##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546694_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5685625
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.358623018577553	31.0	31.0	33.0	30.0	34.0
2	31.76581835770034	31.0	31.0	34.0	30.0	34.0
3	31.81184073870507	31.0	31.0	34.0	30.0	34.0
4	35.61860762888865	37.0	35.0	37.0	33.0	37.0
5	35.55224799384413	37.0	35.0	37.0	33.0	37.0
6	35.61720532043531	37.0	35.0	37.0	33.0	37.0
7	35.98672916346048	37.0	35.0	37.0	35.0	37.0
8	35.96175934923601	37.0	35.0	37.0	35.0	37.0
9	37.66554688358799	39.0	37.0	39.0	35.0	39.0
10	37.10301347697043	39.0	37.0	39.0	33.0	39.0
11	36.91484665274266	39.0	37.0	39.0	33.0	39.0
12	36.5838084643289	39.0	35.0	39.0	32.0	39.0
13	36.52156816532923	39.0	35.0	39.0	32.0	39.0
14	37.61302073210949	40.0	36.0	41.0	32.0	41.0
15	37.72261659887875	40.0	36.0	41.0	33.0	41.0
16	37.739977047378254	40.0	36.0	41.0	33.0	41.0
17	37.65539830713422	40.0	36.0	41.0	33.0	41.0
18	37.50849589974717	39.0	36.0	41.0	32.0	41.0
19	37.411588523689126	39.0	36.0	41.0	32.0	41.0
20	37.24485680993734	39.0	35.0	41.0	32.0	41.0
21	37.148925755743655	39.0	35.0	41.0	32.0	41.0
22	37.037678179619654	39.0	35.0	41.0	32.0	41.0
23	36.93252298559965	39.0	35.0	41.0	31.0	41.0
24	36.80813261514785	38.0	35.0	41.0	31.0	41.0
25	36.695822183137295	38.0	35.0	41.0	31.0	41.0
26	36.569858195009346	38.0	35.0	40.0	31.0	41.0
27	36.46169013960646	38.0	35.0	40.0	31.0	41.0
28	36.352236561503794	38.0	35.0	40.0	30.0	41.0
29	36.345255095086294	38.0	35.0	40.0	30.0	41.0
30	36.281292206221835	38.0	35.0	40.0	30.0	41.0
31	36.2002917885017	38.0	35.0	40.0	30.0	41.0
32	36.04915167637683	38.0	35.0	40.0	30.0	41.0
33	35.84324924700451	38.0	35.0	41.0	29.0	41.0
34	35.61012307354073	38.0	35.0	41.0	27.0	41.0
35	35.42479322853688	38.0	35.0	41.0	26.0	41.0
36	35.300346927558536	38.0	35.0	40.0	25.0	41.0
37	35.24823726503243	38.0	34.0	40.0	25.0	41.0
38	35.15104777399142	38.0	34.0	40.0	25.0	41.0
39	35.04459421787402	38.0	34.0	40.0	24.0	41.0
40	34.918651159722984	38.0	34.0	40.0	24.0	41.0
41	34.855716653841924	38.0	34.0	40.0	24.0	41.0
42	34.78231153127405	38.0	34.0	40.0	23.0	41.0
43	34.660125316038254	37.0	34.0	40.0	23.0	41.0
44	34.49061501593932	37.0	34.0	40.0	23.0	41.0
45	34.38690909090909	37.0	34.0	40.0	23.0	41.0
46	34.34562128174123	37.0	33.0	40.0	23.0	41.0
47	34.29808086182258	37.0	33.0	40.0	23.0	41.0
48	34.184071408156534	36.0	33.0	40.0	23.0	41.0
49	34.09266527426624	36.0	33.0	40.0	23.0	41.0
50	34.01197933384632	36.0	33.0	40.0	23.0	41.0
51	33.65078773221941	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	11.0
12	13.0
13	32.0
14	63.0
15	144.0
16	394.0
17	1107.0
18	2507.0
19	5056.0
20	8649.0
21	14253.0
22	21621.0
23	32193.0
24	48401.0
25	73028.0
26	100383.0
27	113151.0
28	116261.0
29	123069.0
30	139172.0
31	165463.0
32	202527.0
33	256776.0
34	398238.0
35	581618.0
36	459358.0
37	576370.0
38	824083.0
39	1420808.0
