FastQCFastQC Report
Thu 26 May 2016
SRR1546704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546704_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1448523
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98480.6798649382854122No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92640.6395480085576826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG207.8571617E-444.01
GTCTACG207.8571617E-444.01
TCGACAG502.7284841E-1144.01
CGTTTTT164250.043.07581
GAGCGAT15000.042.5333337
GCGATCG1050.041.904769
GTTTTTA88800.041.252
GAGACCG4100.040.7804877
AGCGATA3500.040.228578
AGACCGA3800.039.9473728
GTTAGCG501.3478711E-939.61
CTAGACG452.3515895E-839.111111
CGAGATC1250.038.726
CTACGCG404.1255225E-738.51
TGCGACG357.291521E-637.7142871
GCTAGCG357.291521E-637.7142871
AGTGCGA1000.037.3999982
GCGATGC2650.037.358499
AGCGATG6850.037.2554748
CGTTGAG1550.036.9032253