FastQCFastQC Report
Thu 26 May 2016
SRR1546707_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546707_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2248754
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110000.48915977470190164No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100000.44469070427445595No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24070.10703705251886156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151300.042.415071
GAGCGAT20550.041.0024347
GTTTTTA84100.039.0297242
CGTTAGG1900.038.2105262
ACGAGTA1900.037.052635
AGCGATA5250.036.0380978
AGCGATC3250.035.8769238
AGACCGA6350.035.3385858
TCCGCGA250.002353222335.22
GTCGAAC250.002353222335.227
ACGGTTC250.002353222335.211
TCGTTAG1450.034.896551
GTTAGCG1900.034.7368431
TATTTTT99050.034.449277
AGGGCGA16350.034.4464846
TTATTTT100050.034.2588736
ACGTTAG900.034.222221
GAGACCG7650.034.222227
GCGATCG1350.034.222229
CGATCGA451.0377425E-634.2222210
Produced by FastQC (version 0.11.5)