FastQCFastQC Report
Thu 26 May 2016
SRR1546706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546706_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3195325
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127850.4001157941680424No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32930.10305680955771324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT151250.041.2654531
GAGCGAT34150.040.2635427
TATCTCT17500.039.9771467
CTATCTC17600.039.3756
GTTTTTA86800.037.866362
AGCGATA7100.037.4929588
CGTTACA301.3016137E-436.66666839
GTATGCG601.9826984E-1036.6666681
ATGTCTC27600.036.586968
CGAATAT2950.035.79660814
ATCTCTT19650.035.4910968
AGCGATG17550.035.350438
ACGTTAC250.0023533735.241
CGTTGAG3650.034.9589043
GGGCGAT40250.034.598767
TTACGGG5500.034.43
ACGTCGA451.0379244E-634.222222
TTATTTT101450.034.133076
GAGACCG12650.034.0869567
TATGCGA2200.034.02
Produced by FastQC (version 0.11.5)