FastQCFastQC Report
Thu 26 May 2016
SRR1546699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546699_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4210432
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292580.6948930656046695No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC88060.2091471848969417No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT43940.10435983766036358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC502.1827873E-1145.019
CGTTTTT199000.041.4949761
GCGTAAT451.9304935E-840.013
GCGTTAG3300.039.5454521
GCGTAAG2750.039.2727281
CGTTAGG7000.039.2142872
TGATTCG6700.038.95522315
TATAGCG2150.038.7209321
ACGGGAT7800.038.3653835
AGGGCGA53150.038.1843836
TCGTTAG2250.038.01
TCACGAC3000.037.50000425
TACGGGA9350.037.2994654
GGGCGAT111400.036.6180427
TAGGGCG20650.036.6101725
TAGGGAC23350.036.2312625
AGGGTAC21100.036.0426526
GGCGATA21950.035.876998
GCGATAC3650.035.753429
CCGTAAG1450.035.6896551
Produced by FastQC (version 0.11.5)