FastQCFastQC Report
Thu 26 May 2016
SRR1546697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546697_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2818076
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232900.8264503867177465No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37890.13445343560642084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT132150.040.6923941
TAACGCG1000.040.51
CGGTCTA2000.040.531
TCACGAC1900.040.2631625
TAATACG403.4606092E-739.3751
TAGGGAT24300.038.3333365
CGACGGT2000.038.2528
CACGACG2000.038.2526
TACGGGA7300.038.2191774
ATCGGGT659.094947E-1238.076924
ACGGGAT7000.037.6071435
ACGGGTA3250.037.3846175
GCGATCG2100.036.428579
TAGTAGG14300.036.3461532
AGTACGG1800.036.2500042
GACCGAT19950.036.0902259
GGGCGAT64300.036.0419927
GCGTATT250.00210762736.09
AGGGCGA32050.035.873646
TAAGGGA26100.035.8620724
Produced by FastQC (version 0.11.5)