##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546706_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3195325
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477598053406147	31.0	31.0	33.0	30.0	34.0
2	31.871752325663273	31.0	31.0	34.0	30.0	34.0
3	31.988807398308467	31.0	31.0	34.0	30.0	34.0
4	35.688083684758205	37.0	35.0	37.0	33.0	37.0
5	35.63193571858888	37.0	35.0	37.0	33.0	37.0
6	35.663852346787884	37.0	35.0	37.0	33.0	37.0
7	36.01470398159812	37.0	35.0	37.0	35.0	37.0
8	18.611839484246516	32.0	0.0	37.0	0.0	37.0
9	27.998878048398833	30.0	17.0	39.0	17.0	39.0
10	33.511414018918266	32.0	32.0	39.0	27.0	39.0
11	35.732504518319736	37.0	34.0	39.0	32.0	39.0
12	36.50361731592248	37.0	35.0	39.0	32.0	39.0
13	36.72949418290784	39.0	35.0	39.0	33.0	39.0
14	37.87835634872822	40.0	37.0	41.0	33.0	41.0
15	38.081534742162376	40.0	37.0	41.0	33.0	41.0
16	38.135208468622125	40.0	37.0	41.0	33.0	41.0
17	38.090784192531274	40.0	37.0	41.0	33.0	41.0
18	38.06631125159413	40.0	37.0	41.0	33.0	41.0
19	38.0629588539507	40.0	37.0	41.0	33.0	41.0
20	37.982831167408634	40.0	36.0	41.0	33.0	41.0
21	37.83910400350512	40.0	36.0	41.0	33.0	41.0
22	37.82310281426772	40.0	36.0	41.0	33.0	41.0
23	37.67342508195567	39.0	36.0	41.0	33.0	41.0
24	37.52463051489285	39.0	35.0	41.0	33.0	41.0
25	37.36125840094513	39.0	35.0	41.0	32.0	41.0
26	37.32263666450205	39.0	35.0	41.0	32.0	41.0
27	37.18967178612504	39.0	35.0	41.0	32.0	41.0
28	37.17097134094341	39.0	35.0	41.0	32.0	41.0
29	37.06228474411836	39.0	35.0	41.0	32.0	41.0
30	36.9083357717916	39.0	35.0	40.0	31.0	41.0
31	36.94033940209525	39.0	35.0	41.0	31.0	41.0
32	36.707073302402726	39.0	35.0	41.0	31.0	41.0
33	36.59505120762364	39.0	35.0	41.0	30.0	41.0
34	36.42605493963838	39.0	35.0	41.0	30.0	41.0
35	36.260145994538895	39.0	35.0	41.0	30.0	41.0
36	36.11897913357796	39.0	35.0	41.0	30.0	41.0
37	36.07730700319999	39.0	35.0	41.0	30.0	41.0
38	35.959503023949054	39.0	35.0	40.0	29.0	41.0
39	35.87431043790538	39.0	35.0	40.0	29.0	41.0
40	35.77844413322588	38.0	35.0	40.0	28.0	41.0
41	35.74165976856814	38.0	35.0	40.0	28.0	41.0
42	35.56373107586865	38.0	35.0	40.0	27.0	41.0
43	35.57156470781533	38.0	35.0	40.0	28.0	41.0
44	35.32292771470821	38.0	35.0	40.0	27.0	41.0
45	35.22180122522748	38.0	34.0	40.0	26.0	41.0
46	35.20413729432843	38.0	34.0	40.0	26.0	41.0
47	35.06405451714614	38.0	34.0	40.0	26.0	41.0
48	35.025701610947245	38.0	34.0	40.0	26.0	41.0
49	34.985626814173834	37.0	34.0	40.0	26.0	41.0
50	34.37075070610971	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	5.0
12	4.0
13	14.0
14	26.0
15	56.0
16	146.0
17	360.0
18	837.0
19	1861.0
20	3649.0
21	6179.0
22	9543.0
23	14177.0
24	21311.0
25	31362.0
26	42880.0
27	50449.0
28	55311.0
29	62008.0
30	75027.0
31	95197.0
32	122490.0
33	161840.0
34	238014.0
35	345080.0
36	355074.0
37	518576.0
38	533135.0
39	450711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.894290878079694	25.150274228756075	28.369508578939545	15.585926314224688
2	31.751543270246373	25.745769209704804	28.787932369946716	13.714755150102103
3	28.364814220775664	25.4318418314256	32.53474998630812	13.668593961490616
4	25.136817068686284	28.408847300352857	31.46909938738626	14.9852362435746
5	23.18462128265513	31.100936524453694	31.02526347085195	14.689178722039228
6	20.920532340215782	39.654338760532966	27.990267030740224	11.434861868511028
7	85.17383990673875	3.7961083770821435	8.720959526808697	2.3090921893704084
8	44.04781986182939	49.904657585691595	4.169560216879347	1.877962335599665
9	79.68084623629834	4.646287936281912	11.562892663500584	4.109973163919163
10	41.67760712916527	23.536854623551594	19.82956350293006	14.955974744353078
11	34.049932323003134	23.944888235156046	25.11387730512546	16.891302136715357
12	29.69707306768482	21.42076314615884	29.008222950717062	19.873940835439274
