##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546703_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1670429
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520111300749686	31.0	31.0	33.0	30.0	34.0
2	31.904919035768657	31.0	31.0	34.0	30.0	34.0
3	32.010617631758066	33.0	31.0	34.0	30.0	34.0
4	35.72704616598491	37.0	35.0	37.0	35.0	37.0
5	35.68027075679361	37.0	35.0	37.0	33.0	37.0
6	35.70806780773083	37.0	35.0	37.0	33.0	37.0
7	36.00136731342667	37.0	35.0	37.0	35.0	37.0
8	18.19699789694743	25.0	0.0	37.0	0.0	37.0
9	27.764080364984085	25.0	17.0	39.0	17.0	39.0
10	33.575392908049366	32.0	32.0	39.0	27.0	39.0
11	35.79545194677535	37.0	35.0	39.0	32.0	39.0
12	36.57443566892098	37.0	35.0	39.0	33.0	39.0
13	36.828270462258494	39.0	35.0	39.0	33.0	39.0
14	37.95611905684109	40.0	37.0	41.0	33.0	41.0
15	38.159439281765344	40.0	37.0	41.0	33.0	41.0
16	38.21223230679065	40.0	37.0	41.0	33.0	41.0
17	38.158983710172656	40.0	37.0	41.0	33.0	41.0
18	38.11386595898419	40.0	37.0	41.0	33.0	41.0
19	38.08247761503183	40.0	37.0	41.0	34.0	41.0
20	37.9787892810769	40.0	36.0	41.0	33.0	41.0
21	37.84767386102612	40.0	36.0	41.0	33.0	41.0
22	37.837666252202276	40.0	36.0	41.0	33.0	41.0
23	37.662047294437535	39.0	36.0	41.0	33.0	41.0
24	37.450945236223745	39.0	35.0	41.0	33.0	41.0
25	37.287631500650434	39.0	35.0	41.0	32.0	41.0
26	37.27382786098661	39.0	35.0	41.0	33.0	41.0
27	37.15668968869674	39.0	35.0	41.0	32.0	41.0
28	37.11869645462333	39.0	35.0	41.0	32.0	41.0
29	36.9826032713752	39.0	35.0	41.0	32.0	41.0
30	36.8445291598745	39.0	35.0	40.0	31.0	41.0
31	36.80008369107576	39.0	35.0	41.0	31.0	41.0
32	36.515073074042654	39.0	35.0	41.0	30.0	41.0
33	36.27407689880863	39.0	35.0	41.0	30.0	41.0
34	36.000041905402746	39.0	35.0	41.0	29.0	41.0
35	35.73592651947494	39.0	35.0	41.0	27.0	41.0
36	35.56427779929587	39.0	35.0	41.0	26.0	41.0
37	35.472092498394126	39.0	35.0	41.0	25.0	41.0
38	35.33680689212172	38.0	35.0	40.0	25.0	41.0
39	35.22077621976151	38.0	35.0	40.0	24.0	41.0
40	35.09204282253241	38.0	35.0	40.0	24.0	41.0
41	35.01264106406199	38.0	34.0	40.0	24.0	41.0
42	34.83596309690505	38.0	34.0	40.0	23.0	41.0
43	34.81479009284441	38.0	34.0	40.0	23.0	41.0
44	34.547497080091404	38.0	34.0	40.0	23.0	41.0
45	34.420614704366365	37.0	34.0	40.0	23.0	41.0
46	34.419727507125415	37.0	34.0	40.0	23.0	41.0
47	34.2877703871281	37.0	33.0	40.0	23.0	41.0
48	34.23287490818227	37.0	33.0	40.0	23.0	41.0
49	34.17304955792793	37.0	33.0	40.0	23.0	41.0
50	33.56438435874856	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	3.0
13	9.0
14	17.0
15	31.0
16	82.0
17	212.0
18	506.0
19	1077.0
20	2073.0
21	3720.0
22	5982.0
23	9109.0
24	13618.0
25	20964.0
26	29319.0
27	34006.0
28	34507.0
29	35974.0
30	41135.0
31	50502.0
32	63611.0
33	83826.0
34	124707.0
35	175945.0
36	187444.0
37	265347.0
38	270541.0
39	216155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.05444290059619	22.792468282099986	26.192253606708217	17.9608352105956
2	35.38462275259828	23.629079715450345	26.98097315120846	14.005324380742913
3	28.804516684037452	24.434621285909188	33.21667667407594	13.544185355977417
4	25.058772327348244	26.81161545926226	32.905199801967036	15.224412411422456
5	22.90118286978974	29.71721635579842	32.53912617656901	14.84247459784283
6	20.980299072872896	38.33033310604641	29.885017561356992	10.8043502597237
7	81.82891939735242	4.142887844978746	11.632520747664223	2.3956720100046156
8	41.371288453445196	51.16003134524125	5.703923962048073	1.764756239265482
9	76.32883528722262	4.843306719411601	14.392829626401364	4.435028366964414
10	41.75981140174171	24.04963036441537	19.733074557493914	14.457483676349009
11	33.48786449468969	23.656857010983405	27.051254498096	15.80402399623091
12	28.1313363213881	21.326198240092815	30.756051289818366	19.78641414870072
13	23.24378946965121	24.58422357370472	33.361968691874964	18.81001826476911
