FastQCFastQC Report
Thu 26 May 2016
SRR1546698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546698_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2777550
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474291.707584021889795No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35780.12881856312217602No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT28640.10311245522132814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT343400.043.958211
CGACGGT1200.041.25000428
TAGACGG3250.040.8461532
GTTTTTT393850.038.8644142
CGCGATC356.2514864E-638.57142635
TATCGAG1000.038.2500041
TGGGCGA18750.038.166
TAATGCG659.094947E-1238.076921
TAGGGCG11200.037.5669635
CAATGCG601.5643309E-1037.5000041
TAAGGGA29700.037.2727284
CGTAAGG5400.037.0833362
AGGGCGA29550.036.9289326
TTACGAG1100.036.818181
ATAGGGC16050.036.7289734
AAGGGCG12350.036.6194345
TAGGGAC21450.036.6083955
GGGCGAT58700.036.528967
TACGGGA8600.036.366284
AGGGTAC15000.036.36
Produced by FastQC (version 0.11.5)