39.0	36.0	41.0	33.0	41.0
24	37.46085593974906	39.0	35.0	41.0	33.0	41.0
25	37.29530582329864	39.0	35.0	41.0	32.0	41.0
26	37.2610180760602	39.0	35.0	41.0	32.0	41.0
27	37.11995170904114	39.0	35.0	41.0	32.0	41.0
28	37.13094354001501	39.0	35.0	41.0	32.0	41.0
29	37.02025343032999	39.0	35.0	41.0	32.0	41.0
30	36.93183470650217	39.0	35.0	40.0	31.0	41.0
31	36.93656854244511	39.0	35.0	41.0	31.0	41.0
32	36.76409944360417	39.0	35.0	41.0	31.0	41.0
33	36.67512665684258	39.0	35.0	41.0	31.0	41.0
34	36.52143154309443	39.0	35.0	41.0	30.0	41.0
35	36.35802963556267	39.0	35.0	41.0	30.0	41.0
36	36.18869439443837	39.0	35.0	41.0	30.0	41.0
37	36.107286024783136	39.0	35.0	41.0	30.0	41.0
38	35.99433494951223	38.0	35.0	40.0	29.0	41.0
39	35.88350875419435	38.0	35.0	40.0	29.0	41.0
40	35.76660147516826	38.0	35.0	40.0	28.0	41.0
41	35.72035374488221	38.0	35.0	40.0	28.0	41.0
42	35.53678589064158	38.0	35.0	40.0	28.0	41.0
43	35.51321780311131	38.0	35.0	40.0	28.0	41.0
44	35.24761169714337	38.0	34.0	40.0	27.0	41.0
45	35.13983382000148	38.0	34.0	40.0	26.0	41.0
46	35.132137205423206	37.0	34.0	40.0	27.0	41.0
47	34.991343009669855	37.0	34.0	40.0	26.0	41.0
48	34.937921622451796	37.0	34.0	40.0	26.0	41.0
49	34.88014549498233	37.0	34.0	40.0	26.0	41.0
50	34.22995944648141	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	7.0
15	8.0
16	29.0
17	78.0
18	162.0
19	330.0
20	576.0
21	1013.0
22	1538.0
23	2320.0
24	3394.0
25	4857.0
26	6314.0
27	7697.0
28	8678.0
29	10024.0
30	12343.0
31	15535.0
32	20213.0
33	26678.0
34	39649.0
35	58833.0
36	58060.0
37	83431.0
38	84953.0
39	67657.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13623338297219	25.472703166129453	28.328168559553017	14.062894891345342
2	31.79679693301865	25.725822443242567	28.493998623591025	13.983382000147751
3	29.057004327523124	25.5032252294987	31.106259550295306	14.333510892682868
4	26.187541554720035	28.044721627117593	30.411834006633203	15.355902811529173
5	23.854839399512425	31.309027143251512	29.80897465307884	15.027158804157223
6	21.92961651068661	39.8579265992978	27.161137053784927	11.051319836230661
7	85.64490981410702	4.289030331543484	7.778460366031472	2.287599488318021
8	44.41718411608493	50.24417650695398	3.822645426939512	1.5159939500215793
9	80.23433945977891	5.319004164220365	10.165402366334748	4.281254009665968
10	46.34357345319238	23.950099342511987	15.46457690976745	14.241750294528192
11	36.721541578049	23.488185822987585	23.579946421142264	16.210326177821152
12	31.43655882204276	21.375553576913656	27.624217021590958	19.563670579452623
13	23.830538393645188	28.285398789226683	29.24305282844267	18.641009988685454
14	20.314085640632836	29.718380503205786	30.58388512817323	19.38364872798815
15	19.163967634948346	22.128690350750997	38.96209431900805	19.745247695292605
16	22.04781660322484	20.29270075546967	36.73942711836729	20.920055522938206
17	22.523727502128768	21.471979968194844	28.787360366420288	27.216932163256104
18	24.638498236719013	21.843882561987005	31.510239471832218	22.00737972946176
19	28.69909911311049	23.96720725064252	25.836440621950224	21.497253014296767
20	30.16007558584865	23.365319937322845	25.304929021622062	21.16967545520644
21	26.137967502750875	26.325571268045927	26.87380196041075	20.662659268792456
22	25.703465517844716	23.103841114191397	26.251501802162597	24.94119156580129