14	20.351658166854143	26.12149334093218	34.326511333316176	19.200337158897504
15	19.805091985352266	21.386063101155454	39.26697872223243	19.54186619125985
16	22.782051796275088	20.636076121762734	36.831257120176915	19.750614961785264
17	22.244944262821107	21.833732532181852	32.56187482377281	23.359448381224222
18	25.177663941418643	21.150494872873974	33.26253315764992	20.409308028057463
19	27.215044757963376	24.491313309335506	28.60151494017405	19.692126992527072
20	28.798410468209063	23.35615581386578	27.7395806705942	20.105853047330953
21	25.427300412049842	26.155975500904262	29.400351646193883	19.01637244085202
22	24.912642201494346	21.983155225394196	29.755110812851072	23.349091760260386
23	23.188953256917834	26.062466587924423	28.561525212984208	22.187054942173535
24	22.698360720509523	22.894118816184346	33.265346806119865	21.142173657186266
25	23.404167432438015	23.45038310517837	30.802326827419783	22.343122634963834
26	21.376065669357992	25.20825488542165	30.677748051548438	22.737931393671925
27	22.97918678375435	23.100892046294693	30.783170071879738	23.136751098071215
28	21.62779741012638	25.281888664528694	31.921799729291102	21.168514196053827
29	22.773311526559944	24.11009387408863	31.01586478683021	22.100729812521212
30	24.17456832945309	23.581307556322358	31.24442882636736	20.999695287857193
31	26.41477129527804	23.16093650194052	28.76261128129361	21.661680921487832
32	26.864176807275257	24.10368833395493	28.715377905915183	20.316756952854625
33	25.255069206772628	24.45210182533948	28.441196842248306	21.85163212563958
34	22.79025328224067	25.947526054684154	31.035141272092382	20.227079390982798
35	22.36964276841458	27.156915977871556	29.90974174897586	20.563699504738004
36	26.29288643815451	27.235877729613172	26.137477258835908	20.333758573396416
37	24.550579521787515	28.45173305779533	26.094434423731865	20.903252996685282
38	24.695572215281224	27.952699575977192	25.418320682890442	21.93340752585114
39	24.77106180508121	25.91879092137409	25.171976779617687	24.138170493927007
40	26.178604418385937	23.3642974349703	27.51861946841201	22.93847867823176
41	22.469676951250246	23.897034833566707	27.61584000277773	26.017448212405313
42	24.641574110602726	24.698924647500732	26.348441029220638	24.311060212675905
43	23.743541329802103	23.541736883159956	28.52195453982181	24.192767247216135
44	24.081957389389196	24.78878180395575	28.262200907671026	22.86705989898403
45	22.585814781711765	25.372105010150086	28.218619288817425	23.823460919320723
46	23.93343266909279	25.102174351618658	27.55998608740629	23.404406891882264
47	23.05335934661096	25.257463801215135	28.46382575972999	23.225351092443915
48	23.503842426107305	23.842916999166082	29.147362743343173	23.505877831383433
49	24.786267479791118	22.82024557763305	28.74806412005539	23.64542282252044
50	21.867556178682243	24.969154630337474	29.342821514712686	23.820467676267594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	796.0
1	1464.5
2	2133.0
3	18183.5
4	34234.0
5	23552.5
6	12871.0
7	12854.0
8	12837.0
9	13223.0
10	13609.0
11	13352.5
12	13096.0
13	12712.0
14	12328.0
15	11817.5
16	11307.0
17	10751.0
18	10195.0
19	9660.5
20	9126.0
21	9307.5
22	9489.0
23	9774.5
24	10060.0
25	10637.0
26	11214.0
27	12639.5
28	14065.0
29	15884.5
30	17704.0
31	20351.5
32	22999.0
33	26472.5
34	29946.0
35	34009.5
36	38073.0
37	41996.5
38	45920.0
39	54477.5
40	63035.0
41	75068.0
42	87101.0
43	99280.0
44	111459.0
45	121717.5
46	131976.0
47	131830.0
48	131684.0
49	123874.0
50	116064.0
51	108162.0
52	100260.0
53	96787.0
54	93314.0
55	91048.5
56	88783.0
57	87612.0
58	86441.0
59	82634.5
60	78828.0
61	74037.5
62	69247.0
63	61850.0
64	54453.0
65	47008.0
66	39563.0
67	34342.0
68	29121.0
69	25189.0
70	21257.0
71	18209.0
72	15161.0
73	12298.0
74	9435.0
75	7514.5
76	5594.0
77	4283.0
78	2972.0
79	2190.5
80	1409.0
81	1051.0
82	693.0
83	526.5
