##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546698_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2777550
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30154812694641	31.0	31.0	33.0	30.0	34.0
2	31.69859444474447	31.0	31.0	34.0	30.0	34.0
3	31.598247376284856	31.0	31.0	34.0	30.0	34.0
4	35.50262677539558	37.0	35.0	37.0	33.0	37.0
5	35.4685460207737	37.0	35.0	37.0	33.0	37.0
6	35.55540638332343	37.0	35.0	37.0	33.0	37.0
7	35.97611924177782	37.0	35.0	37.0	35.0	37.0
8	35.96814170762003	37.0	35.0	37.0	35.0	37.0
9	37.70485715828698	39.0	37.0	39.0	35.0	39.0
10	37.08003672301129	39.0	37.0	39.0	33.0	39.0
11	36.86059944915483	39.0	37.0	39.0	32.0	39.0
12	36.49708772119314	39.0	35.0	39.0	32.0	39.0
13	36.41283001206099	39.0	35.0	39.0	32.0	39.0
14	37.53395186405285	40.0	36.0	41.0	32.0	41.0
15	37.68740472718763	40.0	36.0	41.0	33.0	41.0
16	37.70205000810066	40.0	36.0	41.0	33.0	41.0
17	37.621779985958845	40.0	35.0	41.0	32.0	41.0
18	37.38219798023438	39.0	36.0	41.0	32.0	41.0
19	37.17678637648287	38.0	36.0	41.0	32.0	41.0
20	36.90466310237439	38.0	35.0	41.0	32.0	41.0
21	36.810498460873795	38.0	35.0	41.0	32.0	41.0
22	36.72373710644273	38.0	35.0	41.0	32.0	41.0
23	36.640445716548754	38.0	35.0	40.0	31.0	41.0
24	36.50587784198304	38.0	35.0	40.0	31.0	41.0
25	36.408321362351714	38.0	35.0	40.0	31.0	41.0
26	36.27751939659052	38.0	35.0	40.0	31.0	41.0
27	36.173591834530434	38.0	35.0	40.0	31.0	41.0
28	36.066279274900545	37.0	35.0	40.0	30.0	41.0
29	36.01532573671041	38.0	35.0	40.0	30.0	41.0
30	35.91152202480603	37.0	35.0	40.0	30.0	41.0
31	35.70400784864359	38.0	35.0	40.0	30.0	41.0
32	35.342455761372435	38.0	34.0	40.0	27.0	41.0
33	34.88355601159295	38.0	34.0	40.0	24.0	41.0
34	34.444683984086694	38.0	34.0	40.0	21.0	41.0
35	34.10603049450055	38.0	33.0	40.0	18.0	41.0
36	33.903817032996706	37.0	33.0	40.0	18.0	41.0
37	33.81542582491765	37.0	33.0	40.0	17.0	41.0
38	33.724297672409136	37.0	33.0	40.0	16.0	41.0
39	33.61991683318032	37.0	33.0	40.0	15.0	41.0
40	33.504610178034596	37.0	33.0	40.0	15.0	41.0
41	33.437508235675324	37.0	33.0	40.0	15.0	41.0
42	33.35297906428327	37.0	33.0	40.0	15.0	41.0
43	33.22470558585804	37.0	33.0	40.0	14.0	41.0
44	33.075765332757285	36.0	33.0	40.0	12.0	41.0
45	32.96807006174506	36.0	32.0	40.0	12.0	41.0
46	32.9329538622167	36.0	32.0	40.0	12.0	41.0
47	32.900072005904484	36.0	32.0	40.0	12.0	41.0
48	32.78046011772965	36.0	32.0	40.0	10.0	41.0
49	32.69214559593887	36.0	32.0	40.0	10.0	41.0
50	32.597715252650715	35.0	32.0	40.0	10.0	41.0
51	32.24154740688736	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	6.0
14	28.0
15	90.0
16	261.0
17	755.0
18	1795.0
19	3610.0
20	6326.0
21	10114.0
22	15102.0
23	23388.0
24	36789.0
25	59261.0
26	81294.0
27	82361.0
28	73251.0
29	68793.0
30	72751.0
31	82107.0
32	97946.0
33	122024.0
34	189305.0
35	290901.0
36	207608.0
37	250146.0
38	356482.0
39	644666.0
40	382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.865168943853398	23.308635308095262	26.428075102158378	22.398120645892963
