FastQCFastQC Report
Thu 26 May 2016
SRR1546700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546700_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1021784
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41500.40615237662754555No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25220.24682320333847466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11970.11714804694534266No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT207.032917E-445.00000425
CTCACGA850.045.00000424
AGTTGCA302.165265E-645.00000435
CTATACG253.890501E-545.01
GCGATCG351.2117562E-745.09
TAGTGCG453.8380676E-1045.01
CGAATAT1050.042.85714314
AGGGTAC3850.040.9090926
ACGTTAG556.184564E-1140.909091
GCGTAAG556.184564E-1140.909091
CGTTTTT27750.040.2162131
TTTGTCG451.9279469E-840.01
AGGGCGA10750.039.7674456
TAGCATA5000.039.630
TAGGGTA4900.039.4897965
GTAGCCG403.457717E-739.37500443
GGGCGAT21950.039.362197
TAGGGCG3900.039.230775
GCGATAC750.039.09
ACGGGTA1100.038.8636325
Produced by FastQC (version 0.11.5)