##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546699_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4210432
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300768899723355	31.0	31.0	33.0	30.0	34.0
2	31.695103020307656	31.0	31.0	34.0	30.0	34.0
3	31.773527752021646	31.0	31.0	34.0	30.0	34.0
4	35.552721430960055	37.0	35.0	37.0	33.0	37.0
5	35.472713726287466	37.0	35.0	37.0	33.0	37.0
6	35.53325430739648	37.0	35.0	37.0	33.0	37.0
7	35.926887312275795	37.0	35.0	37.0	35.0	37.0
8	35.90067955022192	37.0	35.0	37.0	35.0	37.0
9	37.59360274670153	39.0	37.0	39.0	35.0	39.0
10	37.05119427175169	39.0	37.0	39.0	33.0	39.0
11	36.795902890724754	39.0	35.0	39.0	32.0	39.0
12	36.249097242278225	38.0	35.0	39.0	32.0	39.0
13	36.07504479350337	38.0	35.0	39.0	32.0	39.0
14	37.08930722548185	39.0	35.0	41.0	32.0	41.0
15	37.28554457119839	39.0	35.0	41.0	32.0	41.0
16	37.357989868973064	39.0	35.0	41.0	32.0	41.0
17	37.27463001421232	39.0	35.0	41.0	32.0	41.0
18	37.15340373624369	39.0	35.0	41.0	32.0	41.0
19	37.07448333092661	38.0	35.0	41.0	32.0	41.0
20	36.91757472867392	38.0	35.0	41.0	32.0	41.0
21	36.79139218968505	38.0	35.0	41.0	31.0	41.0
22	36.700888887411075	38.0	35.0	41.0	31.0	41.0
23	36.62672903873047	38.0	35.0	40.0	31.0	41.0
24	36.50483917089743	38.0	35.0	40.0	31.0	41.0
25	36.375958571472	38.0	35.0	40.0	31.0	41.0
26	36.23148384773819	38.0	35.0	40.0	31.0	41.0
27	36.11596957271843	38.0	35.0	40.0	30.0	41.0
28	36.037460526615796	38.0	35.0	40.0	30.0	41.0
29	36.0429003484678	38.0	35.0	40.0	30.0	41.0
30	35.982369980087554	38.0	35.0	40.0	30.0	41.0
31	35.87040118448653	38.0	35.0	40.0	30.0	41.0
32	35.736486184790536	38.0	35.0	40.0	29.0	41.0
33	35.5803136115249	38.0	34.0	40.0	29.0	41.0
34	35.39369356873594	38.0	34.0	40.0	27.0	41.0
35	35.267871562823004	38.0	34.0	40.0	27.0	41.0
36	35.12880839780811	38.0	34.0	40.0	26.0	41.0
37	35.07358176073144	38.0	34.0	40.0	26.0	41.0
38	34.99251953243753	37.0	34.0	40.0	26.0	41.0
39	34.893705444001945	37.0	34.0	40.0	25.0	41.0
40	34.72738355589165	37.0	34.0	40.0	24.0	41.0
41	34.67220869497477	37.0	34.0	40.0	24.0	41.0
42	34.58869944936767	37.0	34.0	40.0	24.0	41.0
43	34.45465192170305	37.0	34.0	40.0	23.0	41.0
44	34.280326104304734	36.0	33.0	40.0	23.0	41.0
45	34.1640211740743	36.0	33.0	40.0	23.0	41.0
46	34.13694224250623	36.0	33.0	40.0	23.0	41.0
47	34.093437918009364	36.0	33.0	40.0	23.0	41.0
48	33.99553608750836	36.0	33.0	40.0	23.0	41.0
49	33.91692562663404	36.0	33.0	40.0	23.0	41.0
50	33.80124343535295	35.0	33.0	40.0	23.0	41.0
51	33.408611040387306	35.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	6.0
12	12.0
13	14.0
14	44.0
15	126.0
16	414.0
17	1000.0
18	2417.0
19	4596.0
20	7574.0
21	12436.0
22	18460.0
23	26677.0
24	38867.0
25	55321.0
26	71645.0
27	80982.0
28	87311.0
29	96081.0
30	111258.0
31	132441.0
32	161306.0
33	206777.0
34	339836.0
35	522678.0
36	318720.0
37	389682.0
38	554202.0
39	968849.0
40	691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.246414619687485	23.695739534565575	27.18692523712531	14.870920608621633