40	863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.95878201604925	22.91790260525448	26.698489611959985	16.424825766736287
2	35.92839837309003	24.18091238869957	26.819232714081565	13.071456524128832
3	29.006802242497525	24.954952182038035	32.11249422886666	13.925751346597778
4	25.41826096515335	27.534422337034187	31.613076838518197	15.434239859294275
5	22.99098164229966	30.569233813345058	31.08550071452127	15.354283829834012
6	21.189543805650217	39.8093789161262	28.56546114103551	10.435616137188083
7	84.11019017258437	3.8218489611959985	9.996227327690448	2.0717335385291853
8	83.77281301527977	3.4189205232494224	9.913756183357151	2.894510278113664
9	77.49054853248325	5.5570671649994505	12.888468725953611	4.063915576563702
10	37.642123777069365	26.637405738155433	20.861593932065517	14.858876552709685
11	31.384183796856107	25.87189622952622	26.994552050126412	15.749367923491262
12	27.4965373199956	23.40959876882489	30.618533582499722	18.475330328679785
13	22.667587116631857	27.450720017588214	32.309306364735626	17.572386501044303
14	20.18425414971969	29.835101681873144	32.44359239309663	17.53705177531054
15	19.662693195558976	24.037075959107398	39.028057601407056	17.27217324392657
16	21.845232494228867	22.70195449049137	36.7152775640321	18.737535451247663
17	21.7857315598549	22.902671210289107	31.407312300758495	23.904284929097503
18	23.669834011212487	23.487145212707485	33.336493349455864	19.506527426624164
19	26.74295262174343	25.477128723755083	28.61813784764208	19.161780806859404
20	28.190286907771796	24.977359569088712	27.35739694404749	19.47495657909201
21	25.20867978454436	26.6019830713422	29.387217764098057	18.802119380015387
22	24.11557216664835	23.95052434868638	29.22457953171375	22.70932395295152
23	22.28437506870397	27.188708365395186	28.4024535561174	22.124463009783447
24	21.539725184126635	25.353712212817413	33.617447510168184	19.489115092887765
25	21.589728481917113	26.019320655161042	31.661215785423767	20.729735077498077
26	20.329057931186107	28.462658019127186	30.047250742002856	21.16103330768385
27	20.92009233813345	27.248086182257886	30.2086050346268	21.623216444981864
28	20.079111795097283	26.99289875783225	32.78290425414972	20.145085192920742
29	21.21940859624052	25.220921182807523	32.286371331208095	21.27329888974387
30	22.18122018247774	26.740402330438606	31.592340331977574	19.48603715510608
31	25.040747499175552	25.89462020446301	29.0345036825327	20.030128613828737
32	25.42174343190063	26.382007255139055	28.448130152797624	19.74811916016269
33	24.32328899637243	25.93732439265692	28.881837968561065	20.857548642409583
34	22.451621413652852	26.96166208640211	29.8216642849291	20.76505221501594
35	21.498410464988456	26.58390238540178	31.127096845113773	20.790590304495986
36	25.228906232824006	27.30285588655601	28.14471584038694	19.323522040233044
37	22.246824227767394	28.03250302297461	29.705898647905904	20.014774101352096
38	22.523680334176102	28.681596130592503	28.359925250082448	20.43479828514895