23	23.832676882161508	27.03554945546306	25.44820775221528	23.683565910160155
24	23.449498621646946	23.53989836347306	31.773079151292233	21.23752386358776
25	23.06981970597727	24.19621992993534	29.87390694075609	22.860053423331298
26	21.70390876819173	27.580669619076875	27.575031785715677	23.14038982701572
27	22.80406390581319	25.399216924386934	27.79646255117792	24.000256618621957
28	21.569767215804596	25.611121695549222	31.301250821373998	21.517860267272184
29	22.382198443957993	23.9707065954874	30.6484285997566	22.998666360798005
30	24.311309493722565	25.17059306118799	28.510912123674625	22.007185321414823
31	27.419505348165373	24.676602213918837	25.196643739477665	22.707248698438125
32	29.146626437161487	24.584452799670284	24.939830709472727	21.329090053695502
33	27.617218331901196	24.92038990477894	24.84359872623848	22.618793037081392
34	23.033465401199887	25.99507758825153	27.327550341963754	23.643906668584826
35	23.336741954422976	26.238476463017758	28.47786275569518	21.94691882686408
36	27.212655186223465	25.596735500075816	24.994264962615333	22.196344351085383
37	24.391016792967093	28.15747829434156	25.792504403342264	21.659000509349084
38	23.86106045701444	28.333028760726464	24.382074022807952	23.42383675945115
39	24.03661092339934	27.27467135319665	24.894922450630077	23.79379527277393
40	25.70832571901816	24.091045176541947	26.07808982429401	24.122539280145883
41	22.273913161813592	23.37056895459017	26.68250444222387	27.673013441372362
42	24.88617408851787	25.051226520368132	24.853124720538432	25.209474670575567
43	23.712532709153898	24.26329070612891	27.232873623105007	24.79130296161219
44	23.43452920203273	25.803974478111595	27.436418848248966	23.325077471606708
45	21.715378842961066	27.21498808278672	26.71963637918901	24.349996695063204
46	23.829177537316625	26.31040744038477	26.563526717497894	23.29688830480071
47	23.54281448417713	25.02673110645396	27.216543347162226	24.213911062206687
48	24.05060830277887	23.075846355432343	28.77977845258971	24.093766889199077
49	23.80215481879226	22.983113717042976	28.928889424591063	24.2858420395737
50	22.14502062669378	26.496261533257385	27.747860539443447	23.610857300605385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	225.0
1	396.5
2	568.0
3	3079.0
4	5590.0
5	3800.5
6	2011.0
7	2047.0
8	2083.0
9	2184.0
10	2285.0
11	2340.5
12	2396.0
13	2321.0
14	2246.0
15	2153.0
16	2060.0
17	2033.0
18	2006.0
19	1973.0
20	1940.0
21	1983.5
22	2027.0
23	2210.5
24	2394.0
25	2919.0
26	3444.0
27	3959.5
28	4475.0
29	5271.0
30	6067.0
31	6862.0
32	7657.0
33	8475.0
34	9293.0
35	10861.5
36	12430.0
37	14465.5
38	16501.0
39	19602.0
40	22703.0
41	26203.0
42	29703.0
43	32114.0
44	34525.0
45	36963.0
46	39401.0
47	40571.5
48	41742.0
49	41068.0
50	40394.0
51	37095.5
52	33797.0
53	31682.5
54	29568.0
55	28244.0
56	26920.0
57	26315.0
58	25710.0
59	24668.0
60	23626.0
61	22439.5
62	21253.0
63	19204.0
64	17155.0
65	14873.5
66	12592.0
67	11119.5
68	9647.0
69	8261.5
70	6876.0
71	5719.0
72	4562.0
73	3785.5
74	3009.0
75	2335.0
76	1661.0
77	1329.0
78	997.0
79	745.5
80	494.0
81	347.0
82	200.0
83	143.0
84	86.0
85	57.5
86	29.0
87	28.0
88	27.0
89	16.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	514382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.68690232620627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.86619204256765	27.040706119514564
