##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546695_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2293634
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.387662111740582	31.0	31.0	33.0	30.0	34.0
2	31.802984259912435	31.0	31.0	34.0	30.0	34.0
3	31.913433006312253	31.0	31.0	34.0	30.0	34.0
4	35.674356065527455	37.0	35.0	37.0	33.0	37.0
5	35.594340247833784	37.0	35.0	37.0	33.0	37.0
6	35.66096944848219	37.0	35.0	37.0	33.0	37.0
7	36.00070804670667	37.0	35.0	37.0	35.0	37.0
8	35.98145868085318	37.0	35.0	37.0	35.0	37.0
9	37.72737673055073	39.0	38.0	39.0	35.0	39.0
10	37.175668393475156	39.0	37.0	39.0	33.0	39.0
11	36.92694518829072	39.0	37.0	39.0	33.0	39.0
12	36.41560118135675	38.0	35.0	39.0	32.0	39.0
13	36.23975708417298	39.0	35.0	39.0	32.0	39.0
14	37.32218959084143	39.0	35.0	41.0	32.0	41.0
15	37.50477146746168	39.0	35.0	41.0	33.0	41.0
16	37.57531323654951	39.0	35.0	41.0	33.0	41.0
17	37.486385796513304	39.0	35.0	41.0	33.0	41.0
18	37.394419074708516	39.0	35.0	41.0	32.0	41.0
19	37.321455820763035	39.0	35.0	41.0	32.0	41.0
20	37.18499507768022	39.0	35.0	41.0	32.0	41.0
21	37.07628941670728	39.0	35.0	41.0	32.0	41.0
22	36.984308743243254	39.0	35.0	41.0	32.0	41.0
23	36.914936297595865	38.0	35.0	41.0	32.0	41.0
24	36.79999293697251	38.0	35.0	41.0	31.0	41.0
25	36.67658309913438	38.0	35.0	41.0	31.0	41.0
26	36.54119358188795	38.0	35.0	40.0	31.0	41.0
27	36.45130391335322	38.0	35.0	40.0	31.0	41.0
28	36.373103119329414	38.0	35.0	40.0	31.0	41.0
29	36.40542824182062	38.0	35.0	40.0	31.0	41.0
30	36.3911487185837	38.0	35.0	40.0	31.0	41.0
31	36.32555586462356	38.0	35.0	40.0	31.0	41.0
32	36.20659268218033	38.0	35.0	40.0	30.0	41.0
33	36.06146229084501	38.0	35.0	41.0	30.0	41.0
34	35.93201705241551	38.0	35.0	41.0	30.0	41.0
35	35.834857261446246	38.0	35.0	41.0	29.0	41.0
36	35.7072091711232	38.0	35.0	41.0	29.0	41.0
37	35.6523364233352	38.0	35.0	40.0	29.0	41.0
38	35.57230926991839	38.0	35.0	40.0	29.0	41.0
39	35.478707152056515	38.0	35.0	40.0	28.0	41.0
40	35.32423307293143	38.0	34.0	40.0	27.0	41.0
41	35.28913026228248	38.0	34.0	40.0	27.0	41.0
42	35.21848559970771	38.0	34.0	40.0	27.0	41.0
43	35.107338398366956	37.0	34.0	40.0	27.0	41.0
44	34.96421573799481	37.0	34.0	40.0	26.0	41.0
45	34.83468286570569	37.0	34.0	40.0	26.0	41.0
46	34.807047680667445	37.0	34.0	40.0	26.0	41.0
47	34.77228668566999	37.0	34.0	40.0	26.0	41.0
48	34.694252003589064	36.0	34.0	40.0	26.0	41.0
49	34.617624695134445	36.0	34.0	40.0	25.0	41.0
50	34.51469676504621	36.0	34.0	40.0	25.0	41.0
51	34.15346607174467	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	3.0
12	6.0
13	7.0
14	14.0
15	56.0
16	157.0
17	374.0
18	896.0
19	1806.0
20	3170.0
21	5039.0
22	7710.0
23	11335.0
24	16678.0
25	23830.0
26	31716.0
27	37000.0
28	40371.0
29	45917.0
30	53810.0
31	66043.0
32	81847.0
33	105867.0
34	177301.0
35	284674.0
36	171710.0
37	213373.0
38	320475.0
39	592069.0