39	21.973169176651645	27.909772452456856	28.428888644608115	21.68816972628339
40	23.666703308783116	26.061444432230406	29.501505990986036	20.770346268000438
41	20.491203693525335	25.90805760140706	29.616022864680662	23.98471584038694
42	22.806586786852808	26.81949653732	27.83889194239859	22.535024733428603
43	21.985533692426074	25.869345938221393	29.679445971199296	22.465674398153237
44	22.406050346268	27.6236297680554	28.653789161261955	21.316530724414644
45	21.02196328459932	28.963447290315486	27.778036715400685	22.236552709684513
46	22.688182917445314	27.969801033307682	27.963293393426405	21.3787226558206
47	22.09018357700341	27.26539298669891	28.86344069473453	21.780982741563154
48	22.256884687259536	26.473149389908762	29.836614268440144	21.433351654391558
49	22.38909530614488	25.464641090469385	29.940367154006815	22.20589644937892
50	21.435743651753324	27.68260305595251	29.454052984500382	21.427600307793778
51	21.027310102231507	28.182337034187093	28.26201165219303	22.52834121138837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4873.0
1	6307.5
2	7742.0
3	46990.0
4	86238.0
5	60453.0
6	34668.0
7	35595.5
8	36523.0
9	38455.0
10	40387.0
11	41542.0
12	42697.0
13	42438.0
14	42179.0
15	41311.5
16	40444.0
17	38769.0
18	37094.0
19	36608.0
20	36122.0
21	35069.0
22	34016.0
23	35098.0
24	36180.0
25	41757.5
26	49458.0
27	51581.0
28	58580.5
29	65580.0
30	77173.5
31	88767.0
32	105462.5
33	122158.0
34	135048.0
35	147938.0
36	165279.0
37	182620.0
38	205353.0
39	228086.0
40	263094.0
41	298102.0
42	333235.0
43	368368.0
44	413817.0
45	459266.0
46	466161.5
47	473057.0
48	452714.5
49	432372.0
50	408885.0
51	385398.0
52	359966.0
53	334534.0
54	310545.0
55	286556.0
56	270380.0
57	254204.0
58	233904.0
59	213604.0
60	198817.0
61	184030.0
62	169596.0
63	155162.0
64	142872.5
65	130583.0
66	112727.5
67	94872.0
68	81310.0
69	67748.0
70	57647.0
71	47546.0
72	41395.0
73	35244.0
74	28880.5
75	18059.0
76	13601.0
77	10792.5
78	7984.0
79	5934.0
80	3884.0
81	2963.5
82	2043.0
83	1556.5
84	1070.0
85	708.5
86	347.0
87	270.5
88	194.0
89	123.5
90	53.0
91	45.0
92	37.0
93	26.5
94	16.0
95	10.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5685625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.750215074902385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49282780415662	16.494932738484298
2	7.451418034434047	3.0923705365502574
3	2.6667321897710687	1.6600579945474525
4	1.3581988784753425	1.1273167537141826
5	0.9183622484356266	0.9528107085855095
6	0.6596795847339814	0.8213095958251444
7	0.5058189593202531	0.7347096536400987
8	0.41341834979549436	0.6862815739334188
9	0.3298351574532298	0.6159735410776929
>10	3.813653113639787	18.45137718184351
>50	1.3439961729141305	20.195933978581373
>100	1.0391536129104448	32.7179853354904
>500	0.004834125775055799	0.6841263118213198
>1k	0.0018991208362565586	0.7014654636869202
>5k	8.632367437529811E-5	0.1262641417106075
>10k+	8.632367437529811E-5	0.9370844905079346
