FastQCFastQC Report
Thu 26 May 2016
SRR1546696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546696_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2301376
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142210.617934661698045No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56730.24650469979699102No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC207.0347614E-445.00000420
ACGTAAC207.0347614E-445.00000418
CGTTTTT86550.041.022531
TACGCGT556.184564E-1140.90909223
TAAGTCG403.4602454E-739.3750041
CAATACG403.4602454E-739.3750041
ACGGGTC1200.039.3749965
AATACGG1950.038.0769272
CGTTAGT301.1402776E-437.49999626
CCATACG301.1402776E-437.4999961
TAGGGCG10100.037.425745
ATAGGGC12550.037.1115534
GGGCGAT51100.036.98637
CATATGC14300.036.9755333
AGGGTAC10050.036.94036
TAGTACG1100.036.818181
GCGCGAC5750.036.7826089
TATACGG1350.036.6666642
CGAGGGA12550.036.5737044
CTTAACG800.036.5625041
Produced by FastQC (version 0.11.5)