2	36.80679015679286	24.37165847599503	26.353368976256053	12.46818239095606
3	25.521304746989255	22.938236935428705	38.67253514788212	12.867923169699916
4	21.701103490486222	26.595452827131826	38.010584867959174	13.692858814422781
5	20.094759770301167	30.160069125668304	37.01809148350165	12.727079620528883
6	18.122410037623084	38.27999495958669	34.09911612752246	9.498478875267773
7	80.04878400028802	3.5657323900559845	14.951666036615002	1.4338175730409894
8	80.68283199222336	2.8060700977480155	14.526795197206171	1.9843027128224513
9	75.55125920325466	4.878076002232183	16.77643966805278	2.7942251264603697
10	36.6442008244676	25.268600025202065	24.75469388489856	13.332505265431765
11	29.990927256034993	24.582635776133642	30.70216557757736	14.724271390254001
12	26.590520422674658	21.87078540440316	34.384367518136486	17.15432665478569
13	19.84259509279761	29.114435383701466	35.13549711076308	15.907472412737844
14	17.287105542654498	31.617360623571134	35.333549351046784	15.761984482727584
15	16.616766574859138	24.609241957840545	43.575237169447895	15.198754297852425
16	18.114453385177583	22.69111267123904	42.731363971845695	16.46306997173768
17	18.260949397850624	23.489226116541555	35.38240535723929	22.867419128368528
18	20.07042177458552	24.430307285197387	37.76400064805314	17.735270292163957
19	23.38902990045184	26.064157260895392	33.14500189015499	17.401810948497776
20	24.29007578621447	25.56717250814567	32.40355709168152	17.739194613958347
21	21.226800597649007	26.918759338265737	34.58558801821749	17.26885204586776
22	20.89078504437364	24.814386779715935	33.48234955266332	20.812478623247106
23	18.241111771165237	28.26584579935555	32.9036741012763	20.589368328202912
24	18.4861838670771	25.714568594624758	38.398984716746774	17.40026282155137
25	18.32341451999064	26.74295692246764	36.47869525301075	18.45493330453097
26	17.761516444348434	30.06372522546849	33.787402567010496	18.38735576317258
27	18.109232957102485	29.44541052366294	34.14491188277439	18.30044463646019
28	17.618152688520457	28.056596640924557	37.650951378013	16.67429929254199
29	17.975409983618658	25.809940415114042	37.18035678925672	19.034292812010584
30	18.85589818365106	27.718168889848965	35.60555165523573	17.820381271264242
31	22.173210203236664	27.128584543932604	32.3292830012061	18.368922251624635
32	24.07650627351443	26.400424834836457	32.1368832244244	17.386185667224712
33	22.019441594210726	27.82977804179943	30.740364709906213	19.410415654083636
34	19.806736152364493	28.142067649547265	31.845403323072492	20.205792875015753
35	19.342046047775916	27.874565714388577	31.572932980504405	21.210455257331102
36	22.766286835520514	27.84709546182787	29.48623787150546	19.900379831146154
37	19.841551007182588	30.089791362891756	30.544796673327212	19.52386095659844
38	20.07344602257385	30.485787834602434	28.53615596478911	20.9046101780346
