FastQCFastQC Report
Thu 26 May 2016
SRR1546694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546694_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5685625
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523150.9201275145652412No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC70490.12397933384632295No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT354000.041.5932241
CGTAAGG7650.037.6470572
GGGCGAT157900.037.3765077
CACGACC23850.037.3584927
AGGGCGA81250.036.9969256
TGGGCGA46200.036.9155856
ACACGAC23850.036.6037726
AGGGTAC29300.036.3993156
TATAGCG2800.036.1607131
CGGTCTA4050.036.1111131
TACGGGA12600.036.071434
CGTTCGG14250.035.84210645
GGCGATA32500.035.792318
CGTTAGG8050.035.7763982
ATAGGGC31100.035.6672024
GCGAGAC24650.035.5983821
CGAGACA24650.035.5983822
TAGCATA34150.035.51244430
GCGATAT8250.035.4545489
GACACGA24650.035.32454725
Produced by FastQC (version 0.11.5)