##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546696_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2301376
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348441975583302	31.0	31.0	33.0	30.0	34.0
2	31.74258139478295	31.0	31.0	34.0	30.0	34.0
3	31.85572587877861	31.0	31.0	34.0	30.0	34.0
4	35.624080550070914	37.0	35.0	37.0	33.0	37.0
5	35.53535754261798	37.0	35.0	37.0	33.0	37.0
6	35.59359009566451	37.0	35.0	37.0	33.0	37.0
7	35.968985511276735	37.0	35.0	37.0	35.0	37.0
8	35.94510849161545	37.0	35.0	37.0	35.0	37.0
9	37.667999927000196	39.0	37.0	39.0	35.0	39.0
10	37.095739679218	39.0	37.0	39.0	33.0	39.0
11	36.86695090241664	39.0	37.0	39.0	32.0	39.0
12	36.29182541227509	38.0	35.0	39.0	32.0	39.0
13	36.09079872215579	38.0	35.0	39.0	32.0	39.0
14	37.13286920520593	39.0	35.0	41.0	32.0	41.0
15	37.33850270446898	39.0	35.0	41.0	32.0	41.0
16	37.41016113837982	39.0	35.0	41.0	32.0	41.0
17	37.31756001626853	39.0	35.0	41.0	32.0	41.0
18	37.22027821616285	39.0	35.0	41.0	32.0	41.0
19	37.1578599064212	39.0	35.0	41.0	32.0	41.0
20	37.027853336438724	39.0	35.0	41.0	32.0	41.0
21	36.90707342042326	38.0	35.0	41.0	32.0	41.0
22	36.80100079256931	38.0	35.0	41.0	31.0	41.0
23	36.74319233362997	38.0	35.0	41.0	31.0	41.0
24	36.61485041992269	38.0	35.0	41.0	31.0	41.0
25	36.51725706707639	38.0	35.0	40.0	31.0	41.0
26	36.38022296226258	38.0	35.0	40.0	31.0	41.0
27	36.268974735114995	38.0	35.0	40.0	30.0	41.0
28	36.19142330501404	38.0	35.0	40.0	30.0	41.0
29	36.21915932033705	38.0	35.0	40.0	30.0	41.0
30	36.187943213103814	38.0	35.0	40.0	30.0	41.0
31	36.10453485219278	38.0	35.0	40.0	30.0	41.0
32	35.99516245932868	38.0	35.0	40.0	30.0	41.0
33	35.83609066923441	38.0	35.0	40.0	30.0	41.0
34	35.708271051753385	38.0	35.0	40.0	29.0	41.0
35	35.589391737812505	38.0	35.0	40.0	29.0	41.0
36	35.470281257821405	38.0	35.0	40.0	27.0	41.0
37	35.40073112781223	38.0	34.0	40.0	27.0	41.0
38	35.32832444589672	38.0	34.0	40.0	27.0	41.0
39	35.25224083331016	38.0	34.0	40.0	27.0	41.0
40	35.084177031480294	37.0	34.0	40.0	26.0	41.0
41	35.03105185767124	37.0	34.0	40.0	26.0	41.0
42	34.96412972065408	37.0	34.0	40.0	26.0	41.0
43	34.822225920492784	37.0	34.0	40.0	25.0	41.0
44	34.674734159042245	37.0	34.0	40.0	25.0	41.0
45	34.54974371854056	36.0	34.0	40.0	24.0	41.0
46	34.506458744681446	36.0	34.0	40.0	24.0	41.0
47	34.456956186212075	36.0	34.0	40.0	24.0	41.0
48	34.368949272087654	36.0	34.0	40.0	24.0	41.0
49	34.3163476980728	36.0	34.0	40.0	24.0	41.0
50	34.20000121666342	35.0	33.0	40.0	24.0	41.0
51	33.83119142634667	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	9.0
12	6.0
13	17.0
14	34.0
15	83.0
16	190.0
17	496.0
18	1073.0
19	2150.0
20	3620.0
21	5858.0
22	8777.0
23	12988.0
24	18260.0
25	26533.0
26	34629.0
27	40492.0
28	44695.0
29	49332.0
30	57527.0
31	69209.0
32	84993.0
33	109613.0
34	182889.0
35	289412.0
36	170958.0
37	214154.0
38	315268.0
39	557749.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.60574195611669	24.03231805667566	26.068882268694903	14.29305771851275
