FastQCFastQC Report
Thu 26 May 2016
SRR1546695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546695_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2293634
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156070.6804485807238644No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46680.20351982923169082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1950.041.538461
CGTTTTT87500.040.7828561
ATAGCGG4350.040.344832
CGTTGAT4450.039.438225
TCGATAG1100.038.8636361
CGTAAGG4000.038.81252
ACTACGG1450.038.7931062
TACGGGT2500.038.6999974
TTTACGG2050.038.4146352
TAGGGCG8850.037.6271175
CGTTATC301.1402761E-437.50000444
TACGACG301.1402761E-437.5000041
ACGTAGT301.1402761E-437.50000436
ATACCGG1200.037.5000042
TACGTAG2400.037.5000041
TCGTAAG1500.037.51
AGGGCGA23250.037.2580646
TAACGGG6100.037.25413
CGGTCTA1150.037.17391631
TAGGGAT25750.037.135925
Produced by FastQC (version 0.11.5)