FastQCFastQC Report
Thu 26 May 2016
SRR1546701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546701_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3947103
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348730.883508740461042No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC61740.15641851758112216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT213200.041.559571
TAACGGG12050.038.2780083
GCGATAT3950.038.1645559
GGCGATA19850.037.8589448
ATTAGCG2800.037.7678571
AGGGCGA45050.037.558276
ACGGGAT9450.037.142865
TATACGG3050.036.8852462
CGCATGG4050.036.6666682
GCGATAA5050.036.5346579
GGGCGAT90300.036.503327
GCGTAAG3350.036.2686581
CGTTCGG7450.036.2416145
TAGGGAC32100.036.1682245
GTTGATC25600.036.03515616
GGGTACC30200.035.9850967
TACGGGA8350.035.8383264
TAAGGGA37550.035.7123834
AGGGACC33400.035.6362276
AGGGATT42600.035.4929586
Produced by FastQC (version 0.11.5)