13	23.20687254035192	26.28771721186421	31.241329129274796	19.264081118509072
14	20.08177571921479	28.295494198555705	31.63077308255029	19.99195699967922
15	19.579479395679623	22.15164341655387	39.338627526151484	18.930249661615015
16	21.803447223678344	20.54079006047898	36.73732718894009	20.918435526902584
17	21.622777025811146	21.99341225071002	30.58120848426999	25.802602239208845
18	23.87065478472456	22.452708253464046	32.09529547072677	21.581341491084633
19	26.94883931994398	25.23596191310743	26.933942556703933	20.881256210244654
20	28.389099700343472	24.00506990681699	26.32777573486265	21.278054657976888
21	25.267257634199964	26.73947094583493	27.719027016031234	20.27424440393387
22	23.943855476359996	23.762684546955317	27.378060134728077	24.91539984195661
23	22.695782119189754	26.89663805716164	26.652468841197685	23.755110982450926
24	23.143060565044244	23.498329590886684	32.20667694209509	21.15193290197398
25	22.478120378991182	24.55190004146683	29.925187578728295	23.04479200081369
26	20.893336358586375	27.618880708535126	28.363969236306165	23.123813696572338
27	22.336225579557635	25.774310907341192	28.509588226549727	23.379875286551446
28	21.089497938394373	25.992473379077246	31.459116052357743	21.45891263017064
29	21.899838044643346	23.948455947360596	30.35644261538341	23.795263392612647
30	23.312558190481404	25.67876507084569	29.157534836049543	21.851141902623365
31	25.819908772973015	24.578438812953298	25.975354619639628	23.626297794434056
32	26.798682450142003	24.779232159482994	25.831112641124143	22.59097274925086
33	25.253080672545046	24.496976050949435	25.885003872845484	24.364939403660035
34	21.34637321712189	25.597990814705856	27.736458732679775	25.31917723549248
35	22.362545280996457	24.297152871773605	29.4863276818477	23.853974165382237
36	25.11250029339738	24.73109934044268	26.89745174591004	23.258948620249896
37	23.31606331124377	26.370525689875052	28.07914687864302	22.23426412023816
38	23.115645513367188	26.493079733673415	27.418212544889798	22.973062208069603
39	23.12954081353227	25.725646061042305	27.7371785342649	23.407634591160523
40	24.569143983788816	23.49044306917137	28.909923090765417	23.0304898562744
41	21.275519704568392	23.156517725114035	29.18172642845407	26.386236141863506
42	23.136425872172627	24.9380579440276	27.22352186397315	24.70199431982662
43	22.41117883157425	24.106468043156802	28.872117859685638	24.610235265583313
44	23.00664251680189	25.413533834586467	28.180513719261736	23.39930992934991
45	21.819220267108978	26.574323425629633	27.299820831996747	24.306635475264645
46	23.51751386791641	25.926940139109476	27.07148725090562	23.484058742068488
47	22.87419902513829	24.926228161454624	28.4904352452411	23.709137568165993
48	23.068451565959645	22.9768489903218	29.514055690735685	24.440643752982876
49	22.861586849537996	22.81605157534836	29.11609930132303	25.206262273790614
50	21.608318402666395	25.139039064883857	29.20066033971505	24.051982192734698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1164.0
1	2005.5
2	2847.0
3	21473.5
4	40100.0
5	27763.5
6	15427.0
7	15523.0
8	15619.0
9	16295.0
10	16971.0
11	16819.0
12	16667.0
13	16438.5
14	16210.0
15	15716.5
16	15223.0
17	14910.5
18	14598.0
19	14317.5
20	14037.0
21	14546.5
22	15056.0
23	15993.5
24	16931.0
25	19415.0
26	21899.0
27	25505.5
28	29112.0
29	32983.0
30	36854.0
31	42898.5
32	48943.0
33	56504.0
34	64065.0
35	72588.5
36	81112.0
37	89849.5
38	98587.0
39	117370.5
40	136154.0
41	157802.0
42	179450.0
43	197912.5
44	216375.0
45	240270.5
46	264166.0
47	278524.0
48	292882.0
49	281378.0
50	269874.0
51	241931.0
52	213988.0
53	198312.0
54	182636.0
55	173263.5
56	163891.0
57	159132.5
58	154374.0
59	145926.5
60	137479.0
61	127476.5
62	117474.0
63	103741.0
64	90008.0
65	76430.0
66	62852.0
67	54203.0
68	45554.0
69	39398.5
70	33243.0
71	27420.5
72	21598.0