2	32.039658013292915	25.53424559915459	27.70633742595734	14.71975896159515
3	28.302806668706026	25.904632706693736	31.232662546233207	14.559898078367029
4	25.42774409744431	28.681623515670623	30.51913290135988	15.371499485525181
5	23.196644094663366	32.66211171055925	29.337665813843543	14.80357838093384
6	21.394461400483884	40.53518416808031	27.233489877360327	10.836864554075474
7	86.38318988292222	4.299992700019466	7.5064222447787765	1.8103951722795406
8	86.64386002113518	3.618052851859062	7.186135598876499	2.5519515281292584
9	80.9496579437693	5.684903292638839	9.807828012458634	3.5576107511332355
10	43.314347590311186	26.024560958313636	16.477403084067966	14.183688367307212
11	37.252756611696654	24.56234878611752	23.47895346088601	14.705941141299814
12	32.848130857365334	23.12125441475013	26.643103951722797	17.38751077616174
13	22.407507508551404	33.11479740815929	27.663667301649102	16.8140277816402
14	18.555942184154176	34.88304388331155	29.33810033649434	17.222913596039934
15	17.656132678884283	24.10240656024917	41.22629244417253	17.015168316694012
16	20.291947078617316	21.794482952807364	39.09752252565421	18.816047442921104
17	19.578417433743986	22.30052803192525	29.88877089184905	28.232283642481715
18	23.792765719291413	22.771637489919076	33.3505693984816	20.085027392307904
19	27.249871381295364	24.934300175199535	27.636596540504467	20.179231903000638
20	28.945987096415358	24.176623029005256	26.181988514697295	20.695401359882087
21	24.32822798186824	27.18982035095526	28.96254240941072	19.519409257765787
22	23.62230248338385	23.980870574821324	27.195251884090215	25.201575057704606
23	21.418447050807863	28.507162671375735	26.285448357851998	23.788941919964405
24	21.325937178453238	24.69348772212798	34.905812870213296	19.074762229205483
25	20.880421104591342	25.594861508940735	32.17005826079702	21.354659125670903
26	19.974919352596014	29.860048944631384	28.637215300759202	21.527816402013407
27	20.003945465669233	29.61671626018521	29.364475861397704	21.01486241274785
28	18.442749033621624	28.3220994744014	33.84570795906449	19.389443532912484
29	19.504244417252984	25.816859131232796	33.33253670847354	21.346359743040686
30	21.239858241330403	27.43828040268083	31.77238313078784	19.54947822520092
31	26.335288105898385	27.05151179120665	26.71545197308045	19.89774812981451
32	27.177957882588505	26.42627714897522	27.22101907728246	19.174745891153812
33	25.412144734280705	27.99433903890542	25.82246447342807	20.771051753385798
34	21.191148252176088	27.633945952334603	28.548442323201424	22.626463472287885
35	20.196047929586474	28.626699852609917	29.7486373369671	21.42861488083651
36	25.877953015934814	27.613827553602714	26.545423259823686	19.962796170638782
37	20.9495536583331	31.420419783642483	27.788809825078566	19.841216732945853
38	21.419750618760254	31.9676576100559	25.11727766345004	21.495314107733808