2	33.19426605155956	25.73144513437101	27.441483439229035	13.632805374840395
3	29.625653614640967	25.09937222593786	31.442545563020612	13.83242859640056
4	25.43444473156199	28.06479240134979	30.747201237307713	15.753561629780508
5	23.16121006110537	31.694063697026813	30.019793693378734	15.124932548489086
6	21.335150407369124	40.08441414087676	27.734565004256094	10.845870447498024
7	83.87051494953486	4.74994014865933	9.141532270322855	2.238012631482945
8	83.45236308293306	4.18512874688393	8.747962204353378	3.6145459658296346
9	77.60320081169819	6.097355330759409	12.22100250045601	4.078441357086398
10	43.8084025582173	24.406117947042013	17.668875782817537	14.116603711923148
11	36.79515546148234	24.210627318051927	24.651223437404997	14.34299378306074
12	31.861766203562958	22.86589594606919	27.663930922052653	17.608406928315194
13	22.396252926065543	32.3134538213656	29.507542218945705	15.782751033623152
14	18.256772701708517	34.319518757220166	30.823036686021766	16.600671855049555
15	17.310694009545813	24.099759834620297	42.60928569799963	15.980260457834255
16	19.374496488721345	21.64901368790661	40.57087253754484	18.4056172858272
17	19.41202232930018	22.591957309843743	30.790498457165437	27.205521903690645
18	22.320037468839303	23.54739371161914	33.92967752477655	20.202891294765003
19	26.940228461117528	26.229303786404817	27.68927273970937	19.141195012768286
20	27.858067770718066	24.43354981151578	27.180655096674165	20.527727321091994
21	23.340787833647475	28.239477564297438	29.02326887122271	19.39646573083237
22	23.21559877941266	24.848827863744148	27.732451206907033	24.20312214993616
23	20.442035401592996	28.963560033744756	26.818126976044265	23.776277588617987
24	20.485522625706814	24.79793047364261	35.24586075728096	19.470686143369612
25	20.325586543138566	25.656654709065485	32.772195347175774	21.245563400620174
26	18.452477085486716	31.134833670274215	29.075068781540708	21.337620462698364
27	19.363286237611725	30.095154131452546	29.47673777892625	21.064821852009487
28	18.859347449382867	28.763628055268438	33.26024502948866	19.116779465860034
29	19.84183570711984	26.277778622241136	32.2765929956831	21.60379267495592
30	21.691574641271966	28.329872089134795	30.12344101507874	19.855112254514502
31	26.509678816805497	26.541385777041405	26.705858211223934	20.243077194929164
32	26.73865769593239	27.00611243691859	26.486118289049678	19.76911157809935
33	25.818277079406577	27.234758808597313	25.353099159421173	21.593864952574936
34	20.44355543564176	28.506267290387306	27.862889128716482	23.187288145254453
35	20.47516739374962	27.10750345807746	29.35741510609838	23.059914042074542
36	25.210097206177416	27.625312557001276	27.010743790660914	20.153846446160394
37	20.65343888703107	29.831736980908374	28.816496739526965	20.698327392533592
38	20.791904488660546	30.520454908189944	26.85700659694777	21.830634006201738