39	19.353206962970965	30.715558675811415	29.158070961818872	20.77316339939875
40	21.408831524184983	28.194271930298285	29.21747583301831	21.179420712498427
41	18.355997191769724	28.110889092905616	29.896671527065223	23.636442188259437
42	20.763622617054597	28.914186963330994	27.974473906860364	22.347716512754047
43	20.611834170401973	28.44182822991485	29.38870587388166	21.557631725801514
44	20.4689024500009	30.10797285377401	29.17585642022646	20.247268275998632
45	18.807258195172004	31.965617180608806	28.13835934546633	21.08876527875286
46	20.99710176234451	30.478479235297296	28.451693038829184	20.07272596352901
47	20.685892243163938	29.245450126910406	28.72362333711364	21.34503429281201
48	21.006678547640906	28.202444600457238	29.688718474914943	21.102158376986914
49	20.478911270724197	27.32292848013537	30.287915609079946	21.910244640060487
50	19.451603031448577	30.060772983384638	29.664668502817232	20.822955482349553
51	18.716566758474194	30.68693632877896	28.521322748465373	22.07517416428147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3249.0
1	3477.0
2	3705.0
3	37138.0
4	70571.0
5	49578.5
6	28586.0
7	29770.5
8	30955.0
9	33539.5
10	36124.0
11	38215.0
12	40306.0
13	40579.5
14	40853.0
15	40334.0
16	39815.0
17	37650.5
18	35486.0
19	33832.0
20	32178.0
21	30880.0
22	29582.0
23	28630.0
24	27678.0
25	29002.0
26	31875.0
27	33424.0
28	37050.0
29	40676.0
30	43049.5
31	45423.0
32	51584.0
33	57745.0
34	65277.0
35	72809.0
36	79029.5
37	85250.0
38	96478.5
39	107707.0
40	128250.0
41	148793.0
42	169871.0
43	190949.0
44	193708.0
45	196467.0
46	200679.5
47	204892.0
48	214188.5
49	223485.0
50	220212.5
51	216940.0
52	198798.0
53	180656.0
54	157542.5
55	134429.0
56	119376.0
57	104323.0
58	92900.5
59	81478.0
60	72099.5
61	62721.0
62	54126.5
63	45532.0
64	39215.0
65	32898.0
66	27926.0
67	22954.0
68	18837.0
69	14720.0
70	11858.0
71	8996.0
72	7735.0
73	6474.0
74	4950.5
75	2830.0
76	2233.0
77	1810.5
78	1388.0
79	979.5
80	571.0
81	492.5
82	414.0
83	316.0
84	218.0
85	143.5
86	69.0
87	58.0
88	47.0
89	29.0
90	11.0
91	9.0
92	7.0
93	7.0
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2777550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.553377346059992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93992679021508	22.540015173113616
2	6.823026290160341	3.8964088861007204
3	2.960387396382105	2.53587175258255
4	1.7223219163411754	1.9671243035471502
5	1.2131813540165401	1.7320212495219134
6	0.8993093101272748	1.5406990849733384
7	0.7102127065193456	1.419527998364941
8	0.5340779542569549	1.2199783488088483
9	0.45468466927851053	1.1684504641840023
>10	4.461475603682864	28.010275553264073
>50	0.982413516211428	19.761815120168713
>100	0.2955205876518568	11.330827772948677
>500	0.0017950619330838772	0.34732639352279926
>1k	0.0015386245140718948	0.7932455959163583
>5k	0.0	0.0
>10k+	1.2821870950599124E-4	1.7364123029822753
