FastQCFastQC Report
Thu 26 May 2016
SRR1546702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546702_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4258890
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502021.1787578453540712No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT327600.042.472531
AGGGCGA45650.038.4446876
CACGACC14050.038.43416627
GGGCGAT91150.037.668687
ACGACCA14700.037.528
TACGGGA12450.036.867474
ACACGAC14650.036.70648226
AACACGT13650.036.59340741
AACGGGA13100.036.4122164
AGGGATT50400.036.3839266
CTACGGG9550.036.2827223
GTTTTTT399000.036.0958632
CGTATCG250.002107747136.00000439
ATAGCGG8250.036.02
CGTAAGG7500.036.02
TAGGGCG19150.035.9530035
TAGGGAT45100.035.9201775
TCAAGCG15200.035.8223717
ATGGGAC27550.035.6896555
ACGGGAA14800.035.5743265
Produced by FastQC (version 0.11.5)