FastQCFastQC Report
Thu 26 May 2016
SRR1546703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546703_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1670429
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108060.6468996886428576No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101270.6062514479813269No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC17860.10691864185787005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT132150.042.1021581
GTTTTTA71700.041.361232
CAATCGA800.041.2500042
CTATCTC8100.040.469146
TATCTCT8500.038.5647057
CCGGTAT404.1260319E-738.50000442
TACGTAG800.038.5000041
GAGCGAT18800.037.9148947
TTATTTT81300.037.234936
GACCGAT10600.037.1509449
TATTTTT82000.036.9439057
TTGATCC10900.036.93578317
GTTGATC10850.036.9032316
ATCGCTA301.3013478E-436.66666442
AATCGTT1450.036.4137922
TTTTATT84950.035.9199524
ATGTCTC15950.035.862078
CATATGC10750.035.81395333
TCGACAG800.035.751
TTTATTT85200.035.6854485