39	20.882417766157964	28.82388315498267	27.784963633185384	22.508735445673985
40	23.07684817139904	25.492491031799112	28.90615499787195	22.524505798929894
41	18.966367346628566	24.810660758801	29.58872153736244	26.634250357208
42	22.488357489207758	26.124017677995987	27.21423834893902	24.173386483857236
43	22.13613709947103	25.05025612573722	29.08155267677996	23.732054098011794
44	21.153625091202045	28.291491229403537	28.599678132790174	21.955205546604244
45	19.22683943120326	31.636421155833887	26.611972358180825	22.52476705478203
46	22.400243015443547	29.153968048884295	26.98578198151639	21.460006954155773
47	21.58324846476561	27.059171125433213	28.04075686295373	23.31682354684745
48	22.441331435824164	25.399317694716363	30.166999490788594	21.992351378670882
49	22.416939639447925	24.91563811029367	30.038271607284	22.629150642974402
50	20.920371116313007	29.18080615005776	27.880393270809268	22.018429462819967
51	20.048085327111327	29.655365530187876	26.85102146288077	23.445527679820028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5542.0
1	6270.0
2	6998.0
3	27718.0
4	48438.0
5	34504.0
6	20570.0
7	21817.0
8	23064.0
9	24778.5
10	26493.0
11	27471.0
12	28449.0
13	28677.0
14	28905.0
15	28502.0
16	28099.0
17	27240.0
18	26381.0
19	25375.5
20	24370.0
21	24838.5
22	25307.0
23	26070.5
24	26834.0
25	28086.0
26	34723.5
27	40109.0
28	45152.0
29	50195.0
30	56891.0
31	63587.0
32	73175.0
33	82763.0
34	92960.0
35	103157.0
36	112161.0
37	121165.0
38	137910.5
39	154656.0
40	192242.5
41	229829.0
42	270829.5
43	311830.0
44	333611.0
45	355392.0
46	369107.5
47	382823.0
48	383796.0
49	384769.0
50	350170.5
51	315572.0
52	286832.5
53	258093.0
54	231470.5
55	204848.0
56	189485.5
57	174123.0
58	154631.0
59	135139.0
60	124820.0
61	114501.0
62	108614.5
63	102728.0
64	92017.0
65	81306.0
66	69817.0
67	58328.0
68	50675.0
69	43022.0
70	36804.5
71	30587.0
72	27673.0
73	24759.0
74	20288.5
75	12653.0
76	9488.0
77	7790.5
78	6093.0
79	5135.0
80	4177.0
81	2840.0
82	1503.0
83	1063.5
84	624.0
85	540.5
86	457.0
87	286.5
88	116.0
89	79.5
90	43.0
91	34.5
92	26.0
93	16.0
94	6.0
95	7.5
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4210432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.737459283921854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19629043131546	16.630673075409764
2	7.90426175071963	3.278286124500185
3	2.7057886340709056	1.6833354488983179
4	1.3545773337614617	1.1236196922320696
5	0.8499774902386296	0.8813186798036835
6	0.5903982587189911	0.7346015910890064
7	0.4472835194136789	0.6492866640547306
8	0.37271371841427786	0.6183308448140162
9	0.2991248143209982	0.5582779792013147
>10	3.0567303794716394	14.66834821754858
>50	1.0866757404547107	16.368354287663397
>100	1.1275435698943619	40.14440362019636
>500	0.006534109668822572	0.8951281828418884
>1k	0.0018668884768064417	0.845017071130107
>5k	1.166805298004026E-4	0.2130750602288384
>10k+	1.166805298004026E-4	0.7079434603878438
