##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546702_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4258890
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33315840512434	31.0	31.0	33.0	30.0	34.0
2	31.7445853262235	31.0	31.0	34.0	30.0	34.0
3	31.768789520274062	31.0	31.0	34.0	30.0	34.0
4	35.59116929528586	37.0	35.0	37.0	33.0	37.0
5	35.52233375363064	37.0	35.0	37.0	33.0	37.0
6	35.59317052095734	37.0	35.0	37.0	33.0	37.0
7	35.96925489975087	37.0	35.0	37.0	35.0	37.0
8	35.94753233823837	37.0	35.0	37.0	35.0	37.0
9	37.67282390482027	39.0	37.0	39.0	35.0	39.0
10	37.11052786054582	39.0	37.0	39.0	33.0	39.0
11	36.89302048186264	39.0	37.0	39.0	32.0	39.0
12	36.562551744703434	39.0	35.0	39.0	32.0	39.0
13	36.4815907431279	39.0	35.0	39.0	32.0	39.0
14	37.588945476403474	40.0	36.0	41.0	32.0	41.0
15	37.71078590900446	40.0	36.0	41.0	33.0	41.0
16	37.72700328019742	40.0	36.0	41.0	33.0	41.0
17	37.62691475947958	40.0	36.0	41.0	32.0	41.0
18	37.45601201251969	39.0	36.0	41.0	32.0	41.0
19	37.34043166177103	39.0	36.0	41.0	32.0	41.0
20	37.15372315321598	39.0	35.0	41.0	32.0	41.0
21	37.066933168032044	39.0	35.0	41.0	32.0	41.0
22	36.98453963356649	39.0	35.0	41.0	32.0	41.0
23	36.891665199148136	38.0	35.0	41.0	31.0	41.0
24	36.76803134149978	38.0	35.0	41.0	31.0	41.0
25	36.66708461594453	38.0	35.0	41.0	31.0	41.0
26	36.51103714817711	38.0	35.0	40.0	31.0	41.0
27	36.41212710354106	38.0	35.0	40.0	31.0	41.0
28	36.314311005919386	38.0	35.0	40.0	30.0	41.0
29	36.30800936394225	38.0	35.0	40.0	30.0	41.0
30	36.246400587946624	38.0	35.0	40.0	30.0	41.0
31	36.131949874263015	38.0	35.0	40.0	30.0	41.0
32	35.943536461378436	38.0	35.0	40.0	29.0	41.0
33	35.68582776263299	38.0	35.0	41.0	27.0	41.0
34	35.40587101333916	38.0	35.0	41.0	26.0	41.0
35	35.21869078562724	38.0	34.0	41.0	25.0	41.0
36	35.076270577544854	38.0	34.0	40.0	24.0	41.0
37	35.035633228376405	38.0	34.0	40.0	24.0	41.0
38	34.94485746286004	38.0	34.0	40.0	23.0	41.0
39	34.84492884296143	38.0	34.0	40.0	23.0	41.0
40	34.72740150602622	38.0	34.0	40.0	23.0	41.0
41	34.6447440530279	38.0	34.0	40.0	23.0	41.0
42	34.56977076186518	38.0	34.0	40.0	23.0	41.0
43	34.44963194635222	37.0	34.0	40.0	23.0	41.0
44	34.30809248419189	37.0	33.0	40.0	22.0	41.0
45	34.19678460819603	37.0	33.0	40.0	22.0	41.0
46	34.148968158369904	37.0	33.0	40.0	22.0	41.0
47	34.10888400498722	37.0	33.0	40.0	22.0	41.0
48	34.00989271852525	37.0	33.0	40.0	22.0	41.0
49	33.92831348074264	36.0	33.0	40.0	21.0	41.0
50	33.839129914132556	36.0	33.0	40.0	20.0	41.0
51	33.48627271425183	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	7.0
12	10.0
13	34.0
14	62.0
15	124.0
16	377.0
17	916.0
18	2150.0
19	4257.0
20	7397.0
21	11527.0
22	17288.0
23	25979.0
24	38634.0
25	59731.0
26	82875.0
27	95424.0
28	96190.0
29	98640.0
30	107232.0
31	124504.0
32	150066.0
33	189179.0
34	292998.0
35	417806.0
36	327631.0
37	412540.0
38	610475.0
39	1084210.0
40	620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.528567302747902	23.555762182164834	26.624049928502497	18.291620586584767