73	17708.5
74	13819.0
75	11338.0
76	8857.0
77	6909.5
78	4962.0
79	3655.0
80	2348.0
81	1678.0
82	1008.0
83	773.5
84	539.0
85	374.0
86	209.0
87	153.5
88	98.0
89	66.5
90	35.0
91	30.5
92	26.0
93	14.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3195325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.10093163587281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16246906268476	26.995139456335092
2	6.530557067468727	4.4539616020383415
3	2.458823222044875	2.515444877989464
4	1.435254149854187	1.9577401457712154
5	1.0339703506405757	1.762967612035686
6	0.8237699346454355	1.6854793335018845
7	0.6725131763798156	1.6053328096366237
8	0.5547019760298588	1.513268333030222
9	0.5005673822421861	1.5362832672889741
>10	6.5716808975568375	48.33304907781103
>50	0.22932361955243552	4.843031169019187
>100	0.023758211710556917	1.3266677383934589
>500	0.0013676400518213203	0.3427878293358569
>1k	0.001118978224217444	0.5867883188631119
>5k	0.0	0.0
>10k+	1.2433091380193822E-4	0.542058428949775
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12785	0.4001157941680424	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3293	0.10305680955771324	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04835188908796445	0.0
2	0.0	0.0	0.0	0.15212850023080593	0.0
3	0.0	0.0	0.0	0.2519305547948957	0.0
4	0.0	0.0	0.0	0.40912896184269204	0.0
5	0.0	0.0	0.0	0.6626555984133069	0.0
6	0.0	0.0	0.0	1.2898218491076807	0.0
7	3.129572109253362E-5	0.0	0.0	1.6409285440448154	0.0
8	3.129572109253362E-5	0.0	0.0	2.524625820534687	0.0
9	3.129572109253362E-5	0.0	0.0	3.488346255858168	0.0
10	3.129572109253362E-5	0.0	0.0	4.667850688114667	0.0
11	3.129572109253362E-5	0.0	0.0	5.3544475131637626	0.0
12	3.129572109253362E-5	0.0	0.0	5.946124416139203	0.0
13	3.129572109253362E-5	0.0	0.0	6.258612191248152	0.0
14	3.129572109253362E-5	0.0	0.0	6.4155915282483	0.0
15	3.129572109253362E-5	0.0	0.0	6.546970965394756	0.0
16	3.129572109253362E-5	0.0	0.0	6.765258620015178	0.0
17	3.129572109253362E-5	0.0	0.0	6.9864880724182985	0.0
18	3.129572109253362E-5	0.0	0.0	7.32482611314968	0.0
19	3.129572109253362E-5	0.0	0.0	7.487595158551947	0.0
20	6.259144218506725E-5	0.0	0.0	7.676652609671943	0.0
21	6.259144218506725E-5	0.0	0.0	7.87672615461651	0.0
22	6.259144218506725E-5	0.0	0.0	8.081181100514032	0.0
23	6.259144218506725E-5	0.0	0.0	8.31189315640819	0.0
24	6.259144218506725E-5	0.0	0.0	8.498071401187673	0.0
25	6.259144218506725E-5	0.0	0.0	8.661528952453976	0.0
26	6.259144218506725E-5	0.0	0.0	8.812249145235617	0.0
27	6.259144218506725E-5	0.0	0.0	8.972796194440315	0.0
28	6.259144218506725E-5	0.0	0.0	9.129431278508445	0.0
29	6.259144218506725E-5	0.0	0.0	9.296988569237872	0.0
30	9.388716327760088E-5	0.0	0.0	9.512553496123243	0.0
31	9.388716327760088E-5	0.0	0.0	9.694412931391955	0.0
32	9.388716327760088E-5	0.0	0.0	9.889604343846088	0.0
33	9.388716327760088E-5	0.0	0.0	10.075313152809182	0.0
34	9.388716327760088E-5	0.0	0.0	10.252040089818719	0.0
35	9.388716327760088E-5	0.0	0.0	10.451800677552361	0.0
36	1.251828843701345E-4	0.0	0.0	10.63112515941258	0.0
37	1.251828843701345E-4	0.0	0.0	10.8225923810567	0.0
38	1.251828843701345E-4	0.0	0.0	11.033807202710209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15125	0.0	41.265453	1
GAGCGAT	3415	0.0	40.263542	7
TATCTCT	1750	0.0	39.977146	7
CTATCTC	1760	0.0	39.375	6
GTTTTTA	8680	0.0	37.86636	2
AGCGATA	710	0.0	37.492958	8
CGTTACA	30	1.3016137E-4	36.666668	39
GTATGCG	60	1.9826984E-10	36.666668	1
ATGTCTC	2760	0.0	36.58696	8
CGAATAT	295	0.0	35.796608	14
ATCTCTT	1965	0.0	35.491096	8
AGCGATG	1755	0.0	35.35043	8
ACGTTAC	25	0.00235337	35.2	41
CGTTGAG	365	0.0	34.958904	3
GGGCGAT	4025	0.0	34.59876	7
TTACGGG	550	0.0	34.4	3
ACGTCGA	45	1.0379244E-6	34.22222	2
TTATTTT	10145	0.0	34.13307	6
GAGACCG	1265	0.0	34.086956	7
TATGCGA	220	0.0	34.0	2
>>END_MODULE