84	360.0
85	260.0
86	160.0
87	95.5
88	31.0
89	19.0
90	7.0
91	8.5
92	10.0
93	6.0
94	2.0
95	4.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1670429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.27036764818639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.78658629262293	26.70758314330564
2	7.835522983705953	5.9973669060448245
3	4.424254811902903	5.079535746623454
4	3.2427882375420825	4.964107922237995
5	2.6291932656228587	5.031009644676129
6	2.177102192050304	4.999110077846258
7	1.8027259704897887	4.829368996170446
8	1.4816757883409148	4.536342172417856
9	1.2506318521552418	4.307592669706205
>10	5.304005287775383	29.043474393205273
>50	0.04121101183563308	1.069933597698566
>100	0.022318494042310927	1.6046204674492883
>500	0.0013225412758101445	0.32215900654306606
>1k	3.306353189525361E-4	0.1834129929238705
>5k	0.0	0.0
>10k+	3.306353189525361E-4	1.324382263151218
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10806	0.6468996886428576	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10127	0.6062514479813269	No Hit
TCTGGGAACATGGTCAAGCGAGACACGACCAAAGTGAAACACGTGAGGGC	1786	0.10691864185787005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.98648610626372E-5	0.0	0.0	0.045377564685479	0.0
2	5.98648610626372E-5	0.0	0.0	0.11218674963138212	0.0
3	5.98648610626372E-5	0.0	0.0	0.20892836510860383	0.0
4	5.98648610626372E-5	0.0	0.0	0.3068672778070783	0.0
5	5.98648610626372E-5	0.0	0.0	0.48185226669316683	0.0
6	5.98648610626372E-5	0.0	0.0	1.0039935848814885	0.0
7	5.98648610626372E-5	0.0	0.0	1.3276828886471679	0.0
8	5.98648610626372E-5	0.0	0.0	2.1412463504884074	0.0
9	5.98648610626372E-5	0.0	0.0	3.094893587216218	0.0
10	5.98648610626372E-5	0.0	0.0	4.258846080857073	0.0
11	5.98648610626372E-5	0.0	0.0	4.937174821557815	0.0
12	5.98648610626372E-5	0.0	0.0	5.521695324973405	0.0
13	5.98648610626372E-5	0.0	0.0	5.8716653027455825	0.0
14	5.98648610626372E-5	0.0	0.0	6.088256370070203	0.0
15	5.98648610626372E-5	0.0	0.0	6.233787847313475	0.0
16	5.98648610626372E-5	0.0	0.0	6.424038375770536	0.0
17	5.98648610626372E-5	0.0	0.0	6.61279228270103	0.0
18	5.98648610626372E-5	0.0	0.0	6.913134290652281	0.0
19	5.98648610626372E-5	0.0	0.0	7.060162389422119	0.0
20	5.98648610626372E-5	0.0	0.0	7.211800082493778	0.0
21	5.98648610626372E-5	0.0	0.0	7.382414936522294	0.0
22	5.98648610626372E-5	0.0	0.0	7.552730466245498	0.0
23	5.98648610626372E-5	0.0	0.0	7.745794643172502	0.0
24	5.98648610626372E-5	0.0	0.0	7.912518281231947	0.0
25	5.98648610626372E-5	0.0	0.0	8.061162731250475	0.0
26	5.98648610626372E-5	0.0	0.0	8.202084614191923	0.0
27	5.98648610626372E-5	0.0	0.0	8.368688522529242	0.0
28	5.98648610626372E-5	0.0	0.0	8.514878513244202	0.0
29	5.98648610626372E-5	0.0	0.0	8.675256476031008	0.0
30	5.98648610626372E-5	0.0	0.0	8.885322273499801	0.0
31	5.98648610626372E-5	0.0	0.0	9.05689496530532	0.0
32	5.98648610626372E-5	0.0	0.0	9.275401708183946	0.0
33	5.98648610626372E-5	0.0	0.0	9.456911966925862	0.0
34	5.98648610626372E-5	0.0	0.0	9.63327384761639	0.0
35	5.98648610626372E-5	0.0	0.0	9.81711883593975	0.0
36	5.98648610626372E-5	0.0	0.0	9.994917473295782	0.0
37	5.98648610626372E-5	0.0	0.0	10.186125839529845	0.0
38	5.98648610626372E-5	0.0	0.0	10.423968932531704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13215	0.0	42.102158	1
GTTTTTA	7170	0.0	41.36123	2
CAATCGA	80	0.0	41.250004	2
CTATCTC	810	0.0	40.46914	6
TATCTCT	850	0.0	38.564705	7
CCGGTAT	40	4.1260319E-7	38.500004	42
TACGTAG	80	0.0	38.500004	1
GAGCGAT	1880	0.0	37.914894	7
TTATTTT	8130	0.0	37.23493	6
GACCGAT	1060	0.0	37.150944	9
TATTTTT	8200	0.0	36.943905	7
TTGATCC	1090	0.0	36.935783	17
GTTGATC	1085	0.0	36.90323	16
ATCGCTA	30	1.3013478E-4	36.666664	42
AATCGTT	145	0.0	36.41379	22
TTTTATT	8495	0.0	35.919952	4
ATGTCTC	1595	0.0	35.86207	8
CATATGC	1075	0.0	35.813953	33
TCGACAG	80	0.0	35.75	1
TTTATTT	8520	0.0	35.685448	5
>>END_MODULE