39	21.658607719903223	30.248772908034148	26.34493450874607	21.747684863316554
40	23.671490447454044	27.04642787619233	27.220714911426903	22.06136676492672
41	19.02713854667816	26.167605814955923	28.53944770432993	26.265807934035983
42	23.122036555521568	26.533430434661696	26.521741775355267	23.82279123446147
43	22.667873480908813	25.7058820462193	27.619606704858313	24.006637768013572
44	21.083864609694373	28.69509371784532	28.086892363525124	22.134149308935175
45	19.563947829472454	31.868934063794878	26.419237882032316	22.14788022470035
46	22.500842973942543	29.593469298367587	26.79436128646514	21.11132644122473
47	21.74824974276259	28.628090325092465	27.53213729525293	22.091522636892016
48	22.604650435217888	26.851240301454432	29.430479852053725	21.11362941127395
49	22.52682742846019	25.116799688534165	30.00183368558636	22.35453919741928
50	20.640347340026143	29.90480477766345	28.23606398954365	21.218783892766762
51	20.33648565032398	30.150918407074723	26.190070636002115	23.322525306599182
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2724.0
1	3433.0
2	4142.0
3	14064.0
4	23986.0
5	16867.5
6	9749.0
7	10078.5
8	10408.0
9	11216.0
10	12024.0
11	12269.5
12	12515.0
13	12705.5
14	12896.0
15	12259.0
16	11622.0
17	12066.5
18	12511.0
19	11910.0
20	11309.0
21	10993.0
22	10677.0
23	12082.5
24	13488.0
25	14808.5
26	20210.0
27	24291.0
28	25718.0
29	27145.0
30	31298.5
31	35452.0
32	40973.0
33	46494.0
34	54464.5
35	62435.0
36	65290.5
37	68146.0
38	78764.0
39	89382.0
40	109123.0
41	128864.0
42	153354.5
43	177845.0
44	190144.5
45	202444.0
46	206071.0
47	209698.0
48	196104.5
49	182511.0
50	174436.5
51	166362.0
52	155265.5
53	144169.0
54	130126.5
55	116084.0
56	106416.5
57	96749.0
58	89180.0
59	81611.0
60	75365.0
61	69119.0
62	62634.0
63	56149.0
64	51442.5
65	46736.0
66	40549.0
67	34362.0
68	28274.5
69	22187.0
70	19329.0
71	16471.0
72	14364.5
73	12258.0
74	10646.5
75	7261.5
76	5488.0
77	4290.5
78	3093.0
79	2215.0
80	1337.0
81	1011.0
82	685.0
83	490.5
84	296.0
85	241.0
86	186.0
87	123.5
88	61.0
89	45.5
90	30.0
91	20.0
92	10.0
93	9.0
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2301376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.578642888761625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.26484110063662	17.320063426338344
2	6.74057284884968	2.909048287420197
3	2.3789163624117857	1.5400135994014728
4	1.2285184506064866	1.0603904371156845
5	0.8082500358701765	0.8720469444435658
6	0.5926280128907365	0.7672864953627375
7	0.4788814389944126	0.7233528090681701
8	0.3899490246445623	0.6731656598100735
9	0.3417889754530494	0.6637808020155361
>10	4.397225188584493	23.02610841956561
>50	1.5449438150745824	23.59918114975143
>100	0.8290069295204245	25.313569386003753
>500	0.0036636680152394388	0.5272661611582101
>1k	4.070742239154932E-4	0.13097106976874492
>5k	2.035371119577466E-4	0.24916126049567064
>10k+	2.035371119577466E-4	0.624594092280792