2	35.417679254453624	24.544822712021208	26.638490310855644	13.399007722669523
3	28.3847669228367	24.40288431962319	33.7706773361134	13.44167142142671
4	24.571684171227716	26.761691426639334	33.72961029751884	14.937014104614112
5	22.051966592234127	30.87478192674617	32.57109246775568	14.502159013264018
6	20.3060187044042	39.547534686268015	29.990490479913777	10.155956129414003
7	83.79810232243614	3.547637999572659	10.912420842050393	1.7418388359408201
8	83.8985275506059	3.092261129073538	10.765316784420353	2.2438945359001994
9	78.38800720375497	5.082404100598983	13.179866115349304	3.3497225802967443
10	37.94002662665624	28.50075958759207	19.610320999133577	13.948892786618108
11	29.49709900936629	23.967066536116217	29.73659803375997	16.79923642075752
12	27.420900751134685	22.11977768855265	31.938650681280805	18.52067087903186
13	22.39696258884357	26.421626292296818	33.09005398120167	18.091357137657933
14	19.12021677009737	28.831784807778554	33.61197401200782	18.43602441011625
15	18.601067414279306	23.748723258877313	39.93716672654142	17.713042600301957
16	21.589639553968286	22.36296311949827	37.56124248337008	18.486154843163362
17	21.118155200063864	22.869832280242036	32.64510236235263	23.366910157341465
18	22.626881652261506	22.98551970114279	34.70779945009145	19.679799196504252
19	25.178485473914563	24.959085583332747	30.43647523180923	19.42595371094346
20	26.25947136460439	24.535923679644224	30.06536914548157	19.13923581026981
21	23.845673403163737	25.612589195776366	31.808522878026903	18.733214523032995
22	23.397223220134826	22.80854870635306	31.18880741226	22.60542066125211
23	21.090800654630666	26.5813627494488	30.47984333946169	21.847993256458842
24	21.284935746168603	24.32112592717821	34.94445735860752	19.449480968045666
25	21.624413873098387	25.22206490423561	32.7887078558028	20.364813366863196
26	20.376365672745717	27.386196872894107	31.063540030383503	21.17389742397667
27	19.967338907555725	26.414746565419627	32.27495896818185	21.342955558842796
28	19.593509106833	26.24888175087875	34.107056063903975	20.050553078384272
29	20.44182873941332	24.543273012451603	33.90864755840137	21.10625068973371
30	21.63157536353369	25.65652083054505	33.10064359492731	19.611260210993944
31	24.339722322013483	25.302367518297018	30.26565607470491	20.092254084984585
32	24.97657840423209	24.98545395631256	30.33248099857005	19.7054866408853
33	23.51880419545938	25.817900908452675	29.509614007405688	21.153680888682263
34	20.908922277870527	27.346092526456424	30.9597336395164	20.785251556156652
35	20.906691649702154	27.367882241616947	31.331356292367257	20.39406981631364
36	23.325350032520213	28.048341234453112	28.510104745602728	20.11620398742395
37	21.18946486056226	29.677920772783516	29.42571890797837	19.706895458675852
38	21.27523838371031	29.835544003249677	27.969588319961304	20.919629293078714
39	20.90878139609147	29.30014628224725	28.32094747692474	21.470124844736542