40	371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.85012604452149	23.688217038987037	27.957075976376355	14.504580940115119
2	31.225644544857634	26.276773016095856	28.97450072679425	13.52308171225226
3	28.52285935768305	25.610799281838343	31.713298634394153	14.153042726084458
4	24.651055922610148	28.712819918086318	31.547753477669062	15.088370681634473
5	23.039813675590786	32.47488483341283	30.21083573054812	14.274465760448265
6	20.59051269731788	41.53858026171569	26.855723275814714	11.01518376515172
7	87.35643960631906	4.141157656365401	6.976047617013002	1.5263551203025418
8	87.65095041318712	3.4104395034255686	6.781639965225489	2.156970118161834
9	82.4385233215064	5.5768269915775575	9.002787715912827	2.98186197100322
10	43.12008803496983	29.81207986976126	14.861176630621975	12.206655464646932
11	35.773580266075584	24.705685388340076	24.640853771787476	14.879880573796866
12	33.10685139826145	22.44865571403284	27.148751718888022	17.295741168817695
13	22.344410660114036	32.41742143689883	28.094892210352658	17.143275692634482
14	17.749431687880453	34.72550546425454	29.488139781673972	18.036923066191033
15	17.25388619108367	25.025396379718824	40.90360537034243	16.817112058855074
16	19.071133406637678	22.096201922364248	40.05704484673666	18.775619824261412
17	18.655417560081514	22.230966230880778	30.629908695110032	28.48370751392768
18	22.347898574925207	23.5077610464442	32.88118330997884	21.263157068651754
19	26.70434777300999	25.47163148087271	27.420939870964588	20.403080875152703
20	29.189356279162237	23.29198991643828	27.067047314436394	20.45160648996309
21	23.7546618161398	26.97396358791333	28.54714396455581	20.72423063139106
22	23.807372928723588	24.135847306065397	27.250424435633587	24.806355329577432
23	21.13349383554656	28.666605046838335	26.327434978728082	23.872466138887024
24	21.486558012307107	24.190781964341305	35.19092409686986	19.13173592648173
25	20.50152727069794	25.973150031783625	31.95413915210535	21.571183545413085
26	19.627325022213657	30.6266823739097	28.258780607542445	21.487211996334203
27	19.90086474127956	30.51323794467644	28.554119794178145	21.031777519865855
28	18.419983310327627	28.922356400367278	33.67773585497948	18.979924434325614
29	19.045148441294472	25.11329183296027	33.236383834561224	22.605175891184036
30	20.64379059605848	28.102216831456104	31.1630800729323	20.09091249955311
31	26.048053002353473	27.16863283331168	26.78888610824569	19.99442805608916
32	26.490407798279936	26.032967770795167	27.924681967567622	19.551942463357275
33	25.22808782918286	27.403892687325005	25.882943834979777	21.48507564851236
34	20.64174144610692	27.998276970083282	28.575134480915438	22.78484710289436
35	20.726454177083177	27.094514643574346	29.726059170730814	22.452972008611663
36	25.793348023267882	27.11971482808504	26.447201253556585	20.6397358950905
37	21.39273310388667	30.185155957750887	28.22887173803667	20.19323920032577
38	21.545503772615856	30.341327343420964	26.006634013970842	22.106534869992338