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29258	0.6948930656046695	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8806	0.2091471848969417	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	4394	0.10435983766036358	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06752276250988022	0.0
2	0.0	0.0	0.0	0.22123620569100746	0.0
3	0.0	0.0	0.0	0.3494415774913358	0.0
4	0.0	0.0	0.0	0.649220792545753	0.0
5	0.0	0.0	0.0	1.08366552410774	0.0
6	0.0	0.0	0.0	2.075915250501611	0.0
7	0.0	0.0	0.0	2.549643362011309	0.0
8	0.0	0.0	0.0	3.8384422311059767	0.0
9	0.0	0.0	0.0	4.483269175229525	0.0
10	0.0	0.0	0.0	5.401892252386453	0.0
11	0.0	0.0	0.0	6.005725778257433	0.0
12	0.0	0.0	0.0	6.53481637988691	0.0
13	0.0	0.0	0.0	6.80737748525567	0.0
14	0.0	2.3750532011917066E-5	0.0	6.933207803854806	0.0
15	0.0	2.3750532011917066E-5	0.0	7.04773286921627	0.0
16	0.0	2.3750532011917066E-5	0.0	7.251536184410531	0.0
17	0.0	2.3750532011917066E-5	0.0	7.466526000182404	0.0
18	0.0	2.3750532011917066E-5	0.0	7.80964993615857	0.0
19	0.0	2.3750532011917066E-5	0.0	7.954765686751383	0.0
20	0.0	2.3750532011917066E-5	0.0	8.111922957074238	0.0
21	0.0	2.3750532011917066E-5	0.0	8.300597183376908	0.0
22	0.0	2.3750532011917066E-5	0.0	8.491337705964614	0.0
23	0.0	2.3750532011917066E-5	0.0	8.712526410591597	0.0
24	0.0	2.3750532011917066E-5	0.0	8.896213025171765	0.0
25	0.0	2.3750532011917066E-5	0.0	9.058310406153097	0.0
26	0.0	2.3750532011917066E-5	0.0	9.210123806773272	0.0
27	0.0	2.3750532011917066E-5	0.0	9.365381034535174	0.0
28	0.0	2.3750532011917066E-5	0.0	9.51698068036724	0.0
29	0.0	2.3750532011917066E-5	0.0	9.685181948075638	0.0
30	0.0	2.3750532011917066E-5	0.0	9.93584506292941	0.0
31	2.3750532011917066E-5	2.3750532011917066E-5	0.0	10.12812937009789	0.0
32	2.3750532011917066E-5	2.3750532011917066E-5	0.0	10.307612140511948	0.0
33	2.3750532011917066E-5	2.3750532011917066E-5	0.0	10.487593672098255	0.0
34	2.3750532011917066E-5	2.3750532011917066E-5	0.0	10.666886438256217	0.0
35	2.3750532011917066E-5	2.3750532011917066E-5	0.0	10.870547250258406	0.0
36	2.3750532011917066E-5	2.3750532011917066E-5	0.0	11.056205158995562	0.0
37	4.750106402383413E-5	2.3750532011917066E-5	0.0	11.24834696297197	0.0
38	4.750106402383413E-5	2.3750532011917066E-5	0.0	11.452696540402505	0.0
39	4.750106402383413E-5	2.3750532011917066E-5	0.0	11.71019980847571	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAC	50	2.1827873E-11	45.0	19
CGTTTTT	19900	0.0	41.494976	1
GCGTAAT	45	1.9304935E-8	40.0	13
GCGTTAG	330	0.0	39.545452	1
GCGTAAG	275	0.0	39.272728	1
CGTTAGG	700	0.0	39.214287	2
TGATTCG	670	0.0	38.955223	15
TATAGCG	215	0.0	38.720932	1
ACGGGAT	780	0.0	38.365383	5
AGGGCGA	5315	0.0	38.184383	6
TCGTTAG	225	0.0	38.0	1
TCACGAC	300	0.0	37.500004	25
TACGGGA	935	0.0	37.299465	4
GGGCGAT	11140	0.0	36.618042	7
TAGGGCG	2065	0.0	36.610172	5
TAGGGAC	2335	0.0	36.231262	5
AGGGTAC	2110	0.0	36.042652	6
GGCGATA	2195	0.0	35.87699	8
GCGATAC	365	0.0	35.75342	9
CCGTAAG	145	0.0	35.689655	1
>>END_MODULE