2	9.985496797727563	8.325288593710289
3	6.229497190849144	7.790653228089138
4	4.634149047751702	7.727332748748279
5	3.50878372753127	7.3135162266688996
6	2.655100055215407	6.640973800084173
7	2.008946591638053	5.862273224092636
8	1.6009947920574308	5.339241081701043
9	1.2024182831986787	4.511258417425315
>10	3.2835868074473438	17.717885245855314
>50	0.015931671260720325	0.43911507913808256
>100	0.007497257056904634	0.48072162320683137
>500	4.685785660565396E-4	0.11251347215243189
>1k	9.371571321130793E-4	0.6985211396130147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1885	0.36645916847790166	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1691	0.32874400737195314	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	576	0.11197903503621823	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04160332204470608	0.0
2	0.0	0.0	0.0	0.1112014028484667	0.0
3	0.0	0.0	0.0	0.18041066755835158	0.0
4	0.0	0.0	0.0	0.3063870819740971	0.0
5	0.0	0.0	0.0	0.4936020311752744	0.0
6	0.0	0.0	0.0	0.9271319758467442	0.0
7	0.0	0.0	0.0	1.1611992643599505	0.0
8	0.0	0.0	0.0	1.7533661753327294	0.0
9	0.0	0.0	0.0	2.3744998852992523	0.0
10	0.0	0.0	0.0	3.182070912279201	0.0
11	0.0	0.0	0.0	3.7153321850298027	0.0
12	0.0	0.0	0.0	4.186382882760284	0.0
13	0.0	0.0	0.0	4.42219984369593	0.0
14	0.0	0.0	0.0	4.538261447717844	0.0
15	1.9440804693787885E-4	0.0	0.0	4.639742448219416	0.0
16	1.9440804693787885E-4	0.0	0.0	4.81082152952475	0.0
17	1.9440804693787885E-4	0.0	0.0	4.981511794736208	0.0
18	1.9440804693787885E-4	0.0	0.0	5.2663195835002	0.0
19	1.9440804693787885E-4	0.0	0.0	5.400266727840399	0.0
20	1.9440804693787885E-4	0.0	0.0	5.5497665159356275	0.0
21	1.9440804693787885E-4	0.0	0.0	5.707431442002247	0.0
22	1.9440804693787885E-4	0.0	0.0	5.881232235964711	0.0
23	1.9440804693787885E-4	0.0	0.0	6.063003759851628	0.0
24	1.9440804693787885E-4	0.0	0.0	6.226306519279446	0.0
25	1.9440804693787885E-4	0.0	0.0	6.356171094633949	0.0
26	1.9440804693787885E-4	0.0	0.0	6.476509675688496	0.0
27	3.888160938757577E-4	0.0	0.0	6.614150572920514	0.0
28	3.888160938757577E-4	0.0	0.0	6.745959228744396	0.0
29	3.888160938757577E-4	0.0	0.0	6.874657355817272	0.0
30	3.888160938757577E-4	0.0	0.0	7.047680517591984	0.0
31	3.888160938757577E-4	0.0	0.0	7.19193128841989	0.0
32	3.888160938757577E-4	0.0	0.0	7.336570875341672	0.0
33	3.888160938757577E-4	0.0	0.0	7.484126582967522	0.0
34	3.888160938757577E-4	0.0	0.0	7.631293474499497	0.0
35	3.888160938757577E-4	0.0	0.0	7.799845251194637	0.0
36	3.888160938757577E-4	0.0	0.0	7.9339868035817736	0.0
37	3.888160938757577E-4	0.0	0.0	8.085236264099443	0.0
38	3.888160938757577E-4	0.0	0.0	8.245622902823193	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.8524195E-4	44.000004	1
CGTTAAG	20	7.8524195E-4	44.000004	32
GTATGCG	20	7.8524195E-4	44.000004	1
ATAACGC	20	7.8524195E-4	44.000004	11
GCGTGCA	20	7.8524195E-4	44.000004	44
ATTAGCG	35	1.4440047E-7	44.0	1
TTGTCGC	50	2.7284841E-11	44.0	38
TAAGTCG	25	4.4393993E-5	44.0	1
GCGATCG	30	2.5249792E-6	44.0	9
TAGTACG	30	2.5249792E-6	44.0	1
CGTTTTT	2090	0.0	40.42105	1
GAGCGAT	460	0.0	40.17391	7
TGTCGCA	55	7.8216544E-11	40.000004	39
TGTTATC	50	1.3442332E-9	39.6	24
GCGATAT	50	1.3442332E-9	39.6	9
GTTGATC	245	0.0	39.510204	16
ATTGGGC	90	0.0	39.111107	4
TATGCGA	40	4.1186286E-7	38.500004	2
CGTTAGA	40	4.1186286E-7	38.500004	2
AGCGATA	75	0.0	38.13333	8
>>END_MODULE