39	22.185884931946422	30.00696710983531	26.461370907476955	21.34577705074131
40	23.904162564733518	26.475976550748726	27.679437957407327	21.94042292711043
41	19.01724512280512	26.408180206606634	29.045654188942088	25.528920481646157
42	22.808477725740026	26.43634511870682	26.518398314639562	24.23677884091359
43	22.65814859737866	26.02686391987562	28.001154499802496	23.313832982943225
44	21.840363370965026	28.723196464649547	27.70476893872344	21.731671225661984
45	20.13102351988155	31.638875252110843	26.125310315420858	22.10479091258675
46	22.314676186348827	29.67003453907642	26.358782613093457	21.656506661481302
47	21.484465263420407	28.383299166301164	27.782287845401665	22.349947724876767
48	23.048359066878152	26.020106084928983	29.37090224508357	21.5606326031093
49	21.905979768350136	26.14157271822793	29.411231260087707	22.541216253334227
50	20.82293862054713	29.780078251368792	28.178078978599025	21.218904149485052
51	20.330663043885817	29.451167884675584	26.64104211918728	23.57712695225132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2977.0
1	3493.5
2	4010.0
3	14743.5
4	25477.0
5	17750.0
6	10023.0
7	10155.0
8	10287.0
9	10971.0
10	11655.0
11	11852.5
12	12050.0
13	12060.0
14	12070.0
15	12052.5
16	12035.0
17	11443.0
18	10851.0
19	11197.5
20	11544.0
21	11652.0
22	11760.0
23	11801.5
24	11843.0
25	13719.0
26	19072.5
27	22550.0
28	26435.5
29	30321.0
30	35318.5
31	40316.0
32	45978.5
33	51641.0
34	60559.0
35	69477.0
36	73019.0
37	76561.0
38	83530.0
39	90499.0
40	110079.0
41	129659.0
42	150602.0
43	171545.0
44	176713.5
45	181882.0
46	179396.5
47	176911.0
48	175745.0
49	174579.0
50	176458.5
51	178338.0
52	165505.0
53	152672.0
54	137787.5
55	122903.0
56	113940.0
57	104977.0
58	96436.0
59	87895.0
60	82847.0
61	77799.0
62	68052.0
63	58305.0
64	49700.0
65	41095.0
66	35867.0
67	30639.0
68	25456.5
69	20274.0
70	17066.5
71	13859.0
72	12320.5
73	10782.0
74	9131.0
75	5819.5
76	4159.0
77	3250.5
78	2342.0
79	1619.0
80	896.0
81	740.0
82	584.0
83	424.5
84	265.0
85	197.5
86	130.0
87	99.5
88	69.0
89	50.5
90	32.0
91	21.5
92	11.0
93	8.0
94	5.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2293634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.172098947471262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79500228183169	16.09632681542804
2	6.426735378844514	2.5928148394253148
3	2.2275489598147757	1.3480301408316104
4	1.2426787234759498	1.0026975267949654
5	0.789059035961762	0.7958488474408474
6	0.6222233113678177	0.7530930122600903
7	0.5020707627970361	0.708947477404193
8	0.40537797452264385	0.6541859690557004
9	0.34330399900428904	0.6232646013279384
>10	4.978764606382375	24.660427087527008
>50	1.8221047867964149	25.946325124405927
>100	0.8409809619254012	23.345695221504915
>500	0.0030573179923363046	0.36620261966617385
>1k	8.735194263818013E-4	0.41862426256317214
>5k	0.0	0.0
>10k+	2.1837985659545032E-4	0.6875164543642458