40	23.011794152936563	26.642200197704096	28.867122654024875	21.478882995334462
41	20.10594309784944	26.559056467765075	29.473289988705975	23.861710445679506
42	22.19118127023708	27.291007750845882	27.851130224072467	22.666680754844574
43	21.716268793042317	26.277175508172316	29.23630335603878	22.770252342746584
44	21.295525359894242	27.824714890499635	28.946321694150356	21.933438055455763
45	20.564395887191264	28.71668439429053	28.742536200747143	21.976383517771062
46	22.344765889703655	27.731897278398833	28.693086696298803	21.230250135598713
47	21.024398376102692	27.972499876728445	29.332032524906726	21.671069222262137
48	21.522955511882206	26.60272981927216	30.34969205591128	21.524622612934355
49	21.637727201219096	26.2574520591046	30.376389153042226	21.728431586634077
50	20.62664215323711	27.773809607667726	30.364273320043484	21.235274919051676
51	20.54293489618187	27.963413001979387	29.34250473714982	22.15114736468892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4595.0
1	5576.0
2	6557.0
3	45990.0
4	85423.0
5	59723.0
6	34023.0
7	34240.5
8	34458.0
9	35539.5
10	36621.0
11	36508.0
12	36395.0
13	35644.5
14	34894.0
15	33691.0
16	32488.0
17	30874.0
18	29260.0
19	28032.0
20	26804.0
21	26411.5
22	26019.0
23	27267.0
24	28515.0
25	30873.5
26	37594.0
27	41956.0
28	48634.0
29	55312.0
30	64286.0
31	73260.0
32	82625.5
33	91991.0
34	103797.5
35	115604.0
36	124413.0
37	133222.0
38	154096.5
39	174971.0
40	200281.0
41	225591.0
42	250937.5
43	276284.0
44	302786.5
45	329289.0
46	332907.0
47	336525.0
48	326395.0
49	316265.0
50	298086.5
51	279908.0
52	264219.5
53	248531.0
54	229653.0
55	210775.0
56	201857.0
57	192939.0
58	177103.0
59	161267.0
60	151722.0
61	142177.0
62	128717.0
63	115257.0
64	104470.5
65	93684.0
66	78213.5
67	62743.0
68	53681.0
69	44619.0
70	38024.5
71	31430.0
72	26969.5
73	22509.0
74	18196.5
75	12002.5
76	10121.0
77	7490.5
78	4860.0
79	3756.5
80	2653.0
81	1859.0
82	1065.0
83	791.5
84	518.0
85	361.5
86	205.0
87	146.5
88	88.0
89	73.0
90	58.0
91	38.0
92	18.0
93	14.5
94	11.0
95	12.5
96	14.0
97	7.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4258890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.305767696944372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34424510026108	17.698342992958402
2	6.4872044790961425	2.8940415222659115
3	2.324010203846152	1.555164951969618
4	1.3455863004955002	1.2005734174017364
5	0.913798192302439	1.019148509969295
6	0.6487342161753374	0.868230883383982
7	0.5487799910739439	0.8568671298338619
8	0.4364529180134297	0.7788333919888856
9	0.37927888819581007	0.7614096095205968
>10	5.393468934040403	29.527680279642336
>50	1.7079336845000954	26.816956661976672
>100	0.46537010443254057	13.673077115691576
>500	0.003210617229468648	0.4916150397465419
>1k	0.0018193497633387503	0.6596483632690848
>5k	0.0	0.0
>10k+	1.0702057431404413E-4	1.1984101303815942
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50202	1.1787578453540712	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6960593018368634E-5	0.0	0.0	0.041630565710783794	0.0