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47429	1.707584021889795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3578	0.12881856312217602	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT	2864	0.10311245522132814	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.20059044841677E-5	0.0	0.0	0.05119619808824324	0.0
2	7.20059044841677E-5	0.0	0.0	0.14865618980756423	0.0
3	7.20059044841677E-5	0.0	0.0	0.27815880902233986	0.0
4	7.20059044841677E-5	0.0	0.0	0.4422962682940001	0.0
5	7.20059044841677E-5	0.0	0.0	0.69730517902468	0.0
6	7.20059044841677E-5	0.0	0.0	1.3888498856906266	0.0
7	7.20059044841677E-5	0.0	0.0	1.7825061655055714	0.0
8	7.20059044841677E-5	0.0	0.0	2.66897085561016	0.0
9	7.20059044841677E-5	0.0	0.0	3.198358265377761	0.0
10	1.440118089683354E-4	0.0	0.0	3.8476715090637432	0.0
11	1.440118089683354E-4	0.0	0.0	4.240427715072636	0.0
12	1.440118089683354E-4	0.0	0.0	4.59883710464258	0.0
13	1.440118089683354E-4	0.0	0.0	4.834872459541683	0.0
14	1.440118089683354E-4	0.0	0.0	4.963511007902648	0.0
15	1.440118089683354E-4	0.0	0.0	5.0572266925887925	0.0
16	1.440118089683354E-4	0.0	0.0	5.190689636550197	0.0
17	1.440118089683354E-4	0.0	0.0	5.336141563608216	0.0
18	1.440118089683354E-4	0.0	0.0	5.570412773847456	0.0
19	1.440118089683354E-4	0.0	0.0	5.68486615902504	0.0
20	1.440118089683354E-4	0.0	0.0	5.811992583391838	0.0
21	1.440118089683354E-4	0.0	0.0	5.951215999711977	0.0
22	1.440118089683354E-4	0.0	0.0	6.1038685172184115	0.0
23	1.440118089683354E-4	0.0	0.0	6.277330741120772	0.0
24	1.440118089683354E-4	0.0	0.0	6.420154452665119	0.0
25	1.440118089683354E-4	0.0	0.0	6.549441054166442	0.0
26	1.440118089683354E-4	0.0	0.0	6.674011268924052	0.0
27	1.440118089683354E-4	0.0	0.0	6.808086263073572	0.0
28	1.440118089683354E-4	0.0	0.0	6.946085579017479	0.0
29	1.8001476121041926E-4	0.0	0.0	7.094237727493654	0.0
30	1.8001476121041926E-4	0.0	0.0	7.3245486129862645	0.0
31	1.8001476121041926E-4	0.0	0.0	7.489838166729672	0.0
32	1.8001476121041926E-4	0.0	0.0	7.644506849561664	0.0
33	1.8001476121041926E-4	0.0	0.0	7.802955842379075	0.0
34	1.8001476121041926E-4	0.0	0.0	7.960000720059045	0.0
35	1.8001476121041926E-4	0.0	0.0	8.132166837680689	0.0
36	1.8001476121041926E-4	0.0	0.0	8.298140447516696	0.0
37	1.8001476121041926E-4	0.0	0.0	8.469010458857626	0.0
38	1.8001476121041926E-4	0.0	0.0	8.645280913034869	0.0
39	1.8001476121041926E-4	0.0	0.0	8.8393368256197	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34340	0.0	43.95821	1
CGACGGT	120	0.0	41.250004	28
TAGACGG	325	0.0	40.846153	2
GTTTTTT	39385	0.0	38.864414	2
CGCGATC	35	6.2514864E-6	38.571426	35
TATCGAG	100	0.0	38.250004	1
TGGGCGA	1875	0.0	38.16	6
TAATGCG	65	9.094947E-12	38.07692	1
TAGGGCG	1120	0.0	37.566963	5
CAATGCG	60	1.5643309E-10	37.500004	1
TAAGGGA	2970	0.0	37.272728	4
CGTAAGG	540	0.0	37.083336	2
AGGGCGA	2955	0.0	36.928932	6
TTACGAG	110	0.0	36.81818	1
ATAGGGC	1605	0.0	36.728973	4
AAGGGCG	1235	0.0	36.619434	5
TAGGGAC	2145	0.0	36.608395	5
GGGCGAT	5870	0.0	36.52896	7
TACGGGA	860	0.0	36.36628	4
AGGGTAC	1500	0.0	36.3	6
>>END_MODULE