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15607	0.6804485807238644	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4668	0.20351982923169082	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.71978702792163E-5	0.0	0.0	0.03243760774386847	0.0
2	8.71978702792163E-5	0.0	0.0	0.11244165372504943	0.0
3	8.71978702792163E-5	0.0	0.0	0.17853763939669537	0.0
4	8.71978702792163E-5	0.0	0.0	0.30785208102077316	0.0
5	8.71978702792163E-5	0.0	0.0	0.5209200770480382	0.0
6	8.71978702792163E-5	0.0	0.0	0.9901754159556407	0.0
7	8.71978702792163E-5	0.0	0.0	1.2213369700658432	0.0
8	8.71978702792163E-5	0.0	0.0	1.8790705055819716	0.0
9	8.71978702792163E-5	0.0	0.0	2.2200141783737073	0.0
10	8.71978702792163E-5	0.0	0.0	2.720660750581828	0.0
11	8.71978702792163E-5	0.0	0.0	3.042508089782415	0.0
12	8.71978702792163E-5	0.0	0.0	3.3062816473770447	0.0
13	8.71978702792163E-5	0.0	0.0	3.4529920641218257	0.0
14	8.71978702792163E-5	0.0	0.0	3.5207884082639165	0.0
15	8.71978702792163E-5	0.0	0.0	3.581826917459368	0.0
16	8.71978702792163E-5	0.0	0.0	3.693876180768161	0.0
17	8.71978702792163E-5	0.0	0.0	3.811026519488288	0.0
18	8.71978702792163E-5	0.0	0.0	3.9797543984785717	0.0
19	8.71978702792163E-5	0.0	0.0	4.061371605059918	0.0
20	8.71978702792163E-5	0.0	0.0	4.155632502831751	0.0
21	8.71978702792163E-5	0.0	0.0	4.263539867302281	0.0
22	8.71978702792163E-5	0.0	0.0	4.382739355973969	0.0
23	8.71978702792163E-5	0.0	0.0	4.51619569643631	0.0
24	8.71978702792163E-5	0.0	0.0	4.624975039609633	0.0
25	8.71978702792163E-5	0.0	0.0	4.716881594883927	0.0
26	8.71978702792163E-5	0.0	0.0	4.81105529478548	0.0
27	8.71978702792163E-5	0.0	0.0	4.909370893525296	0.0
28	8.71978702792163E-5	0.0	0.0	5.009779241151814	0.0
29	8.71978702792163E-5	0.0	0.0	5.115506658865364	0.0
30	8.71978702792163E-5	0.0	0.0	5.287460859055979	0.0
31	8.71978702792163E-5	0.0	0.0	5.408055513652134	0.0
32	8.71978702792163E-5	0.0	0.0	5.526077831075054	0.0
33	1.3079680541882446E-4	0.0	0.0	5.644100148497973	0.0
34	1.3079680541882446E-4	0.0	0.0	5.762689252077707	0.0
35	1.3079680541882446E-4	0.0	0.0	5.893311661755973	0.0
36	1.3079680541882446E-4	0.0	0.0	6.018571402412068	0.0
37	1.3079680541882446E-4	0.0	0.0	6.143177159041068	0.0
38	1.3079680541882446E-4	0.0	0.0	6.275935916541174	0.0
39	1.3079680541882446E-4	0.0	0.0	6.40660192515458	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	195	0.0	41.53846	1
CGTTTTT	8750	0.0	40.782856	1
ATAGCGG	435	0.0	40.34483	2
CGTTGAT	445	0.0	39.4382	25
TCGATAG	110	0.0	38.863636	1
CGTAAGG	400	0.0	38.8125	2
ACTACGG	145	0.0	38.793106	2
TACGGGT	250	0.0	38.699997	4
TTTACGG	205	0.0	38.414635	2
TAGGGCG	885	0.0	37.627117	5
CGTTATC	30	1.1402761E-4	37.500004	44
TACGACG	30	1.1402761E-4	37.500004	1
ACGTAGT	30	1.1402761E-4	37.500004	36
ATACCGG	120	0.0	37.500004	2
TACGTAG	240	0.0	37.500004	1
TCGTAAG	150	0.0	37.5	1
AGGGCGA	2325	0.0	37.258064	6
TAACGGG	610	0.0	37.2541	3
CGGTCTA	115	0.0	37.173916	31
TAGGGAT	2575	0.0	37.13592	5
>>END_MODULE