2	4.6960593018368634E-5	0.0	0.0	0.12944687465513313	0.0
3	4.6960593018368634E-5	0.0	0.0	0.224589036110348	0.0
4	4.6960593018368634E-5	0.0	0.0	0.3717400543334061	0.0
5	4.6960593018368634E-5	0.0	0.0	0.6389223483114145	0.0
6	4.6960593018368634E-5	0.0	0.0	1.2366602565457196	0.0
7	4.6960593018368634E-5	0.0	0.0	1.551249269175771	0.0
8	4.6960593018368634E-5	0.0	0.0	2.2838110399658125	0.0
9	4.6960593018368634E-5	0.0	0.0	2.677739974500398	0.0
10	4.6960593018368634E-5	0.0	0.0	3.2160257719734484	0.0
11	4.6960593018368634E-5	0.0	0.0	3.5511600440490363	0.0
12	4.6960593018368634E-5	0.0	0.0	3.839286762513237	0.0
13	7.044088952755296E-5	0.0	0.0	3.9960412220085515	0.0
14	7.044088952755296E-5	0.0	0.0	4.080617250034633	0.0
15	7.044088952755296E-5	0.0	0.0	4.1515747060853885	0.0
16	7.044088952755296E-5	0.0	0.0	4.268811826555746	0.0
17	7.044088952755296E-5	0.0	0.0	4.395229742961194	0.0
18	7.044088952755296E-5	0.0	0.0	4.597042891457633	0.0
19	7.044088952755296E-5	0.0	0.0	4.689296976442218	0.0
20	7.044088952755296E-5	0.0	0.0	4.788454268600504	0.0
21	7.044088952755296E-5	0.0	0.0	4.907264568936976	0.0
22	7.044088952755296E-5	0.0	0.0	5.03758021456295	0.0
23	7.044088952755296E-5	0.0	0.0	5.186233971762595	0.0
24	7.044088952755296E-5	0.0	0.0	5.308472395389409	0.0
25	9.392118603673727E-5	0.0	0.0	5.417961018011735	0.0
26	9.392118603673727E-5	0.0	0.0	5.523199706965899	0.0
27	9.392118603673727E-5	0.0	0.0	5.637689632744682	0.0
28	9.392118603673727E-5	0.0	0.0	5.753658817203544	0.0
29	9.392118603673727E-5	0.0	0.0	5.881086386358887	0.0
30	9.392118603673727E-5	0.0	0.0	6.056953807212677	0.0
31	9.392118603673727E-5	2.3480296509184317E-5	0.0	6.189241797745422	0.0
32	9.392118603673727E-5	2.3480296509184317E-5	0.0	6.328996522568087	0.0
33	9.392118603673727E-5	2.3480296509184317E-5	0.0	6.46276377178091	0.0
34	9.392118603673727E-5	2.3480296509184317E-5	0.0	6.600898356144441	0.0
35	9.392118603673727E-5	2.3480296509184317E-5	0.0	6.755821352512039	0.0
36	9.392118603673727E-5	2.3480296509184317E-5	0.0	6.8971492572008195	0.0
37	9.392118603673727E-5	2.3480296509184317E-5	0.0	7.0509451993359775	0.0
38	9.392118603673727E-5	2.3480296509184317E-5	0.0	7.222539206225096	0.0
39	9.392118603673727E-5	2.3480296509184317E-5	0.0	7.426770825261982	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	32760	0.0	42.47253	1
AGGGCGA	4565	0.0	38.444687	6
CACGACC	1405	0.0	38.434166	27
GGGCGAT	9115	0.0	37.66868	7
ACGACCA	1470	0.0	37.5	28
TACGGGA	1245	0.0	36.86747	4
ACACGAC	1465	0.0	36.706482	26
AACACGT	1365	0.0	36.593407	41
AACGGGA	1310	0.0	36.412216	4
AGGGATT	5040	0.0	36.383926	6
CTACGGG	955	0.0	36.282722	3
GTTTTTT	39900	0.0	36.095863	2
CGTATCG	25	0.0021077471	36.000004	39
ATAGCGG	825	0.0	36.0	2
CGTAAGG	750	0.0	36.0	2
TAGGGCG	1915	0.0	35.953003	5
TAGGGAT	4510	0.0	35.920177	5
TCAAGCG	1520	0.0	35.82237	17
ATGGGAC	2755	0.0	35.689655	5
ACGGGAA	1480	0.0	35.574326	5
>>END_MODULE