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52315	0.9201275145652412	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7049	0.12397933384632295	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.2764647686050346E-5	0.0	0.0	0.04900076948444542	0.0
2	5.2764647686050346E-5	0.0	0.0	0.16944487193580302	0.0
3	7.035286358140047E-4	0.0	0.0	0.2989644937891613	0.0
4	7.035286358140047E-4	0.0	0.0	0.49983950752995493	0.0
5	7.035286358140047E-4	0.0	0.0	0.8541189403099924	0.0
6	7.035286358140047E-4	0.0	0.0	1.6613652852588765	0.0
7	7.035286358140047E-4	0.0	0.0	2.070607892711883	0.0
8	7.035286358140047E-4	0.0	0.0	2.98901176211938	0.0
9	7.035286358140047E-4	0.0	0.0	3.50819830713422	0.0
10	7.035286358140047E-4	0.0	0.0	4.225164339892272	0.0
11	7.035286358140047E-4	0.0	0.0	4.6616686819830715	0.0
12	7.035286358140047E-4	0.0	0.0	5.052830603495658	0.0
13	7.035286358140047E-4	0.0	0.0	5.272437067165	0.0
14	7.035286358140047E-4	0.0	0.0	5.38224029899967	0.0
15	7.035286358140047E-4	0.0	0.0	5.483108717159503	0.0
16	7.035286358140047E-4	0.0	0.0	5.64448059799934	0.0
17	7.035286358140047E-4	0.0	0.0	5.81174453116412	0.0
18	7.035286358140047E-4	0.0	0.0	6.066439485544685	0.0
19	7.035286358140047E-4	0.0	0.0	6.189381114653182	0.0
20	7.035286358140047E-4	0.0	0.0	6.324564142024843	0.0
21	7.035286358140047E-4	0.0	0.0	6.475471034406947	0.0
22	7.035286358140047E-4	0.0	0.0	6.638355501813785	0.0
23	7.035286358140047E-4	0.0	0.0	6.822293063647356	0.0
24	7.035286358140047E-4	0.0	0.0	6.973727602506321	0.0
25	7.035286358140047E-4	0.0	0.0	7.104196988018028	0.0
26	7.035286358140047E-4	0.0	0.0	7.234982961415851	0.0
27	7.035286358140047E-4	0.0	0.0	7.370412223810047	0.0
28	7.035286358140047E-4	0.0	0.0	7.514899417390349	0.0
29	7.035286358140047E-4	0.0	0.0	7.673070242937232	0.0
30	7.035286358140047E-4	0.0	0.0	7.906694514675167	0.0
31	7.035286358140047E-4	0.0	0.0	8.070950862921842	0.0
32	7.035286358140047E-4	0.0	0.0	8.245285258876553	0.0
33	7.035286358140047E-4	0.0	0.0	8.41372760250632	0.0
34	7.035286358140047E-4	0.0	0.0	8.576418599538309	0.0
35	7.035286358140047E-4	0.0	0.0	8.74980323183467	0.0
36	7.035286358140047E-4	0.0	0.0	8.911157524458613	0.0
37	7.035286358140047E-4	0.0	0.0	9.079423985929427	0.0
38	7.035286358140047E-4	0.0	0.0	9.262763548422557	0.0
39	7.211168517093547E-4	0.0	0.0	9.506993514345389	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35400	0.0	41.593224	1
CGTAAGG	765	0.0	37.647057	2
GGGCGAT	15790	0.0	37.376507	7
CACGACC	2385	0.0	37.35849	27
AGGGCGA	8125	0.0	36.996925	6
TGGGCGA	4620	0.0	36.915585	6
ACACGAC	2385	0.0	36.60377	26
AGGGTAC	2930	0.0	36.399315	6
TATAGCG	280	0.0	36.160713	1
CGGTCTA	405	0.0	36.11111	31
TACGGGA	1260	0.0	36.07143	4
CGTTCGG	1425	0.0	35.842106	45
GGCGATA	3250	0.0	35.79231	8
CGTTAGG	805	0.0	35.776398	2
ATAGGGC	3110	0.0	35.667202	4
GCGAGAC	2465	0.0	35.59838	21
CGAGACA	2465	0.0	35.59838	22
TAGCATA	3415	0.0	35.512444	30
GCGATAT	825	0.0	35.454548	9
GACACGA	2465	0.0	35.324547	25
>>END_MODULE