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14221	0.617934661698045	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5673	0.24650469979699102	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3452265079674076E-5	0.0	0.0	0.03550050057009372	0.0
2	4.3452265079674076E-5	0.0	0.0	0.14009010261686922	0.0
3	4.3452265079674076E-5	0.0	0.0	0.21508871214438666	0.0
4	4.3452265079674076E-5	0.0	0.0	0.35565678967713227	0.0
5	4.3452265079674076E-5	0.0	0.0	0.5964257904836063	0.0
6	4.3452265079674076E-5	0.0	0.0	1.077355460385439	0.0
7	4.3452265079674076E-5	0.0	0.0	1.3187762451681082	0.0
8	4.3452265079674076E-5	0.0	0.0	2.051251077616174	0.0
9	4.3452265079674076E-5	0.0	0.0	2.4106013098250787	0.0
10	4.3452265079674076E-5	0.0	0.0	2.9325499179621235	0.0
11	9.124975666731555E-4	0.0	0.0	3.3005906031869627	0.0
12	9.124975666731555E-4	0.0	0.0	3.61566297727968	0.0
13	9.124975666731555E-4	0.0	0.0	3.771308990795072	0.0
14	9.124975666731555E-4	0.0	0.0	3.8447433187797215	0.0
15	9.124975666731555E-4	0.0	0.0	3.9232181095136127	0.0
16	9.124975666731555E-4	0.0	0.0	4.072737353652771	0.0
17	9.124975666731555E-4	0.0	0.0	4.2263845629744985	0.0
18	9.124975666731555E-4	0.0	0.0	4.446470285603048	0.0
19	9.124975666731555E-4	0.0	0.0	4.551668219360939	0.0
20	9.124975666731555E-4	0.0	0.0	4.669945284907811	0.0
21	9.124975666731555E-4	0.0	0.0	4.8129466892850195	0.0
22	9.124975666731555E-4	0.0	0.0	4.9619445062432215	0.0
23	9.124975666731555E-4	0.0	0.0	5.132190480825384	0.0
24	9.124975666731555E-4	0.0	0.0	5.267891904669207	0.0
25	9.124975666731555E-4	0.0	0.0	5.383301120720821	0.0
26	9.559498317528296E-4	0.0	0.0	5.496363914458133	0.0
27	9.559498317528296E-4	0.0	0.0	5.61933382463361	0.0
28	9.559498317528296E-4	0.0	0.0	5.735568633721739	0.0
29	9.559498317528296E-4	0.0	0.0	5.866446856141717	0.0
30	9.559498317528296E-4	0.0	0.0	6.066631441363775	0.0
31	9.559498317528296E-4	0.0	0.0	6.215064378875942	0.0
32	9.559498317528296E-4	0.0	0.0	6.364974693400818	0.0
33	9.559498317528296E-4	0.0	0.0	6.512842751466948	0.0
34	9.559498317528296E-4	0.0	0.0	6.655713799048917	0.0
35	9.559498317528296E-4	0.0	0.0	6.823960969437415	0.0
36	9.559498317528296E-4	0.0	0.0	6.968874273478128	0.0
37	9.559498317528296E-4	0.0	0.0	7.126562543452265	0.0
38	9.559498317528296E-4	0.0	0.0	7.291941864345505	0.0
39	9.559498317528296E-4	0.0	0.0	7.490388358964376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAC	20	7.0347614E-4	45.000004	20
ACGTAAC	20	7.0347614E-4	45.000004	18
CGTTTTT	8655	0.0	41.02253	1
TACGCGT	55	6.184564E-11	40.909092	23
TAAGTCG	40	3.4602454E-7	39.375004	1
CAATACG	40	3.4602454E-7	39.375004	1
ACGGGTC	120	0.0	39.374996	5
AATACGG	195	0.0	38.076927	2
CGTTAGT	30	1.1402776E-4	37.499996	26
CCATACG	30	1.1402776E-4	37.499996	1
TAGGGCG	1010	0.0	37.42574	5
ATAGGGC	1255	0.0	37.111553	4
GGGCGAT	5110	0.0	36.9863	7
CATATGC	1430	0.0	36.97553	33
AGGGTAC	1005	0.0	36.9403	6
TAGTACG	110	0.0	36.81818	1
GCGCGAC	575	0.0	36.782608	9
TATACGG	135	0.0	36.666664	2
CGAGGGA	1255	0.0	36.573704	4
CTTAACG	80	0.0	36.562504	1
>>END_MODULE