FastQCFastQC Report
Thu 26 May 2016
SRR1546705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546705_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1610214
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81440.5057712825748627No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.46639763410329316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG950.044.01
CGTTTTT92550.041.2901151
GAGCGAT16600.040.819287
GTTTTTA50650.040.0039522
ATACGAG1900.038.2105263
CTATCTC9300.038.0860256
ATCGACG357.2920266E-637.7142871
AGCGATA3750.037.5466658
GACCGAT11300.036.7964639
CGTTAGA1200.036.6666642
TATCTCT9750.036.553857
TATGCGA1150.036.3478242
TGATTCG3150.036.3174615
CGCATGA850.036.2352942
ATGTCTC13800.036.0289888
GTTCACG553.426976E-936.01
CGTAAGA800.035.752
AGACCGA6600.035.6666688
TATAACG505.9480953E-835.21
GAGACCG7600.035.0263147
Produced by FastQC (version 0.11.5)